In FRET (Fluorescence Resonance Energy Transfer) experiments, dark quenchers like Dabcyl are widely used to avoid background noise resulting from an overlap between quencher and reporter fluorescence spectra. They provide a predictable way of modulating the fluorescence emission intensity of fluorophores without occupying an emission bandwidth. Although Dabcyl is one of the most frequently used fluorescence quencher, the very poor solubility in aqueous solutions due to its hydrophobic properties limits its use in biological systems where the natural solvent is water. The present invention provides a new dark quencher called Hydrodabcyl, which represents the hydrophilic alternative to Dabcyl.
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