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Protein P4 (SLC10A4) as a marker protein for cholinergic neurons of the CNS

06.07.2007
The protein SLC10A4, also known as P4, occurs in numerous cholinergic neurons. Up to now, however, a method that allows for the detection of SLC10A in cholinergic neurons does not exist.<p>

The invention at hand discloses a procedure for the detection of cholinergic neurons in the CNS by means of the detection of SLC10A4. For this purpose, oligopeptides of the C terminus of SLC10A4 are coupled to KLH, and polyclonal antibodies against these antigens are subsequently formed. According to the present invention, polyclonal antibodies against SLC10A4 thus obtained are used for the detection of SLC10A4 in cholinergic neurons, for example by means of the sandwich method. The visualization of SLC10A4 can be carried out, for instance, via immunofluorescence or peroxidase staining.

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