Based on high-level secondary research, current production systems allow for titers of ~106 – 108 functional virus particles per ml of lysate. Neverthe-less, optimization of virus yield may be of interest (i) for economic reasons and (ii) for larger Phase II / Phase III trials.<br><br> A novel possibility has been found to increase the yield of retroviruses or viral particles thereof from cells by APOBEC4 (Apolipoprotein B mRNA-editing catalytic polypeptide 4) or a modified APOBEC4. APOBEC4 is a human cellular gene belonging to the super family of RNA/DNA editing cytidine deaminases. It could be shown that the presence of N-terminal modified APOBEC4 may increase the yield of retro- or lentiviral vectors produced by a factor of up to 6. <br><br> Thus, these results position N-terminal modified stabilized APOBEC4 as a new agent to increase the production of retroviral vectors for gene therapy and offer a new interesting approach to identify drugs in a cell-based assay that may suppress HIV replication by suppressing APOBEC4. <br><br>
firstname.lastname@example.org | TechnologieAllianz e.V.
Autocorrelation-Based Detection of Inﬁnite Loops at Runtime
17.01.2017 | TechnologieAllianz e.V.
Phase-separation cell 1: Measurement equipment for liquid/liquid dispersion and separation processes in centrifugal field
13.01.2017 | TechnologieAllianz e.V.
Yersiniae cause severe intestinal infections. Studies using Yersinia pseudotuberculosis as a model organism aim to elucidate the infection mechanisms of these...
Researchers from the University of Hamburg in Germany, in collaboration with colleagues from the University of Aarhus in Denmark, have synthesized a new superconducting material by growing a few layers of an antiferromagnetic transition-metal chalcogenide on a bismuth-based topological insulator, both being non-superconducting materials.
While superconductivity and magnetism are generally believed to be mutually exclusive, surprisingly, in this new material, superconducting correlations...
Laser-driving of semimetals allows creating novel quasiparticle states within condensed matter systems and switching between different states on ultrafast time scales
Studying properties of fundamental particles in condensed matter systems is a promising approach to quantum field theory. Quasiparticles offer the opportunity...
Among the general public, solar thermal energy is currently associated with dark blue, rectangular collectors on building roofs. Technologies are needed for aesthetically high quality architecture which offer the architect more room for manoeuvre when it comes to low- and plus-energy buildings. With the “ArKol” project, researchers at Fraunhofer ISE together with partners are currently developing two façade collectors for solar thermal energy generation, which permit a high degree of design flexibility: a strip collector for opaque façade sections and a solar thermal blind for transparent sections. The current state of the two developments will be presented at the BAU 2017 trade fair.
As part of the “ArKol – development of architecturally highly integrated façade collectors with heat pipes” project, Fraunhofer ISE together with its partners...
At TU Wien, an alternative for resource intensive formwork for the construction of concrete domes was developed. It is now used in a test dome for the Austrian Federal Railways Infrastructure (ÖBB Infrastruktur).
Concrete shells are efficient structures, but not very resource efficient. The formwork for the construction of concrete domes alone requires a high amount of...
10.01.2017 | Event News
09.01.2017 | Event News
05.01.2017 | Event News
18.01.2017 | Power and Electrical Engineering
18.01.2017 | Materials Sciences
18.01.2017 | Life Sciences