The present invention relates to a detection of pathogenic fungi using a GFP-labeled, catalytically inactive chitosanase, which retained the affinity and specificity towards chitosan. This new method provides a rapid, reliable and robust system.
Commercial Opportunities: Fungal infections are a widespread ailment throughout flora and fauna. In humans, in particular in immunosuppressed patients, the increased incidence of severe mycoses is a challenge.So far, many attempts were run to produce antisera or antibodies against chitosan but have been of limited success due to the lack of antigenicity connected to chitosan. Additionally, antibody production is accompanied by high costs. Consequently, there is an unmet medical need for a quick, easy and cheap in situ detection of chitosan. The mutein described in the invention is a high-affinity binder of chitosan that could be used for analytical or diagnostic purposes. Both phytopathological and medical applications are conceivable.
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