In view of the above, an expression system is needed that is usable in an eukaryotic cell extensively autologous with respect to sugars, amino acids and that is extensively independent of growth factors. It has been shown that plant cells, in particular microalgae cells, can be used for the production of recombinant proteins and overcome most of the above problems.
Microalga cultures are comparably easy to handle and, in principle, scalable to large production scales. Furthermore, microalgae merely require a slightly salty aqueous environment, CO2 and light, can be supplemented by simple carbon sources, or a combination of these strategies to grow rapidly and produce the recombinant protein of interest. Therefore, the cultivation of microalgae is relatively inexpensive. These cultures can be extensively free of sugars and amino acids. The use of solar energy to produce biomass and recombinant protein production is an attractive aspect of these cells in light of the goal of sustainability.
Unfortunately, the efficient production of recombinant proteins in microalgae is hampered because of the tightly regulated gene expression, preference for certain nucleic acid codon usage, and bias to its own promoters.
The present invention allows to overcome these problems with highly efficient production and direct secretion of target polypeptides into the medium and in addition enables simultaneous monitoring of the polypeptide production without disturbing the expression system.
This system has in principle a large range of applications and may be used due to the safe organism for researchers, pharmaceutical targets and food and feed producers.
Further Information: PDF
Phone: +49 (0)208/94105 10
Dipl.-Ing. Alfred Schillert
firstname.lastname@example.org | TechnologieAllianz e.V.
Bioabbaubare Nano- und Mesopolymerpartikel zum Schutz des pulmonalen Surfactants der Lunge
24.05.2016 | TechnologieAllianz e.V.
Mutation Detection in ctDNA or CTC - Highly sensitive, fast and cost-effective detection of gene modifications and mutations in ctDNA or CTC
23.05.2016 | TechnologieAllianz e.V.
In the Beyond EUV project, the Fraunhofer Institutes for Laser Technology ILT in Aachen and for Applied Optics and Precision Engineering IOF in Jena are developing key technologies for the manufacture of a new generation of microchips using EUV radiation at a wavelength of 6.7 nm. The resulting structures are barely thicker than single atoms, and they make it possible to produce extremely integrated circuits for such items as wearables or mind-controlled prosthetic limbs.
In 1965 Gordon Moore formulated the law that came to be named after him, which states that the complexity of integrated circuits doubles every one to two...
Characterization of high-quality material reveals important details relevant to next generation nanoelectronic devices
Quantum mechanics is the field of physics governing the behavior of things on atomic scales, where things work very differently from our everyday world.
When current comes in discrete packages: Viennese scientists unravel the quantum properties of the carbon material graphene
In 2010 the Nobel Prize in physics was awarded for the discovery of the exceptional material graphene, which consists of a single layer of carbon atoms...
The trend-forward world of display technology relies on innovative materials and novel approaches to steadily advance the visual experience, for example through higher pixel densities, better contrast, larger formats or user-friendler design. Fraunhofer ISC’s newly developed materials for optics and electronics now broaden the application potential of next generation displays. Learn about lower cost-effective wet-chemical printing procedures and the new materials at the Fraunhofer ISC booth # 1021 in North Hall D during the SID International Symposium on Information Display held from 22 to 27 May 2016 at San Francisco’s Moscone Center.
Staphylococcus aureus usually is a formidable bacterial pathogen. Sometimes, however, weakened forms are found in the blood of patients. Researchers of the University of Würzburg have now identified one mutation responsible for that phenomenon.
Staphylococcus aureus is a bacterium that is frequently found on the human skin and in the nose where it usually behaves inconspicuously. However, once inside...
24.05.2016 | Event News
20.05.2016 | Event News
19.05.2016 | Event News
24.05.2016 | Earth Sciences
24.05.2016 | Information Technology
24.05.2016 | Materials Sciences