Based on high-level secondary research, current production systems allow for titers of ~106 – 108 functional virus particles per ml of lysate. Neverthe-less, optimization of virus yield may be of interest (i) for economic reasons and (ii) for larger Phase II / Phase III trials.<br><br> A novel possibility has been found to increase the yield of retroviruses or viral particles thereof from cells by APOBEC4 (Apolipoprotein B mRNA-editing catalytic polypeptide 4) or a modified APOBEC4. APOBEC4 is a human cellular gene belonging to the super family of RNA/DNA editing cytidine deaminases. It could be shown that the presence of N-terminal modified APOBEC4 may increase the yield of retro- or lentiviral vectors produced by a factor of up to 6. <br><br> Thus, these results position N-terminal modified stabilized APOBEC4 as a new agent to increase the production of retroviral vectors for gene therapy and offer a new interesting approach to identify drugs in a cell-based assay that may suppress HIV replication by suppressing APOBEC4. <br><br>
firstname.lastname@example.org | TechnologieAllianz e.V.
Safety binding system for ski touring and telemarking
20.10.2016 | TechnologieAllianz e.V.
Enantiomerically pure chiral N-acyl-a-aminonitriles - Method for chemical production avoiding the need of toxic reagents
19.10.2016 | TechnologieAllianz e.V.
Ultrafast lasers have introduced new possibilities in engraving ultrafine structures, and scientists are now also investigating how to use them to etch microstructures into thin glass. There are possible applications in analytics (lab on a chip) and especially in electronics and the consumer sector, where great interest has been shown.
This new method was born of a surprising phenomenon: irradiating glass in a particular way with an ultrafast laser has the effect of making the glass up to a...
Terahertz excitation of selected crystal vibrations leads to an effective magnetic field that drives coherent spin motion
Controlling functional properties by light is one of the grand goals in modern condensed matter physics and materials science. A new study now demonstrates how...
Researchers from the Institute for Quantum Computing (IQC) at the University of Waterloo led the development of a new extensible wiring technique capable of controlling superconducting quantum bits, representing a significant step towards to the realization of a scalable quantum computer.
"The quantum socket is a wiring method that uses three-dimensional wires based on spring-loaded pins to address individual qubits," said Jeremy Béjanin, a PhD...
In a paper in Scientific Reports, a research team at Worcester Polytechnic Institute describes a novel light-activated phenomenon that could become the basis for applications as diverse as microscopic robotic grippers and more efficient solar cells.
A research team at Worcester Polytechnic Institute (WPI) has developed a revolutionary, light-activated semiconductor nanocomposite material that can be used...
By forcefully embedding two silicon atoms in a diamond matrix, Sandia researchers have demonstrated for the first time on a single chip all the components needed to create a quantum bridge to link quantum computers together.
"People have already built small quantum computers," says Sandia researcher Ryan Camacho. "Maybe the first useful one won't be a single giant quantum computer...
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14.10.2016 | Event News
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26.10.2016 | Physics and Astronomy
26.10.2016 | Earth Sciences
25.10.2016 | Earth Sciences