Still, cells covered with HPL-medium do not experience optimal growth conditions, as they are usually cultured on a plastic surface which involves contact inhibition and enzymatic treatment during passaging. Invention :This invention relates to a cell culture system based upon human platelet lysate (HPL) and standard cell culture medium. The two-phase system consists of HPL-medium and HPL-gel. Both components only differ in the addition of a polymerization-inhibiting substance, e.g. heparin (compare Fig.1). In this way, growth factors are provided in a constant concentration throughout the culture system and three-dimensional cell growth is facilitated, thus minimizing contact inhibition. It has been shown that culturing mesenchymal stromal cells (MSC) on HPL-gel yields a higher proliferation rate (compare Fig. 3) and CFU-f outgrowth compared to a plastic surface and collagen-gel. Cell passaging can either be performed enzymatically or by mechanical gel fragmentation with a pipette (compare Fig. 2). Summing up, the novel HPL culture system provides excellent growth conditions for standard cell culture as well as for cell therapeutic applications.
firstname.lastname@example.org | TechnologieAllianz e.V.
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A newly developed laser technology has enabled physicists in the Laboratory for Attosecond Physics (jointly run by LMU Munich and the Max Planck Institute of Quantum Optics) to generate attosecond bursts of high-energy photons of unprecedented intensity. This has made it possible to observe the interaction of multiple photons in a single such pulse with electrons in the inner orbital shell of an atom.
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A group of researchers led by Andrea Cavalleri at the Max Planck Institute for Structure and Dynamics of Matter (MPSD) in Hamburg has demonstrated a new method enabling precise measurements of the interatomic forces that hold crystalline solids together. The paper Probing the Interatomic Potential of Solids by Strong-Field Nonlinear Phononics, published online in Nature, explains how a terahertz-frequency laser pulse can drive very large deformations of the crystal.
By measuring the highly unusual atomic trajectories under extreme electromagnetic transients, the MPSD group could reconstruct how rigid the atomic bonds are...
Quantum computers may one day solve algorithmic problems which even the biggest supercomputers today can’t manage. But how do you test a quantum computer to...
For the first time, a team of researchers at the Max-Planck Institute (MPI) for Polymer Research in Mainz, Germany, has succeeded in making an integrated circuit (IC) from just a monolayer of a semiconducting polymer via a bottom-up, self-assembly approach.
In the self-assembly process, the semiconducting polymer arranges itself into an ordered monolayer in a transistor. The transistors are binary switches used...
Breakthrough provides a new concept of the design of molecular motors, sensors and electricity generators at nanoscale
Researchers from the Institute of Organic Chemistry and Biochemistry of the CAS (IOCB Prague), Institute of Physics of the CAS (IP CAS) and Palacký University...
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