Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Inexpensive Enhancement of High-Resolution 3-D Fluorescence Microscopy: FAM Fluorescence Activation Microscopy

09.07.2013
Optical setups using two opposing micro-objectives have been propagated to enhance the axial resolution in 3-dimensional fluorescence microscopy using various illumination schemes. However, the coherent superposition of two counter propagating beams creates imaging artifacts, i.e. more or less pronounced side-maxima, which can only be effectively removed by arithmetic op-erations if their intensity is less than 50% of the main maximum.

Fluorescence Activation Microscopy (FAM) is a new alternative 3D fluorescence image technique besides the existing confocal or 2-photon excita-tion microscopy. In FAM, photo activatable dyes are used, so that improved axial resolution is achieved solely by the illumination beams in very much the same way than in 2-photon excitation and in contrast to the regular confocal case. In FAM, however, it is not relevant whether point, line or other structured illumination patterns are used as long as activation and excitation are applied simultaneously, permitting the use of devices such as micro lens arrays, birefringent devices, SLMs, LCD or DMD projectors, LED arrays, holographic pattern generators etc, so FAM enables optical sectioning without necessarily requiring confocal optics or 2-photon excitation. Together with fluorescent proteins such as DRONPA fast 3d life cell imaging also becomes possible. Calculated point spread functions (PSF) suggest that this method may perfect other high resolution imaging techniques such as 4Pi, STED and PALM additionally by introducing an axial resolution in the 70nm range.

Further Information: PDF

DKFZ (German Cancer Research Center, Deutsches Krebsforschungszentrum)
Phone: +49-6221-42 2955

Contact
Dr. Ruth Herzog

info@technologieallianz.de | TechnologieAllianz e.V.
Further information:
http://www.technologieallianz.de/angebote.php?sort=sag&id=3231&lang=en
http://www.technologieallianz.de

More articles from Technology Offerings:

nachricht Flexible and rigid, heavy-metal free organic redox polymer batteries
09.03.2017 | TechnologieAllianz e.V.

nachricht Quat primer polymers – the universal key to permanent surface functionalization
27.02.2017 | TechnologieAllianz e.V.

All articles from Technology Offerings >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Giant Magnetic Fields in the Universe

Astronomers from Bonn and Tautenburg in Thuringia (Germany) used the 100-m radio telescope at Effelsberg to observe several galaxy clusters. At the edges of these large accumulations of dark matter, stellar systems (galaxies), hot gas, and charged particles, they found magnetic fields that are exceptionally ordered over distances of many million light years. This makes them the most extended magnetic fields in the universe known so far.

The results will be published on March 22 in the journal „Astronomy & Astrophysics“.

Galaxy clusters are the largest gravitationally bound structures in the universe. With a typical extent of about 10 million light years, i.e. 100 times the...

Im Focus: Tracing down linear ubiquitination

Researchers at the Goethe University Frankfurt, together with partners from the University of Tübingen in Germany and Queen Mary University as well as Francis Crick Institute from London (UK) have developed a novel technology to decipher the secret ubiquitin code.

Ubiquitin is a small protein that can be linked to other cellular proteins, thereby controlling and modulating their functions. The attachment occurs in many...

Im Focus: Perovskite edges can be tuned for optoelectronic performance

Layered 2D material improves efficiency for solar cells and LEDs

In the eternal search for next generation high-efficiency solar cells and LEDs, scientists at Los Alamos National Laboratory and their partners are creating...

Im Focus: Polymer-coated silicon nanosheets as alternative to graphene: A perfect team for nanoelectronics

Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are less stable. Now researchers at the Technical University of Munich (TUM) have, for the first time ever, produced a composite material combining silicon nanosheets and a polymer that is both UV-resistant and easy to process. This brings the scientists a significant step closer to industrial applications like flexible displays and photosensors.

Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are...

Im Focus: Researchers Imitate Molecular Crowding in Cells

Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to simulate these confined natural conditions in artificial vesicles for the first time. As reported in the academic journal Small, the results are offering better insight into the development of nanoreactors and artificial organelles.

Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

International Land Use Symposium ILUS 2017: Call for Abstracts and Registration open

20.03.2017 | Event News

CONNECT 2017: International congress on connective tissue

14.03.2017 | Event News

ICTM Conference: Turbine Construction between Big Data and Additive Manufacturing

07.03.2017 | Event News

 
Latest News

Pulverizing electronic waste is green, clean -- and cold

22.03.2017 | Materials Sciences

Astronomers hazard a ride in a 'drifting carousel' to understand pulsating stars

22.03.2017 | Physics and Astronomy

New gel-like coating beefs up the performance of lithium-sulfur batteries

22.03.2017 | Materials Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>