Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

NREL researchers use imaging technologies to solve puzzle of plant architecture

28.11.2012
Breakthrough could help optimize capture of sugars for biofuels

Scientists at the U.S. Department of Energy's National Renewable Energy Laboratory (NREL) and the BioEnergy Science Center (BESC) combined different microscopic imaging methods to gain a greater understanding of the relationships between biomass cell wall structure and enzyme digestibility, a breakthrough that could lead to optimizing sugar yields and lowering the costs of making biofuels.

A paper on the breakthrough, "How Does Plant Cell Wall Nanoscale Architecture Correlate with Enzymatic Digestibility?" appears in the current issue of Science Magazine.

Principal Investigator Dr. Shi-You Ding of NREL said the imaging technologies allowed the interdisciplinary team of scientists to view the plants' architecture at scales ranging from millimeter to nanometer, a range of 1 million to one.

That allowed them to learn not just the plant cell wall architecture, but also the localization of the enzymes responsible for deconstruction of the cell wall polymers and the effects of enzyme action on the cell wall.

They didn't have to resort to wet chemistry, which ascertains the molecular makeup of a substance at the cost of destroying the spatial relationships. "The typical way to understand the structure of biomass is to break down all the individual components so they can be analyzed," Ding, a biologist, said. "The problem with that method is that then you don't know where all the components came from. You lose the structural integrity."

That's a crucial loss, because an understanding of how enzymes digest plants requires an understanding of where everything is inside the cell walls.

"Our imaging techniques gave us a deeper understanding of the cell wall structure and the process of enzyme hydrolysis of cell-wall carbohydrate polymers to release simple sugars," Ding said. "That allows us to optimize the process and reduce costs."

Dr. Paul Gilna, the director of the BESC, in which the project was conducted, added: "This work greatly improves our ability to closely examine the mechanisms behind the scientific improvements we have developed, all of which are targeted at enabling the emergence of a sustainable cellulosic biofuels industry." BESC is a multi-institutional Bioenergy Research Center supported by the Office of Biological and Environmental Research in the Department of Energy Office of Science.

The correlative imaging in real time allowed the team to assess the impact of lignin removal on biomass hydrolysis and to see the nanometer-scale changes in cell wall structure. And, that allowed them to see how those changes affected the rate at which enzymes from two different organisms digested the plant cell walls.

The aim in the biofuel industry is to access the plants' polymeric carbohydrate structures without damaging the basic molecules of which the polymers are constructed. "It's more like dis-assembling a building with wrenches, hammers and crowbars to recover re-useable bricks, wiring, pipes and structural steel than it is like using a wrecking ball or explosives," Gilna said. Enzymes, unlike typical harsh chemical catalysts, excel at this relatively gentle disassembly.

The NREL team examined two enzyme systems – one from a fungus, the other from a bacterium – both holding promise as biocatalysts for producing sugar intermediates for the biofuels industry.

The particular bacterial enzymes studied are organized through a large scaffolding protein into a multi-enzyme complex from which they make a coordinated attack on the cell walls. The separate fungal enzymes act more individualistically, although the ultimate result is cooperative in that case, as well.

The NREL team found that the easier the access to the cell walls, the better and faster the enzymes will digest the material.

In biofuels production, enzymes are needed to greatly speed up the chemical reactions that break down the biomass during fermentation.

The NREL scientists found that the gummy, poly-aromatic non-sugar lignin in plants interferes with enzymes' ability to access the polysaccharides in the cell wall – the stuff that both the enzymes and the industry want.

So, they concluded, ideal pre-treatment should focus on getting rid of the lignin while leaving the structural polysaccharides within the cell walls intact, thus leaving a relatively loose, porous native-like structure that allows easy access by the enzymes and rapid digestion, as opposed to pretreatments that remove some of the spongier carbohydrate polymers and allow the remainder to collapse into tighter and less-accessible structures. To continue the building dis-assembly and salvage analogy, removal of the lignin is like unlocking all of the doors in the building so that the workers can get in to pull out re-useable materials, but without collapsing the overall structure so that access is blocked.

