Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

MTT’s barley gene transfer technique ready

03.12.2007
MTT Agrifood Research Finland has introduced an Agrobacterium-mediated gene transfer technique for barley. Genetically modified barley is at the same time the main opening in plant gene transfer research at MTT.

Development of gene transfer methods forms part of the Disease Resistant Barley project, which provides genomic tools for managing and monitoring barley net blotch. The project, which started in 2005 and runs until 2011, is implemented in cooperation between MTT, the University of Helsinki, and Boreal Plant Breeding Ltd.

In addition to gene transfer, our research focuses include gene mapping, isolation of genes on the basis of map location, new gene replication methods, and bioinformatics.

Net blotch is the number one disease afflicting barley in Finland. Although the area of the barley genome concerned with resistance to net blotch has been identified, there is no accurate information on the genes located there. Gene transfer enables us to identify and verify which genes leads to net blotch resistance in barley, and how these genes function.

– Once the gene is identified, we can use methods for plant breeding in which this characteristic can be identified from wild barley strains, for example, and new barley varieties produced through an intensified back cross programme, says Outi Manninen, the research scientist responsible for the Disease Resistant Barley project.

– Genomics makes use of gene transfer as a tool, and the production of a genetically modified (GM) variety of barley is not the actual objective. New information gleaned from the research can nevertheless be exploited in the development of GM varieties.

Marker genes transferred

The MTT plant genomics team employed Agrobacterium as the means of gene transfer. Agrobacterium is a general soil bacterium with an excellent natural capacity for gene transfer. The gene transferred to barley is a marker gene producing fluorescent protein, the colour helping to track gene function.

The project has utilized retrotransposon promoters areas isolated from the barley genome, which have been extensively researched by MTT research scientist Professor Alan Schulman. Promoters have a significant role in gene function, as they regulate at what intensity, what stage and in which parts of the plant the genes will function.

Promoter areas used in transfers are mostly derived from maize or the cauliflower mosaic virus. It is hoped the usual phenomenon of promoters turning off if many gene copies are transferred simultaneously will be avoided with the aid of new Finnish applications.

Sufficiently efficient

Outi Manninen says that all plants produced from embryo cell tissue in MTT research have proved to be genetically modified. – The method has now acquired an ample degree of efficiency and is very effective.

Juha Heikkilae | alfa
Further information:
http://www.mtt.fi/english/

More articles from Agricultural and Forestry Science:

nachricht Cereals use chemical defenses in a multifunctional manner against different herbivores
06.12.2018 | Max-Planck-Institut für chemische Ökologie

nachricht Can rice filter water from ag fields?
05.12.2018 | American Society of Agronomy

All articles from Agricultural and Forestry Science >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Data use draining your battery? Tiny device to speed up memory while also saving power

The more objects we make "smart," from watches to entire buildings, the greater the need for these devices to store and retrieve massive amounts of data quickly without consuming too much power.

Millions of new memory cells could be part of a computer chip and provide that speed and energy savings, thanks to the discovery of a previously unobserved...

Im Focus: An energy-efficient way to stay warm: Sew high-tech heating patches to your clothes

Personal patches could reduce energy waste in buildings, Rutgers-led study says

What if, instead of turning up the thermostat, you could warm up with high-tech, flexible patches sewn into your clothes - while significantly reducing your...

Im Focus: Lethal combination: Drug cocktail turns off the juice to cancer cells

A widely used diabetes medication combined with an antihypertensive drug specifically inhibits tumor growth – this was discovered by researchers from the University of Basel’s Biozentrum two years ago. In a follow-up study, recently published in “Cell Reports”, the scientists report that this drug cocktail induces cancer cell death by switching off their energy supply.

The widely used anti-diabetes drug metformin not only reduces blood sugar but also has an anti-cancer effect. However, the metformin dose commonly used in the...

Im Focus: New Foldable Drone Flies through Narrow Holes in Rescue Missions

A research team from the University of Zurich has developed a new drone that can retract its propeller arms in flight and make itself small to fit through narrow gaps and holes. This is particularly useful when searching for victims of natural disasters.

Inspecting a damaged building after an earthquake or during a fire is exactly the kind of job that human rescuers would like drones to do for them. A flying...

Im Focus: Topological material switched off and on for the first time

Key advance for future topological transistors

Over the last decade, there has been much excitement about the discovery, recognised by the Nobel Prize in Physics only two years ago, that there are two types...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

ICTM Conference 2019: Digitization emerges as an engineering trend for turbomachinery construction

12.12.2018 | Event News

New Plastics Economy Investor Forum - Meeting Point for Innovations

10.12.2018 | Event News

EGU 2019 meeting: Media registration now open

06.12.2018 | Event News

 
Latest News

Data use draining your battery? Tiny device to speed up memory while also saving power

14.12.2018 | Power and Electrical Engineering

Tangled magnetic fields power cosmic particle accelerators

14.12.2018 | Physics and Astronomy

In search of missing worlds, Hubble finds a fast evaporating exoplanet

14.12.2018 | Physics and Astronomy

VideoLinks
Science & Research
Overview of more VideoLinks >>>