Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Lab studies show two proteins prevented progressive nerve cell loss in Parkinson’s disease

09.09.2004


In recent years, scientists have made important strides in developing drugs that help patients manage the symptoms of Parkinson’s Disease – a chronic, progressive movement disorder affecting as many as one million Americans. But despite their effectiveness, the drugs don’t stop Parkinson’s disease from progressing, causing patients’ symptoms to eventually grow worse in spite of medication.



Now, researchers at Cedars-Sinai Medical Center have found that two specific proteins – "Sonic Hedgehog" and "Gli-1" – delivered via a genetically engineered virus into the brains of laboratory rats, prevented the progressive degeneration of nerve cells in the brain that cause Parkinson’s disease. The study, published in the September issue of the journal, Molecular Therapy, may lead to a new way to treat patients with advanced Parkinson’s Disease.

"Our results establish, for the first time, that viral transfer of Sonic hedgehog and Gli-1 - two proteins that are involved in early brain development, but are no longer present in the adult brain – may provide a new strategy to prevent progressive degeneration of the nerve cells in the brain that cause Parkinson’s disease," said Pedro Lowenstein, M.D., Ph.D., Director of the Gene Therapeutics Research Institute at Cedars-Sinai Medical Center, and a Professor of Medicine and Pharmacology at UCLA.


Parkinson’s disease occurs when the nerve cells in the part of the brain known as the "substantia nigra" that produce a chemical called dopamine begin to malfunction and progressively die. Dopamine acts as a chemical messenger to send signals to a part of the brain called the basal ganglia that controls movement and coordination. But as more of these cells die, less and less dopamine is produced, causing Parkinson’s disease.

To treat the symptoms of Parkinson’s disease, doctors rely on various types of drugs that work by helping to replenish dopamine. A drug called L-DOPA (levodopa), for example, is absorbed in the brain and changed into dopamine. Other drugs, work differently by either prolonging the effect of levodopa-formed dopamine or by actually mimicking dopamine. But regardless of how they work, patients often have to take more than one drug to either enhance the drug’s effects or to reduce the side effects that many of the drugs cause. As more cells die during the progression of the disease, these drugs lose their effectiveness.

"Because these drugs don’t stop the disease from progressing, we used a genetically engineered viral vector to deliver very specific molecules to see whether they would prevent the nerve cells in the brain from dying in adult rats with Parkinson’s," commented Maria Castro, Ph.D., Co-Director of the Gene Therapeutic Research Institute at Cedars-Sinai and a senior author of the study. "Ultimately, because we found that two of these molecules prevented cell death, we aim to translate this type of gene therapy into a clinical trial to determine whether it will stop the progression of the disease. Importantly, Sonic Hedgehog and Gli1, display novel mechanisms of action compared to other drugs utilized so far."

Genetically engineered viruses are used to transport genes and/or proteins into cells and have been used in gene therapy research for the last ten years. Just like a viral infection, they work by tricking cells into accepting them as part of their own genetic coding. The trick has been to alter the virus so that it delivers the desired genetic material, while removing any characteristics that make the virus dangerous.

To make viruses safe and effective, scientists remove the genetic coding that causes infection and engineer them so that they stop reproducing. The adenoviral vector used in this study, for example, has been genetically manipulated to selectively express proteins that can protect the neurons that are damaged during the course of Parkinson’s disease.

"Currently, we are constructing other versions of this virus that could eventually be used in clinical trials in humans that are safe even when the body mounts an immune response, and that could be switched ’on’ and ’off’ at will," said Dr. Castro. "In other words, these new viral vectors would effectively resist any attack by the immune system and have built-in switches that allow the doctor to regulate the delivery of the neuroprotective proteins as needed."

In the laboratory study, the investigators used an adenoviral vector to test the effectiveness of three proteins: Sonic Hedgehog (Shh), a signaling molecule that plays a key role in the development of the brain but is no longer present in the adult brain; Gli-1, a protein that turns genes on and off within the same signaling pathway; and Nurr1, a protein receptor that is needed to produce substantia nigra neurons in the brain.

To determine whether any of the three proteins would prevent nerve cells in the brain from dying, the investigators injected Shh, Gli-1 and Nurr1 into the brains of laboratory rats with Parkinson’s disease. The investigators found that nerve cells in the rats treated with Shh and Gli1, were protected, while no effect was seen in those treated with Nurr1.

"Given that these proteins are only present in the developing brain, our study demonstrates that we were able to use a genetically engineered virus to deliver them into the adult brains of laboratory rats with Parkinson’s disease and that they significantly protected nerve cells from dying," said Dr. Lowenstein.

Kelli Hanley | EurekAlert!
Further information:
http://www.cshs.org

More articles from Studies and Analyses:

nachricht Study relating to materials testing Detecting damages in non-magnetic steel through magnetism
23.07.2018 | Technische Universität Kaiserslautern

nachricht Innovative genetic tests for children with developmental disorders and epilepsy
11.07.2018 | Christian-Albrechts-Universität zu Kiel

All articles from Studies and Analyses >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Color effects from transparent 3D-printed nanostructures

New design tool automatically creates nanostructure 3D-print templates for user-given colors
Scientists present work at prestigious SIGGRAPH conference

Most of the objects we see are colored by pigments, but using pigments has disadvantages: such colors can fade, industrial pigments are often toxic, and...

Im Focus: Unraveling the nature of 'whistlers' from space in the lab

A new study sheds light on how ultralow frequency radio waves and plasmas interact

Scientists at the University of California, Los Angeles present new research on a curious cosmic phenomenon known as "whistlers" -- very low frequency packets...

Im Focus: New interactive machine learning tool makes car designs more aerodynamic

Scientists develop first tool to use machine learning methods to compute flow around interactively designable 3D objects. Tool will be presented at this year’s prestigious SIGGRAPH conference.

When engineers or designers want to test the aerodynamic properties of the newly designed shape of a car, airplane, or other object, they would normally model...

Im Focus: Robots as 'pump attendants': TU Graz develops robot-controlled rapid charging system for e-vehicles

Researchers from TU Graz and their industry partners have unveiled a world first: the prototype of a robot-controlled, high-speed combined charging system (CCS) for electric vehicles that enables series charging of cars in various parking positions.

Global demand for electric vehicles is forecast to rise sharply: by 2025, the number of new vehicle registrations is expected to reach 25 million per year....

Im Focus: The “TRiC” to folding actin

Proteins must be folded correctly to fulfill their molecular functions in cells. Molecular assistants called chaperones help proteins exploit their inbuilt folding potential and reach the correct three-dimensional structure. Researchers at the Max Planck Institute of Biochemistry (MPIB) have demonstrated that actin, the most abundant protein in higher developed cells, does not have the inbuilt potential to fold and instead requires special assistance to fold into its active state. The chaperone TRiC uses a previously undescribed mechanism to perform actin folding. The study was recently published in the journal Cell.

Actin is the most abundant protein in highly developed cells and has diverse functions in processes like cell stabilization, cell division and muscle...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

LaserForum 2018 deals with 3D production of components

17.08.2018 | Event News

Within reach of the Universe

08.08.2018 | Event News

A journey through the history of microscopy – new exhibition opens at the MDC

27.07.2018 | Event News

 
Latest News

Smallest transistor worldwide switches current with a single atom in solid electrolyte

17.08.2018 | Physics and Astronomy

Robots as Tools and Partners in Rehabilitation

17.08.2018 | Information Technology

Climate Impact Research in Hannover: Small Plants against Large Waves

17.08.2018 | Life Sciences

VideoLinks
Science & Research
Overview of more VideoLinks >>>