By understanding the changing structure of the plant material, scientists can learn more about how enzymes work.

"The enzyme has evolved to deal with the real structure, not the pretreated, artificially decomposed one," Ding said. "So to understand how the enzyme goes about its business, it is really important to know where cell wall components are located, as well as the various modes of enzyme action."

"Then we can optimize the whole process," Ding said. "By observing where cellulase enzymes are localized and the nanostructural changes in the plant cell wall architecture that their actions produce, we hope to suggest rational strategies for more cost effective pretreatments and better enzymes."

NREL is the U.S. Department of Energy's primary national laboratory for renewable energy and energy efficiency research and development. NREL is operated for DOE by the Alliance for Sustainable Energy, LLC.

Visit NREL online at www.nrel.gov

For further information contact NREL Public Relations at 303-275-4090.

David Glickson | EurekAlert!
Further information:
http://www.nrel.gov

More articles from Power and Electrical Engineering:

nachricht Scientists create biodegradable, paper-based biobatteries
08.08.2018 | Binghamton University

nachricht Ricocheting radio waves monitor the tiniest movements in a room
07.08.2018 | Duke University

All articles from Power and Electrical Engineering >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Unraveling the nature of 'whistlers' from space in the lab

A new study sheds light on how ultralow frequency radio waves and plasmas interact

Scientists at the University of California, Los Angeles present new research on a curious cosmic phenomenon known as "whistlers" -- very low frequency packets...

Im Focus: New interactive machine learning tool makes car designs more aerodynamic

Scientists develop first tool to use machine learning methods to compute flow around interactively designable 3D objects. Tool will be presented at this year’s prestigious SIGGRAPH conference.

When engineers or designers want to test the aerodynamic properties of the newly designed shape of a car, airplane, or other object, they would normally model...

Im Focus: Robots as 'pump attendants': TU Graz develops robot-controlled rapid charging system for e-vehicles

Researchers from TU Graz and their industry partners have unveiled a world first: the prototype of a robot-controlled, high-speed combined charging system (CCS) for electric vehicles that enables series charging of cars in various parking positions.

Global demand for electric vehicles is forecast to rise sharply: by 2025, the number of new vehicle registrations is expected to reach 25 million per year....

Im Focus: The “TRiC” to folding actin

Proteins must be folded correctly to fulfill their molecular functions in cells. Molecular assistants called chaperones help proteins exploit their inbuilt folding potential and reach the correct three-dimensional structure. Researchers at the Max Planck Institute of Biochemistry (MPIB) have demonstrated that actin, the most abundant protein in higher developed cells, does not have the inbuilt potential to fold and instead requires special assistance to fold into its active state. The chaperone TRiC uses a previously undescribed mechanism to perform actin folding. The study was recently published in the journal Cell.

Actin is the most abundant protein in highly developed cells and has diverse functions in processes like cell stabilization, cell division and muscle...

Im Focus: Lining up surprising behaviors of superconductor with one of the world's strongest magnets

Scientists have discovered that the electrical resistance of a copper-oxide compound depends on the magnetic field in a very unusual way -- a finding that could help direct the search for materials that can perfectly conduct electricity at room temperatur

What happens when really powerful magnets--capable of producing magnetic fields nearly two million times stronger than Earth's--are applied to materials that...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

Within reach of the Universe

08.08.2018 | Event News

A journey through the history of microscopy – new exhibition opens at the MDC

27.07.2018 | Event News

2018 Work Research Conference

25.07.2018 | Event News

 
Latest News

Staying in Shape

16.08.2018 | Life Sciences

Diving robots find Antarctic seas exhale surprising amounts of carbon dioxide in winter

16.08.2018 | Earth Sciences

Protein droplets keep neurons at the ready and immune system in balance

16.08.2018 | Life Sciences

VideoLinks
Science & Research
Overview of more VideoLinks >>>