Reseachers at The Ohio State University have demonstrated that Target Region Amplification Polymorphism, or TRAP, is an effective method for preserving the important genetic diversity of ornamental flower collections.
Pelargonium, commonly know as geranium, are some of the most popular flowers the world. So popular, in fact, that the Royal Horticultural Society listed more than 3,000 varieties of geranium in their 2004 distribution catalogue. Sold in hanging baskets, flats and decorative pots, geranium plants accounted for more than $206 million in wholesale revenue in the U.S. during 2004. Essential oils from some scented geraniums are finding new uses in perfumes and food flavorings.
There are over 280 documented species of Pelargonium. The interest in breeding has resulted in many novel and improved cultivars. According to Rose Palumbo, Department of Plant Pathology at The Ohio State University (OSU), ornamental plants like the geranium are often bred for their ornamental qualities rather than their ability to survive in diverse environments.
Palumbo and a team of researchers recently completed a study of Pelargonium grown at The Ornamental Plant Germplasm Center (OPGC) at Ohio State. OPGC collects heirloom cultivars, breeding lines and wild species.
Intending to find a way to preserve the genetic diversity of Pelagonium, the team tested a method known as Target Region Amplification Polymorphism, or TRAP. Palumbo explained, "The TRAP method uses molecuar markers targeted to a specific gene. Target sequences that have been generally successful in most plants tested the applicability of this method to OSU's geranium collection. Using TRAP allowed us to divide the population into groups of similiar species and groups known to share parents."
Palumbo continued, "TRAP has the advantage of producing a large number of markers through use of sequence information that is already available. Our first goal was to determine the feasibility of TRAP for the analysis of this large collection, so that in the future the most diverse genotypes may be retained. To achieve this goal, we first modified existing DNA extraction techniques to account for the high levels of phenolic compounds present in some Pelargonium species. Second, we evaluated the TRAP procedure using the DNA isolated from 46 accessions. Based on these results, the molecular analysis of the collection was completed, and the collection has decreased in size by approximately 25%. Continuing analyses should shrink the collection from approximately 800 plants to close to 200 plants by the end of this year."
Using the TRAP method will allow the OPGC to streamline their collection of geraniums into a much more manageable size. According to Palumbo, the smaller collection will be more efficient to maintain, while still providing the diverse genetic resources needed by breeders and researchers.
Michael W. Neff | EurekAlert!
Trees and climate change: Faster growth, lighter wood
14.08.2018 | Technische Universität München
Animals and fungi enhance the performance of forests
01.08.2018 | Deutsches Zentrum für integrative Biodiversitätsforschung (iDiv) Halle-Jena-Leipzig
There are currently great hopes for solid-state batteries. They contain no liquid parts that could leak or catch fire. For this reason, they do not require cooling and are considered to be much safer, more reliable, and longer lasting than traditional lithium-ion batteries. Jülich scientists have now introduced a new concept that allows currents up to ten times greater during charging and discharging than previously described in the literature. The improvement was achieved by a “clever” choice of materials with a focus on consistently good compatibility. All components were made from phosphate compounds, which are well matched both chemically and mechanically.
The low current is considered one of the biggest hurdles in the development of solid-state batteries. It is the reason why the batteries take a relatively long...
New design tool automatically creates nanostructure 3D-print templates for user-given colors
Scientists present work at prestigious SIGGRAPH conference
Most of the objects we see are colored by pigments, but using pigments has disadvantages: such colors can fade, industrial pigments are often toxic, and...
Scientists at the University of California, Los Angeles present new research on a curious cosmic phenomenon known as "whistlers" -- very low frequency packets...
Scientists develop first tool to use machine learning methods to compute flow around interactively designable 3D objects. Tool will be presented at this year’s prestigious SIGGRAPH conference.
When engineers or designers want to test the aerodynamic properties of the newly designed shape of a car, airplane, or other object, they would normally model...
Researchers from TU Graz and their industry partners have unveiled a world first: the prototype of a robot-controlled, high-speed combined charging system (CCS) for electric vehicles that enables series charging of cars in various parking positions.
Global demand for electric vehicles is forecast to rise sharply: by 2025, the number of new vehicle registrations is expected to reach 25 million per year....
17.08.2018 | Event News
08.08.2018 | Event News
27.07.2018 | Event News
21.08.2018 | Medical Engineering
21.08.2018 | Physics and Astronomy
21.08.2018 | Power and Electrical Engineering