Still, cells covered with HPL-medium do not experience optimal growth conditions, as they are usually cultured on a plastic surface which involves contact inhibition and enzymatic treatment during passaging. Invention :This invention relates to a cell culture system based upon human platelet lysate (HPL) and standard cell culture medium. The two-phase system consists of HPL-medium and HPL-gel. Both components only differ in the addition of a polymerization-inhibiting substance, e.g. heparin (compare Fig.1). In this way, growth factors are provided in a constant concentration throughout the culture system and three-dimensional cell growth is facilitated, thus minimizing contact inhibition. It has been shown that culturing mesenchymal stromal cells (MSC) on HPL-gel yields a higher proliferation rate (compare Fig. 3) and CFU-f outgrowth compared to a plastic surface and collagen-gel. Cell passaging can either be performed enzymatically or by mechanical gel fragmentation with a pipette (compare Fig. 2). Summing up, the novel HPL culture system provides excellent growth conditions for standard cell culture as well as for cell therapeutic applications.
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