New bioreactor for cultivation and pressure stimulation of cells and cell-matrix-composites
The invention concerns new bioreactor systems for cultivation and pressure stimulation of cell-matrix-composites.
A special aspect of the invention is that the device is composed in a way that generally available tissue culture plates (with up to 96 cavities) can be used as part of the new bioreactors. Through connection with a new device for pressure stimulation of the cells or composites a bioreactor system develops. This bioreactor system already has a lot of advantages compared to known systems because of the use of existing culture plates. However, it is also useful due to the fact that the stimulation of the cells or composites can take place with defined or undefined pressure and that durability and frequency of the pressure stimulation are freely programmable. The pressure stimulation can occur variably for every cavity. The change of medium and samples are carried out in a closed system and an easy cleaning is also possible. The invented bioreactor system also shows heating and/or cooling elements and means of pumping as well as sensors and control units for pH-value, temperature and pressure and runs either in the perfusion mode (continuous) or in the batch mode. In order to create pressure stimulations we use the generation of compressed air in one experiment and in an other, alternative one we use electromagnetic induction.
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