Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:


ECO-PESED - Efficient and codon optimized protein expression, secretion and detection system for the green microalga Chlamydomon

The wide use of recombinant polypeptides is limited by still insufficient means of production, namely, their expression in host cells. The most often used bacterial host cells generally have the considerable drawback that polypeptides of eukaryotic origin are not folded correctly and that undesirable posttranslational modifications may occur. One of the most severe drawbacks is that the target polypeptide is typically located in the cytoplasm of the host cells and must be isolated from said cytoplasm in a laborious, time-consuming and expensive downstream-processing.

In view of the above, an expression system is needed that is usable in an eukaryotic cell extensively autologous with respect to sugars, amino acids and that is extensively independent of growth factors. It has been shown that plant cells, in particular microalgae cells, can be used for the production of recombinant proteins and overcome most of the above problems. Microalga cultures are comparably easy to handle and, in principle, scalable to large production scales. Furthermore, microalgae merely require a slightly salty aqueous environment, CO2 and light, can be supplemented by simple carbon sources, or a combination of these strategies to grow rapidly and produce the recombinant protein of interest. Therefore, the cultivation of microalgae is relatively inexpensive. These cultures can be extensively free of sugars and amino acids. The use of solar energy to produce biomass and recombinant protein production is an attractive aspect of these cells in light of the goal of sustainability. Unfortunately, the efficient production of recombinant proteins in microalgae is hampered because of the tightly regulated gene expression, preference for certain nucleic acid codon usage, and bias to its own promoters. The present invention allows to overcome these problems with highly efficient production and direct secretion of target polypeptides into the medium and in addition enables simultaneous monitoring of the polypeptide production without disturbing the expression system. This system has in principle a large range of applications and may be used due to the safe organism for researchers, pharmaceutical targets and food and feed producers.

Further Information: PDF

PROvendis GmbH
Phone: +49 (0)208/94105 10

Dipl.-Ing. Alfred Schillert | TechnologieAllianz e.V.
Further information:

More articles from Technology Offerings:

nachricht Extracellular production of designer HAAs - Production process for the preparation of designer HAAs
09.10.2015 | TechnologieAllianz e.V.

nachricht Improving immunogenicity of dendritic cells
08.10.2015 | TechnologieAllianz e.V.

All articles from Technology Offerings >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Reliable in-line inspections of high-strength automotive body parts within seconds

Nondestructive material testing (NDT) is a fast and effective way to analyze the quality of a product during the manufacturing process. Because defective materials can lead to malfunctioning finished products, NDT is an essential quality assurance measure, especially in the manufacture of safety-critical components such as automotive B-pillars. NDT examines the quality without damaging the component or modifying the surface of the material. At this year's Blechexpo trade fair in Stuttgart, Fraunhofer IZFP will have an exhibit that demonstrates the nondestructive testing of high-strength automotive body parts using 3MA. The measurement results are available in a matter of seconds.

To minimize vehicle weight and fuel consumption while providing the highest level of crash safety, automotive bodies are reinforced with elements made from...

Im Focus: Kick-off for a new era of precision astronomy

The MICADO camera, a first light instrument for the European Extremely Large Telescope (E-ELT), has entered a new phase in the project: by agreeing to a Memorandum of Understanding, the partners in Germany, France, the Netherlands, Austria, and Italy, have all confirmed their participation. Following this milestone, the project's transition into its preliminary design phase was approved at a kick-off meeting held in Vienna. Two weeks earlier, on September 18, the consortium and the European Southern Observatory (ESO), which is building the telescope, have signed the corresponding collaboration agreement.

As the first dedicated camera for the E-ELT, MICADO will equip the giant telescope with a capability for diffraction-limited imaging at near-infrared...

Im Focus: Locusts at the wheel: University of Graz investigates collision detector inspired by insect eyes

Self-driving cars will be on our streets in the foreseeable future. In Graz, research is currently dedicated to an innovative driver assistance system that takes over control if there is a danger of collision. It was nature that inspired Dr Manfred Hartbauer from the Institute of Zoology at the University of Graz: in dangerous traffic situations, migratory locusts react around ten times faster than humans. Working together with an interdisciplinary team, Hartbauer is investigating an affordable collision detector that is equipped with artificial locust eyes and can recognise potential crashes in time, during both day and night.

Inspired by insects

Im Focus: Physicists shrink particle accelerator

Prototype demonstrates feasibility of building terahertz accelerators

An interdisciplinary team of researchers has built the first prototype of a miniature particle accelerator that uses terahertz radiation instead of radio...

Im Focus: Simple detection of magnetic skyrmions

New physical effect: researchers discover a change of electrical resistance in magnetic whirls

At present, tiny magnetic whirls – so called skyrmions – are discussed as promising candidates for bits in future robust and compact data storage devices. At...

All Focus news of the innovation-report >>>



Event News

EHFG 2015: Securing healthcare and sustainably strengthening healthcare systems

01.10.2015 | Event News

Conference in Brussels: Tracking and Tracing the Smallest Marine Life Forms

30.09.2015 | Event News

World Alzheimer`s Day – Professor Willnow: Clearer Insights into the Development of the Disease

17.09.2015 | Event News

Latest News

Unexpected information about Earth's climate history from Yellow River sediment

09.10.2015 | Earth Sciences

Single atom alloy platinum-copper catalysts cut costs, boost green technology

09.10.2015 | Life Sciences

Indefatigable Hearing

09.10.2015 | Life Sciences

More VideoLinks >>>