Well-established methods to obtain biocatalysts for re-use are e.g. the immobilization via adsorption onto suitable support materials, encapsulation in aqueous gels or cross-linked enzyme aggregates. All these recycling methods suffer from leaching of the enzyme and often from a substantial loss in activity. Laborious modifications of the enzyme are partly essential or highly-priced apparatus for efficient separation needs to be implemented. Another method is the usage of a liquid-liquid two-phase system, consisting of an aqueous medium and a water-immiscible organic solvent. But mass transfer limitations between the two immiscible phases are hardly narrowing the economically possible field of applications.
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