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RBL-C5aR cell line

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23.07.2008

Scientists at the University of Göttingen developed a transfectant RBL-C5aR cell line which is able to express more than 1 million C5a receptors on its cell surface, showing a specific high Ca2+ signal. The generated RBL-cells express C5aR in a number of „flavours“ (FLAG-tagged, N-glycosilation deficient, phosphorylation-deficient, co-transfected with other GPCR). C5aR(CD88) binds to C5a, an important chemotactic protein, element of the biochemical cascade of the complement system. This cell line would make the development of a standardized assay easy, fast, reproducible and sensitive.

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