Still, cells covered with HPL-medium do not experience optimal growth conditions, as they are usually cultured on a plastic surface which involves contact inhibition and enzymatic treatment during passaging. Invention :This invention relates to a cell culture system based upon human platelet lysate (HPL) and standard cell culture medium. The two-phase system consists of HPL-medium and HPL-gel. Both components only differ in the addition of a polymerization-inhibiting substance, e.g. heparin (compare Fig.1). In this way, growth factors are provided in a constant concentration throughout the culture system and three-dimensional cell growth is facilitated, thus minimizing contact inhibition. It has been shown that culturing mesenchymal stromal cells (MSC) on HPL-gel yields a higher proliferation rate (compare Fig. 3) and CFU-f outgrowth compared to a plastic surface and collagen-gel. Cell passaging can either be performed enzymatically or by mechanical gel fragmentation with a pipette (compare Fig. 2). Summing up, the novel HPL culture system provides excellent growth conditions for standard cell culture as well as for cell therapeutic applications.
firstname.lastname@example.org | TechnologieAllianz e.V.
Dosing system of bulk material (granulates) for expansion injection molding
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Efficient method for Chain Multiplication of unsaturated fatty acids - synthesis of ultra long-chain compounds
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For the first time ever, a cloud of ultra-cold atoms has been successfully created in space on board of a sounding rocket. The MAIUS mission demonstrates that quantum optical sensors can be operated even in harsh environments like space – a prerequi-site for finding answers to the most challenging questions of fundamental physics and an important innovation driver for everyday applications.
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Researchers from the University of Hamburg in Germany, in collaboration with colleagues from the University of Aarhus in Denmark, have synthesized a new superconducting material by growing a few layers of an antiferromagnetic transition-metal chalcogenide on a bismuth-based topological insulator, both being non-superconducting materials.
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Laser-driving of semimetals allows creating novel quasiparticle states within condensed matter systems and switching between different states on ultrafast time scales
Studying properties of fundamental particles in condensed matter systems is a promising approach to quantum field theory. Quasiparticles offer the opportunity...
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