Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Protein for the chemoenzymatic production of L-<i>threo</i>-hydroxyaspartate

26.06.2007

The invention at hand provides a novel protein for the chemoenzymatic and enantiomerically pure production of L-&lt;i&gt;threo&lt;/i&gt;-hydroxyaspartate from L-aspartate.&lt;p&gt; The naturally occurring protein AsnO (asparagine oxygenase) is part of the CDA biosynthesis gene cluster in Streptomyces coelicor. AsnO is an Fe&lt;sup&gt;2+&lt;/sup&gt; and &lt;font face="symbol"&gt;a&lt;/font&gt;-ketoglutarate-dependent hydroxylase. This hydroxylase acts as a building block for non-ribosomally produced CDA (?Calcium-dependent antibiotic?) in vivo. During the catalytic cycle, this class of enzymes couples the oxidative decomposition of &lt;font face="symbol"&gt;a&lt;/font&gt;-ketoglutarate to succinate and CO&lt;sub&gt;2&lt;/sub&gt; with the hydroxylation of the substrate (L-asparagine).&lt;p&gt; In the wild type of AsnO, the side chain of the amino acid residue Asp-241 thereby binds to the NH&lt;sub&gt;2&lt;/sub&gt; group of the carboxamide group of L-Asn. Surprisingly, it was found that directed mutagenesis of Asp-241 to Asn-241 (D241N) results in a binding site for the carboxyl group of an aspartate side chain. By means of this directed mutagenesis, the substrate specificity changes from asparagine to aspartate, and the mutated protein converts aspartate chemoenzymatically to L-threo-hydroxyaspartate in the presence of Fe2+ and a-ketoglutarate.&lt;p&gt; Thereby, AsnO D241N represents the mutant of the wild type AsnO according to the present invention.

Further information: PDF

TransMIT Gesellschaft für Technologietransfer mbH
Phone: +49 (0)641/943 64-12

Contact
Dr. Peter Stumpf

As Germany's association of technology- and patenttransfer agencies TechnologieAllianz e.V. is offering businesses access to the entire range of innovative research results of almost all German universities and numerous non-university research institutions. More than 2000 technology offers of 14 branches are beeing made accessable to businesses in order to assure your advance on the market. At www.technologieallianz.de a free, fast and non-bureaucratic access to all further offers of the German research landscape is offered to our members aiming to sucessfully transfer technologies.

info@technologieallianz.de | TechnologieAllianz e.V.
Further information:
http://www.technologieallianz.de/angebote.php?sort=sag&id=01045&lang=en
http://www.technologieallianz.de/

More articles from Technology Offerings:

nachricht New Lithium Salts of Pentafluorophenylamide Anions as Electrolytes in Lithium Ionic Batteries
18.04.2017 | TechnologieAllianz e.V.

nachricht Gratings on glass surfaces
28.03.2017 | TechnologieAllianz e.V.

All articles from Technology Offerings >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Explanation for puzzling quantum oscillations has been found

So-called quantum many-body scars allow quantum systems to stay out of equilibrium much longer, explaining experiment | Study published in Nature Physics

Recently, researchers from Harvard and MIT succeeded in trapping a record 53 atoms and individually controlling their quantum state, realizing what is called a...

Im Focus: Dozens of binaries from Milky Way's globular clusters could be detectable by LISA

Next-generation gravitational wave detector in space will complement LIGO on Earth

The historic first detection of gravitational waves from colliding black holes far outside our galaxy opened a new window to understanding the universe. A...

Im Focus: Entangled atoms shine in unison

A team led by Austrian experimental physicist Rainer Blatt has succeeded in characterizing the quantum entanglement of two spatially separated atoms by observing their light emission. This fundamental demonstration could lead to the development of highly sensitive optical gradiometers for the precise measurement of the gravitational field or the earth's magnetic field.

The age of quantum technology has long been heralded. Decades of research into the quantum world have led to the development of methods that make it possible...

Im Focus: Computer-Designed Customized Regenerative Heart Valves

Cardiovascular tissue engineering aims to treat heart disease with prostheses that grow and regenerate. Now, researchers from the University of Zurich, the Technical University Eindhoven and the Charité Berlin have successfully implanted regenerative heart valves, designed with the aid of computer simulations, into sheep for the first time.

Producing living tissue or organs based on human cells is one of the main research fields in regenerative medicine. Tissue engineering, which involves growing...

Im Focus: Light-induced superconductivity under high pressure

A team of scientists of the Max Planck Institute for the Structure and Dynamics of Matter (MPSD) at the Center for Free-Electron Laser Science in Hamburg investigated optically-induced superconductivity in the alkali-doped fulleride K3C60under high external pressures. This study allowed, on one hand, to uniquely assess the nature of the transient state as a superconducting phase. In addition, it unveiled the possibility to induce superconductivity in K3C60 at temperatures far above the -170 degrees Celsius hypothesized previously, and rather all the way to room temperature. The paper by Cantaluppi et al has been published in Nature Physics.

Unlike ordinary metals, superconductors have the unique capability of transporting electrical currents without any loss. Nowadays, their technological...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

Save the date: Forum European Neuroscience – 07-11 July 2018 in Berlin, Germany

02.05.2018 | Event News

Invitation to the upcoming "Current Topics in Bioinformatics: Big Data in Genomics and Medicine"

13.04.2018 | Event News

Unique scope of UV LED technologies and applications presented in Berlin: ICULTA-2018

12.04.2018 | Event News

 
Latest News

Supersonic waves may help electronics beat the heat

18.05.2018 | Power and Electrical Engineering

Keeping a Close Eye on Ice Loss

18.05.2018 | Information Technology

CrowdWater: An App for Flood Research

18.05.2018 | Information Technology

VideoLinks
Science & Research
Overview of more VideoLinks >>>