Novel genetic engineered yeast strains (Saccharomyces cerevisae) have been established that produce increased ethanol yield while simultaneously reduce the production of the unwanted by-product glycerol. It is a strong industrial interest to reduce the glycerol formation during glucose catabolism and thereby increasing ethanol yield, also because glycerol disturbs the distillation process. Past approaches to reduce glycerol formation based e.g. on the deletion of either one or the two genes gpd1 and gpd2 of glycerol-3-phosphate dehydrogenase (GPDH), which is the rate-controlling enzyme in the glycerol formation pathway of the yeast Saccharomyces cerevisae. These isoenzymes play also a crucial role in osmoregulation and redox balance. While single deletion of either gpd1 or gpd2 does not noticeble decrease glycerol production, the gpd1∆gpd2∆ double deletion strain produces no glycerol, however with the negative side effect that growth and ethanol production is abolished under anaerobic conditions and strongly reduced under aerobic conditions.<br><br> In the novel genetic engineering approaches, a) the Gpd1 enzyme activity is only partly reduced in a gpd2∆-deleted CEN-PK113 yeast strain background by replacing the strong natural gpd1 promotor by a weak TEF1 promotor mutant or b) both enzyme activities of GPD1 and GPD2 are partly reduced. The strains with reduced GPD1 and GPD2 activity show an increase in ethanol production by 2-5% and a reduction in glycerol formation by 61-88% compared to wild type and a slight better growth rate than the TEFmut:GPD1 gpd2∆-strain (ethanol increase: 6,3%; glycerol formation reduction by 64%).
The technology was developed at the Technische Universität Berlin.
Weitere Informationen: PDF
Tel.: +49 (0)30/2125-4820
Dr. Dirk Dantz
firstname.lastname@example.org | TechnologieAllianz e.V.
New Lithium Salts of Pentafluorophenylamide Anions as Electrolytes in Lithium Ionic Batteries
18.04.2017 | TechnologieAllianz e.V.
Gratings on glass surfaces
28.03.2017 | TechnologieAllianz e.V.
More and more automobile companies are focusing on body parts made of carbon fiber reinforced plastics (CFRP). However, manufacturing and repair costs must be further reduced in order to make CFRP more economical in use. Together with the Volkswagen AG and five other partners in the project HolQueSt 3D, the Laser Zentrum Hannover e.V. (LZH) has developed laser processes for the automatic trimming, drilling and repair of three-dimensional components.
Automated manufacturing processes are the basis for ultimately establishing the series production of CFRP components. In the project HolQueSt 3D, the LZH has...
Reflecting the structure of composites found in nature and the ancient world, researchers at the University of Illinois at Urbana-Champaign have synthesized thin carbon nanotube (CNT) textiles that exhibit both high electrical conductivity and a level of toughness that is about fifty times higher than copper films, currently used in electronics.
"The structural robustness of thin metal films has significant importance for the reliable operation of smart skin and flexible electronics including...
The nearby, giant radio galaxy M87 hosts a supermassive black hole (BH) and is well-known for its bright jet dominating the spectrum over ten orders of magnitude in frequency. Due to its proximity, jet prominence, and the large black hole mass, M87 is the best laboratory for investigating the formation, acceleration, and collimation of relativistic jets. A research team led by Silke Britzen from the Max Planck Institute for Radio Astronomy in Bonn, Germany, has found strong indication for turbulent processes connecting the accretion disk and the jet of that galaxy providing insights into the longstanding problem of the origin of astrophysical jets.
Supermassive black holes form some of the most enigmatic phenomena in astrophysics. Their enormous energy output is supposed to be generated by the...
The probability to find a certain number of photons inside a laser pulse usually corresponds to a classical distribution of independent events, the so-called...
Microprocessors based on atomically thin materials hold the promise of the evolution of traditional processors as well as new applications in the field of flexible electronics. Now, a TU Wien research team led by Thomas Müller has made a breakthrough in this field as part of an ongoing research project.
Two-dimensional materials, or 2D materials for short, are extremely versatile, although – or often more precisely because – they are made up of just one or a...
20.04.2017 | Event News
18.04.2017 | Event News
03.04.2017 | Event News
27.04.2017 | Life Sciences
27.04.2017 | Physics and Astronomy
27.04.2017 | Earth Sciences