This procedure facilitates one of the most frequent routine tasks in molecular biology: the subcloning of nucleic acid fragments from one vector into another. As the only precondition for this, a source vector with two sequence sections (3) is required, both of which carry a recognition site for an outside cutter restriction enzyme and bordering nucleotides which form a specific base overlap with the outside cutter restriction enzyme during cleavage. The invention makes use of the fact that outside cutter restriction enzymes recognise a recognition site of 4-8 base pairs, while the cleavage site is located within a defined distance from the recognition site.
firstname.lastname@example.org | TechnologieAllianz e.V.
New Lithium Salts of Pentafluorophenylamide Anions as Electrolytes in Lithium Ionic Batteries
18.04.2017 | TechnologieAllianz e.V.
Gratings on glass surfaces
28.03.2017 | TechnologieAllianz e.V.
16.08.2017 | Event News
04.08.2017 | Event News
26.07.2017 | Event News
18.08.2017 | Life Sciences
18.08.2017 | Physics and Astronomy
18.08.2017 | Materials Sciences