Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

EndoMark - Transient selectable marker system in plants

26.10.2010
Selection marker systems are indispensable tools in the production of genetically modified organisms (GMOs). Since the efficiency of plant transformation is rather low, there is a strong need for reliable markers that help to isolate the few successfully transformed plants. Most commonly used selection markers consist of bacterial genes that confer resistance against different types of antibiotics, e.g. kanamycin or hygromycin or against herbi-cides.

In addition the constitutive over-expression of a specific gene by placing it under the control of a constitutive ex-pressing promoter may lead to undesirable impacts on metabolism and pleiotropic effects in general. To solve these problems the inventors developed a new selection system based on a chimera of a germination dependent promoter and an endogenous plant resistance gene. The system allows highly specific expression of the resistance marker limited to the germination phase of oil seed plants. Thus it may contribute to improved safety and public acceptance of genetically modified plants like canola.

The over-expressed gene Uridine hydrolase 1 (URH1) encodes a component of the plant nucleotide degradation pathway which catalyses the hydrolase reaction of the pyridine nucleoside uridine into its base uracil and ribose. Moreover, plants over-expressing Urh1 show a higher resistance towards increasing concentrations of 5-fluorouridine (5-FD) allowing them to survive under condi-tions which are not permissive for wild-type plants. The combination of the germination phase specific, tran-siently active plant promoter and the cDNA of Urh1 en-ables an early mutant selection and minimises the occur-rence of pleiotropic effects.

Further Information: PDF

PROvendis GmbH
Phone: +49 (0)208/94105 10

Contact
Dipl.-Ing. Alfred Schillert

info@technologieallianz.de | TechnologieAllianz e.V.
Further information:
http://www.technologieallianz.de/angebote.php?sort=sag&id=2157&lang=en
http://www.technologieallianz.de

More articles from Technology Offerings:

nachricht Asian plant helps against the Ebola virus
20.02.2017 | TechnologieAllianz e.V.

nachricht Novel carbonization process of PAN-nanofiber mats with enhanced surface area and porosity
20.02.2017 | TechnologieAllianz e.V.

All articles from Technology Offerings >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Safe glide at total engine failure with ELA-inside

On January 15, 2009, Chesley B. Sullenberger was celebrated world-wide: after the two engines had failed due to bird strike, he and his flight crew succeeded after a glide flight with an Airbus A320 in ditching on the Hudson River. All 155 people on board were saved.

On January 15, 2009, Chesley B. Sullenberger was celebrated world-wide: after the two engines had failed due to bird strike, he and his flight crew succeeded...

Im Focus: Breakthrough with a chain of gold atoms

In the field of nanoscience, an international team of physicists with participants from Konstanz has achieved a breakthrough in understanding heat transport

In the field of nanoscience, an international team of physicists with participants from Konstanz has achieved a breakthrough in understanding heat transport

Im Focus: DNA repair: a new letter in the cell alphabet

Results reveal how discoveries may be hidden in scientific “blind spots”

Cells need to repair damaged DNA in our genes to prevent the development of cancer and other diseases. Our cells therefore activate and send “repair-proteins”...

Im Focus: Dresdner scientists print tomorrow’s world

The Fraunhofer IWS Dresden and Technische Universität Dresden inaugurated their jointly operated Center for Additive Manufacturing Dresden (AMCD) with a festive ceremony on February 7, 2017. Scientists from various disciplines perform research on materials, additive manufacturing processes and innovative technologies, which build up components in a layer by layer process. This technology opens up new horizons for component design and combinations of functions. For example during fabrication, electrical conductors and sensors are already able to be additively manufactured into components. They provide information about stress conditions of a product during operation.

The 3D-printing technology, or additive manufacturing as it is often called, has long made the step out of scientific research laboratories into industrial...

Im Focus: Mimicking nature's cellular architectures via 3-D printing

Research offers new level of control over the structure of 3-D printed materials

Nature does amazing things with limited design materials. Grass, for example, can support its own weight, resist strong wind loads, and recover after being...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Booth and panel discussion – The Lindau Nobel Laureate Meetings at the AAAS 2017 Annual Meeting

13.02.2017 | Event News

Complex Loading versus Hidden Reserves

10.02.2017 | Event News

International Conference on Crystal Growth in Freiburg

09.02.2017 | Event News

 
Latest News

New pop-up strategy inspired by cuts, not folds

27.02.2017 | Materials Sciences

Sandia uses confined nanoparticles to improve hydrogen storage materials performance

27.02.2017 | Interdisciplinary Research

Decoding the genome's cryptic language

27.02.2017 | Life Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>