Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

New wrapping material enables high quality bioimaging

28.08.2017

A nanosheet made of organic polymers has been developed to prevent the drying and deforming of biological samples, thus enabling high-quality imaging under microscopes.

Be it cosmology or biology, the advancement of science largely relies on the advancement of measuring instruments and methodology. In the past couple of decades, scientists' passion to see the invisible has vastly improved microscopes and other equipment resulting in high-resolution images, three-dimensional images, and longer recording times of biological samples. However, current setups do not prevent them from drying and deforming during observations, resulting in blurred images.


This is the CYTOP® nanosheet developed in the study.

Credit: Zhang H. et al., Advanced Materials, Aug. 11, 2017

Scientists from Hokkaido University and Tokai University in Japan have developed a nanosheet made of a fluorine-containing polymer known as CYTOP® and investigated its effectiveness in producing clear images when wrapped around biological samples. CYTOP® is a commercially available chemical agent developed by Asahi Glass Co., Ltd.

The researchers successfully produced a nanosheet of CYTOP® by spreading its solution on a layer of poly-vinyl-alcohol (PVA). The nano-thin layer of CYTOP® was easily detached from the PVA layer by soaking them in water. The thickness of the nanosheet could be adjusted by changing the concentration of its solution. The nanosheet retained CYTOP®'s high water repellent properties which should help retain a sample's water content when used as wrapper.

The team then tested the nanosheet's wrapping properties by using alginate hydrogel to simulate biological samples such as tissue. They found that the gel retained 60% of its water content after 24 hours when wrapped whereas the unwrapped gel became totally dehydrated after about 10 hours.

Experimenting with various thicknesses, they found that a 133-nm-thick sheet provides sufficient water retention and surface adhesion which is necessary for fixing samples to the cover slip.

They next applied an actual biological sample: 1-mm thick brain slices from mice. Without a CYTOP® wrap, evaporation of the embedded water caused local, non-uniform sample shrinkage, leading to blurred images. By wrapping the brain slices in a CYTOP® nanosheet, however, images with a high spatial resolution could be obtained from scanning a large area (more than 750 μm x 750 μm) over a long time (about 2 hours).

Similar results were obtained even when the sample was embedded in agarose gel -- a common technique used for mounting biological tissues that could disturb the clarity of taransparentized samples -- without disturbing the clarity.

"The wrapping technique is still at an early stage, but it establishes and verifies the superiority of nanosheet wrapping for tissue imaging," say the researchers of the paper published in Advanced Materials.

Media Contact

Naoki Namba
81-117-062-185

 @hokkaido_uni

https://www.global.hokudai.ac.jp/ 

Naoki Namba | EurekAlert!

More articles from Materials Sciences:

nachricht Epoxy compound gets a graphene bump
14.11.2018 | Rice University

nachricht Automated adhesive film placement and stringer integration for aircraft manufacture
15.11.2018 | Fraunhofer IFAM

All articles from Materials Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: UNH scientists help provide first-ever views of elusive energy explosion

Researchers at the University of New Hampshire have captured a difficult-to-view singular event involving "magnetic reconnection"--the process by which sparse particles and energy around Earth collide producing a quick but mighty explosion--in the Earth's magnetotail, the magnetic environment that trails behind the planet.

Magnetic reconnection has remained a bit of a mystery to scientists. They know it exists and have documented the effects that the energy explosions can...

Im Focus: A Chip with Blood Vessels

Biochips have been developed at TU Wien (Vienna), on which tissue can be produced and examined. This allows supplying the tissue with different substances in a very controlled way.

Cultivating human cells in the Petri dish is not a big challenge today. Producing artificial tissue, however, permeated by fine blood vessels, is a much more...

Im Focus: A Leap Into Quantum Technology

Faster and secure data communication: This is the goal of a new joint project involving physicists from the University of Würzburg. The German Federal Ministry of Education and Research funds the project with 14.8 million euro.

In our digital world data security and secure communication are becoming more and more important. Quantum communication is a promising approach to achieve...

Im Focus: Research icebreaker Polarstern begins the Antarctic season

What does it look like below the ice shelf of the calved massive iceberg A68?

On Saturday, 10 November 2018, the research icebreaker Polarstern will leave its homeport of Bremerhaven, bound for Cape Town, South Africa.

Im Focus: Penn engineers develop ultrathin, ultralight 'nanocardboard'

When choosing materials to make something, trade-offs need to be made between a host of properties, such as thickness, stiffness and weight. Depending on the application in question, finding just the right balance is the difference between success and failure

Now, a team of Penn Engineers has demonstrated a new material they call "nanocardboard," an ultrathin equivalent of corrugated paper cardboard. A square...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

“3rd Conference on Laser Polishing – LaP 2018” Attracts International Experts and Users

09.11.2018 | Event News

On the brain’s ability to find the right direction

06.11.2018 | Event News

European Space Talks: Weltraumschrott – eine Gefahr für die Gesellschaft?

23.10.2018 | Event News

 
Latest News

Purdue cancer identity technology makes it easier to find a tumor's 'address'

16.11.2018 | Health and Medicine

Good preparation is half the digestion

16.11.2018 | Life Sciences

Microscope measures muscle weakness

16.11.2018 | Life Sciences

VideoLinks
Science & Research
Overview of more VideoLinks >>>