Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Method for isolation & accumulation of small RNAs for clinical marker development

23.02.2012
A large part of gene regulation in eukaryotes takes place at the level of RNA-molecules. A group of endogenous regulatory RNA-molecules, the so-called microRNAs (miRNAs) appear to be involved in the regulation of about 30% of all human genes. These “small RNA” molecules (sRNA) also play a role in the development of diseases such as cancer, diabetes or neurological disorders. Disease-associated changes of miRNA expression patterns provide new prognostic and diagnostic opportunities and are qualified for the development of respective clinical markers.

To detect miRNA-derived biomarkers a whole-RNA purification from the sample has to be performed. For an identification of yet unknown miRNAs it is required to clone and sequence the purified sRNAs. In most cases miRNAs which are present in a sample at low concentrations are further depleted during the purification steps or even get lost, which renders in the results of the analysis non-representative and increases the risk to overlook potential biomarkers. The present invention is based on the finding, that the plant-viral protein ’Helper component proteinase’ (HC-Pro) can be used for a binding of sRNAs including miRNAs. Thus, HC-Pro can be used in order to enrich and to isolate sRNAs from samples. In addition a cupin protein was identified which clearly improves the binding of HC-Pro for sRNAs.

Further Information: PDF

IMG Innovations-Management GmbH
Phone: +49 (0)631/31668-50

Contact
Dr. Klaus Kobek

info@technologieallianz.de | TechnologieAllianz e.V.
Further information:
http://www.technologieallianz.de/angebote.php?sort=sag&id=2122&lang=en
http://www.technologieallianz.de

More articles from Technology Offerings:

nachricht Intelligent Method and System for the Analysis of Optical Retardation
21.07.2015 | TechnologieAllianz e.V.

nachricht Vibration tests for objects
21.07.2015 | TechnologieAllianz e.V.

All articles from Technology Offerings >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Superfast fluorescence sets new speed record

Plasmonic device has speed and efficiency to serve optical computers

Researchers have developed an ultrafast light-emitting device that can flip on and off 90 billion times a second and could form the basis of optical computing.

Im Focus: Unlocking the rice immune system

Joint BioEnergy Institute study identifies bacterial protein that is key to protecting rice against bacterial blight

A bacterial signal that when recognized by rice plants enables the plants to resist a devastating blight disease has been identified by a multi-national team...

Im Focus: Smarter window materials can control light and energy

Researchers in the Cockrell School of Engineering at The University of Texas at Austin are one step closer to delivering smart windows with a new level of energy efficiency, engineering materials that allow windows to reveal light without transferring heat and, conversely, to block light while allowing heat transmission, as described in two new research papers.

By allowing indoor occupants to more precisely control the energy and sunlight passing through a window, the new materials could significantly reduce costs for...

Im Focus: Simulations lead to design of near-frictionless material

Argonne scientists used Mira to identify and improve a new mechanism for eliminating friction, which fed into the development of a hybrid material that exhibited superlubricity at the macroscale for the first time. Argonne Leadership Computing Facility (ALCF) researchers helped enable the groundbreaking simulations by overcoming a performance bottleneck that doubled the speed of the team's code.

While reviewing the simulation results of a promising new lubricant material, Argonne researcher Sanket Deshmukh stumbled upon a phenomenon that had never been...

Im Focus: NASA satellite camera provides 'EPIC' view of Earth

A NASA camera on the Deep Space Climate Observatory (DSCOVR) satellite has returned its first view of the entire sunlit side of Earth from one million miles away.

The color images of Earth from NASA's Earth Polychromatic Imaging Camera (EPIC) are generated by combining three separate images to create a...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

3rd Euro Bio-inspired - International Conference and Exhibition on Bio-inspired Materials

23.07.2015 | Event News

Clash of Realities – International Conference on the Art, Technology and Theory of Digital Games

10.07.2015 | Event News

World Conference on Regenerative Medicine in Leipzig: Last chance to submit abstracts until 2 July

25.06.2015 | Event News

 
Latest News

Young Scientist Discovers Magnetic Material Unnecessary to Create Spin Current

27.07.2015 | Materials Sciences

Superfast fluorescence sets new speed record

27.07.2015 | Information Technology

Ultra-Thin Hollow Nanocages Could Reduce Platinum Use in Fuel Cell Electrodes

27.07.2015 | Power and Electrical Engineering

VideoLinks
B2B-VideoLinks
More VideoLinks >>>