Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

EndoMark - Transient selectable marker system in plants

26.10.2010
Selection marker systems are indispensable tools in the production of genetically modified organisms (GMOs). Since the efficiency of plant transformation is rather low, there is a strong need for reliable markers that help to isolate the few successfully transformed plants. Most commonly used selection markers consist of bacterial genes that confer resistance against different types of antibiotics, e.g. kanamycin or hygromycin or against herbi-cides.

In addition the constitutive over-expression of a specific gene by placing it under the control of a constitutive ex-pressing promoter may lead to undesirable impacts on metabolism and pleiotropic effects in general. To solve these problems the inventors developed a new selection system based on a chimera of a germination dependent promoter and an endogenous plant resistance gene. The system allows highly specific expression of the resistance marker limited to the germination phase of oil seed plants. Thus it may contribute to improved safety and public acceptance of genetically modified plants like canola.

The over-expressed gene Uridine hydrolase 1 (URH1) encodes a component of the plant nucleotide degradation pathway which catalyses the hydrolase reaction of the pyridine nucleoside uridine into its base uracil and ribose. Moreover, plants over-expressing Urh1 show a higher resistance towards increasing concentrations of 5-fluorouridine (5-FD) allowing them to survive under condi-tions which are not permissive for wild-type plants. The combination of the germination phase specific, tran-siently active plant promoter and the cDNA of Urh1 en-ables an early mutant selection and minimises the occur-rence of pleiotropic effects.

Further Information: PDF

PROvendis GmbH
Phone: +49 (0)208/94105 10

Contact
Dipl.-Ing. Alfred Schillert

info@technologieallianz.de | TechnologieAllianz e.V.
Further information:
http://www.technologieallianz.de/angebote.php?sort=sag&id=2157&lang=en
http://www.technologieallianz.de

All articles from Technology Offerings >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Scientists spin artificial silk from whey protein

X-ray study throws light on key process for production

A Swedish-German team of researchers has cleared up a key process for the artificial production of silk. With the help of the intense X-rays from DESY's...

Im Focus: Quantum optical sensor for the first time tested in space – with a laser system from Berlin

For the first time ever, a cloud of ultra-cold atoms has been successfully created in space on board of a sounding rocket. The MAIUS mission demonstrates that quantum optical sensors can be operated even in harsh environments like space – a prerequi-site for finding answers to the most challenging questions of fundamental physics and an important innovation driver for everyday applications.

According to Albert Einstein's Equivalence Principle, all bodies are accelerated at the same rate by the Earth's gravity, regardless of their properties. This...

Im Focus: Traffic jam in empty space

New success for Konstanz physicists in studying the quantum vacuum

An important step towards a completely new experimental access to quantum physics has been made at University of Konstanz. The team of scientists headed by...

Im Focus: How gut bacteria can make us ill

HZI researchers decipher infection mechanisms of Yersinia and immune responses of the host

Yersiniae cause severe intestinal infections. Studies using Yersinia pseudotuberculosis as a model organism aim to elucidate the infection mechanisms of these...

Im Focus: Interfacial Superconductivity: Magnetic and superconducting order revealed simultaneously

Researchers from the University of Hamburg in Germany, in collaboration with colleagues from the University of Aarhus in Denmark, have synthesized a new superconducting material by growing a few layers of an antiferromagnetic transition-metal chalcogenide on a bismuth-based topological insulator, both being non-superconducting materials.

While superconductivity and magnetism are generally believed to be mutually exclusive, surprisingly, in this new material, superconducting correlations...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Sustainable Water use in Agriculture in Eastern Europe and Central Asia

19.01.2017 | Event News

12V, 48V, high-voltage – trends in E/E automotive architecture

10.01.2017 | Event News

2nd Conference on Non-Textual Information on 10 and 11 May 2017 in Hannover

09.01.2017 | Event News

 
Latest News

Arctic melt ponds form when meltwater clogs ice pores

24.01.2017 | Earth Sciences

Synthetic nanoparticles achieve the complexity of protein molecules

24.01.2017 | Life Sciences

PPPL physicist uncovers clues to mechanism behind magnetic reconnection

24.01.2017 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>