Methods for producing antibody libraries, selecting and humanizing antibodies

New method for producing a highly diverse antibody library, where pro-teins/antibodies can be selected for specific applications. A large and, compared from cell to cell, highly uniform number of antibodies is covalently attached to the surface of a eukaryotic cell. Using this technology, specific antibodies can be selected together with the presenting cell out of a high number of cells in a comparatively easy way. The technology can also be used humanizing a hybridoma cell line by enabling the homologous recombination of the constant do-mains of human immunoglobulins with the respective gene.

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