After the mare died at the Cornell Hospital for Animals, veterinarian Sylvia Bedford-Guaus and her laboratory team " graduate student Lori McPartlin and technician Stephanie Twomey " made use of special tools and their expertise from years of research to scrape the wall of each follicle in both of the ovaries obtained from the mare, Rebaqua.
"The process is time-sensitive and very intricate," said Bedford-Guaus, explaining that the eggs (oocytes) can only be seen microscopically and must be collected from the ovary within a few hours.
"There were other approaches, such as shipping the entire ovary, but none that could offer the success rates we had with this procedure," she said, adding that when equine ovaries have been shipped before, few pregnancies have resulted in no live births because the oocytes do not stay viable within the ovaries.
Bedford-Guaus searched for oocytes under a dissecting microscope and washed them in a special medium. The process took more than three hours. "Racing against the clock," said Bedford-Guaus, the team packaged nine oocytes for overnight shipment to veterinarian Katrin Hinrichs Equine Embryo Laboratory at Texas A&M University, where the researchers had the expertise needed to fertilize the eggs.
In Texas, the oocytes were incubated in a medium that stimulated five of the oocytes to mature; they were then fertilized with frozen-thawed sperm from the third-ranked barrel-racing stallion in the nation, Frenchmans Guy. This procedure, performed by veterinarian Young-Ho Choi, used a micromanipulation technique termed intracytoplasmic sperm injection. The fertilized eggs were cultured for seven days. Two developed into embryos, which were then sent to veterinarian David Hartman at the Hartman Equine Reproduction Center in Whitesboro, Texas, who transferred them to a surrogate mare that was at the right stage of the cycle to carry a pregnancy. The mare was then purchased by Rebaqua's owner, Kristin Contro of Binghamton, who had the pregnant mare shipped to New York.
"Because there are so many horses in the world, prior to Rebaqua's never felt it was necessary to breed a horse," said Contro, who began riding when she was 10 and has owned more than 10 horses. "Rebaqua was as close to perfect as you could get. She was bred really well, so her genes were superb. Her confirmation was perfect. And, she was a champion barrel racer. I am hopeful that her foal will also have her heart. That's not something you can breed for. They either have it or they don't."
(Text by Stephanie Specchio of Cornell's College of Veterinary Medicine.)
Joe Schwartz | Newswise Science News
New insight into why Pierce's disease is so deadly to grapevines
11.06.2018 | University of California - Davis
Where are Europe’s last primary forests?
29.05.2018 | Humboldt-Universität zu Berlin
In a recent publication in the renowned journal Optica, scientists of Leibniz-Institute of Photonic Technology (Leibniz IPHT) in Jena showed that they can accurately control the optical properties of liquid-core fiber lasers and therefore their spectral band width by temperature and pressure tuning.
Already last year, the researchers provided experimental proof of a new dynamic of hybrid solitons– temporally and spectrally stationary light waves resulting...
Scientists from the University of Freiburg and the University of Basel identified a master regulator for bone regeneration. Prasad Shastri, Professor of...
Moving into its fourth decade, AchemAsia is setting out for new horizons: The International Expo and Innovation Forum for Sustainable Chemical Production will take place from 21-23 May 2019 in Shanghai, China. With an updated event profile, the eleventh edition focusses on topics that are especially relevant for the Chinese process industry, putting a strong emphasis on sustainability and innovation.
Founded in 1989 as a spin-off of ACHEMA to cater to the needs of China’s then developing industry, AchemAsia has since grown into a platform where the latest...
The BMBF-funded OWICELLS project was successfully completed with a final presentation at the BMW plant in Munich. The presentation demonstrated a Li-Fi communication with a mobile robot, while the robot carried out usual production processes (welding, moving and testing parts) in a 5x5m² production cell. The robust, optical wireless transmission is based on spatial diversity; in other words, data is sent and received simultaneously by several LEDs and several photodiodes. The system can transmit data at more than 100 Mbit/s and five milliseconds latency.
Modern production technologies in the automobile industry must become more flexible in order to fulfil individual customer requirements.
An international team of scientists has discovered a new way to transfer image information through multimodal fibers with almost no distortion - even if the fiber is bent. The results of the study, to which scientist from the Leibniz-Institute of Photonic Technology Jena (Leibniz IPHT) contributed, were published on 6thJune in the highly-cited journal Physical Review Letters.
Endoscopes allow doctors to see into a patient’s body like through a keyhole. Typically, the images are transmitted via a bundle of several hundreds of optical...
13.06.2018 | Event News
08.06.2018 | Event News
05.06.2018 | Event News
22.06.2018 | Materials Sciences
22.06.2018 | Earth Sciences
22.06.2018 | Life Sciences