Novel genetic engineered yeast strains (Saccharomyces cerevisae) have been established that produce increased ethanol yield while simultaneously reduce the production of the unwanted by-product glycerol. It is a strong industrial interest to reduce the glycerol formation during glucose catabolism and thereby increasing ethanol yield, also because glycerol disturbs the distillation process. Past approaches to reduce glycerol formation based e.g. on the deletion of either one or the two genes gpd1 and gpd2 of glycerol-3-phosphate dehydrogenase (GPDH), which is the rate-controlling enzyme in the glycerol formation pathway of the yeast Saccharomyces cerevisae. These isoenzymes play also a crucial role in osmoregulation and redox balance. While single deletion of either gpd1 or gpd2 does not noticeble decrease glycerol production, the gpd1∆gpd2∆ double deletion strain produces no glycerol, however with the negative side effect that growth and ethanol production is abolished under anaerobic conditions and strongly reduced under aerobic conditions.<br><br> In the novel genetic engineering approaches, a) the Gpd1 enzyme activity is only partly reduced in a gpd2∆-deleted CEN-PK113 yeast strain background by replacing the strong natural gpd1 promotor by a weak TEF1 promotor mutant or b) both enzyme activities of GPD1 and GPD2 are partly reduced. The strains with reduced GPD1 and GPD2 activity show an increase in ethanol production by 2-5% and a reduction in glycerol formation by 61-88% compared to wild type and a slight better growth rate than the TEFmut:GPD1 gpd2∆-strain (ethanol increase: 6,3%; glycerol formation reduction by 64%).
The technology was developed at the Technische Universität Berlin.
Weitere Informationen: PDF
Tel.: +49 (0)30/2125-4820
Dr. Dirk Dantz
email@example.com | TechnologieAllianz e.V.
New Lithium Salts of Pentafluorophenylamide Anions as Electrolytes in Lithium Ionic Batteries
18.04.2017 | TechnologieAllianz e.V.
Gratings on glass surfaces
28.03.2017 | TechnologieAllianz e.V.
In just a few weeks from now, the Chinese space station Tiangong-1 will re-enter the Earth's atmosphere where it will to a large extent burn up. It is possible that some debris will reach the Earth's surface. Tiangong-1 is orbiting the Earth uncontrolled at a speed of approx. 29,000 km/h.Currently the prognosis relating to the time of impact currently lies within a window of several days. The scientists at Fraunhofer FHR have already been monitoring Tiangong-1 for a number of weeks with their TIRA system, one of the most powerful space observation radars in the world, with a view to supporting the German Space Situational Awareness Center and the ESA with their re-entry forecasts.
Following the loss of radio contact with Tiangong-1 in 2016 and due to the low orbital height, it is now inevitable that the Chinese space station will...
Fraunhofer Institute for Organic Electronics, Electron Beam and Plasma Technology FEP, provider of research and development services for OLED lighting solutions, announces the founding of the “OLED Licht Forum” and presents latest OLED design and lighting solutions during light+building, from March 18th – 23rd, 2018 in Frankfurt a.M./Germany, at booth no. F91 in Hall 4.0.
They are united in their passion for OLED (organic light emitting diodes) lighting with all of its unique facets and application possibilities. Thus experts in...
A new scenario seeking to explain how Mars' putative oceans came and went over the last 4 billion years implies that the oceans formed several hundred million...
For the first time, an interdisciplinary team from the University of Basel has succeeded in integrating artificial organelles into the cells of live zebrafish embryos. This innovative approach using artificial organelles as cellular implants offers new potential in treating a range of diseases, as the authors report in an article published in Nature Communications.
In the cells of higher organisms, organelles such as the nucleus or mitochondria perform a range of complex functions necessary for life. In the networks of...
Animal photoreceptors capture light with photopigments. Researchers from the University of Göttingen have now discovered that these photopigments fulfill an...
19.03.2018 | Event News
16.03.2018 | Event News
13.03.2018 | Event News
21.03.2018 | Physics and Astronomy
21.03.2018 | Materials Sciences
21.03.2018 | Life Sciences