Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

ECO-PESED - Efficient and codon optimized protein expression, secretion and detection system for the green microalga Chlamydomon

13.02.2013
The wide use of recombinant polypeptides is limited by still insufficient means of production, namely, their expression in host cells. The most often used bacterial host cells generally have the considerable drawback that polypeptides of eukaryotic origin are not folded correctly and that undesirable posttranslational modifications may occur. One of the most severe drawbacks is that the target polypeptide is typically located in the cytoplasm of the host cells and must be isolated from said cytoplasm in a laborious, time-consuming and expensive downstream-processing.

In view of the above, an expression system is needed that is usable in an eukaryotic cell extensively autologous with respect to sugars, amino acids and that is extensively independent of growth factors. It has been shown that plant cells, in particular microalgae cells, can be used for the production of recombinant proteins and overcome most of the above problems. Microalga cultures are comparably easy to handle and, in principle, scalable to large production scales. Furthermore, microalgae merely require a slightly salty aqueous environment, CO2 and light, can be supplemented by simple carbon sources, or a combination of these strategies to grow rapidly and produce the recombinant protein of interest. Therefore, the cultivation of microalgae is relatively inexpensive. These cultures can be extensively free of sugars and amino acids. The use of solar energy to produce biomass and recombinant protein production is an attractive aspect of these cells in light of the goal of sustainability. Unfortunately, the efficient production of recombinant proteins in microalgae is hampered because of the tightly regulated gene expression, preference for certain nucleic acid codon usage, and bias to its own promoters. The present invention allows to overcome these problems with highly efficient production and direct secretion of target polypeptides into the medium and in addition enables simultaneous monitoring of the polypeptide production without disturbing the expression system. This system has in principle a large range of applications and may be used due to the safe organism for researchers, pharmaceutical targets and food and feed producers.

Further Information: PDF

PROvendis GmbH
Phone: +49 (0)208/94105 10

Contact
Dipl.-Ing. Alfred Schillert

info@technologieallianz.de | TechnologieAllianz e.V.
Further information:
http://www.technologieallianz.de/angebote.php?sort=sag&id=3036&lang=en
http://www.technologieallianz.de

More articles from Technology Offerings:

nachricht New Lithium Salts of Pentafluorophenylamide Anions as Electrolytes in Lithium Ionic Batteries
18.04.2017 | TechnologieAllianz e.V.

nachricht Gratings on glass surfaces
28.03.2017 | TechnologieAllianz e.V.

All articles from Technology Offerings >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Climate satellite: Tracking methane with robust laser technology

Heatwaves in the Arctic, longer periods of vegetation in Europe, severe floods in West Africa – starting in 2021, scientists want to explore the emissions of the greenhouse gas methane with the German-French satellite MERLIN. This is made possible by a new robust laser system of the Fraunhofer Institute for Laser Technology ILT in Aachen, which achieves unprecedented measurement accuracy.

Methane is primarily the result of the decomposition of organic matter. The gas has a 25 times greater warming potential than carbon dioxide, but is not as...

Im Focus: How protons move through a fuel cell

Hydrogen is regarded as the energy source of the future: It is produced with solar power and can be used to generate heat and electricity in fuel cells. Empa researchers have now succeeded in decoding the movement of hydrogen ions in crystals – a key step towards more efficient energy conversion in the hydrogen industry of tomorrow.

As charge carriers, electrons and ions play the leading role in electrochemical energy storage devices and converters such as batteries and fuel cells. Proton...

Im Focus: A unique data centre for cosmological simulations

Scientists from the Excellence Cluster Universe at the Ludwig-Maximilians-Universität Munich have establised "Cosmowebportal", a unique data centre for cosmological simulations located at the Leibniz Supercomputing Centre (LRZ) of the Bavarian Academy of Sciences. The complete results of a series of large hydrodynamical cosmological simulations are available, with data volumes typically exceeding several hundred terabytes. Scientists worldwide can interactively explore these complex simulations via a web interface and directly access the results.

With current telescopes, scientists can observe our Universe’s galaxies and galaxy clusters and their distribution along an invisible cosmic web. From the...

Im Focus: Scientists develop molecular thermometer for contactless measurement using infrared light

Temperature measurements possible even on the smallest scale / Molecular ruby for use in material sciences, biology, and medicine

Chemists at Johannes Gutenberg University Mainz (JGU) in cooperation with researchers of the German Federal Institute for Materials Research and Testing (BAM)...

Im Focus: Optoelectronic Inline Measurement – Accurate to the Nanometer

Germany counts high-precision manufacturing processes among its advantages as a location. It’s not just the aerospace and automotive industries that require almost waste-free, high-precision manufacturing to provide an efficient way of testing the shape and orientation tolerances of products. Since current inline measurement technology not yet provides the required accuracy, the Fraunhofer Institute for Laser Technology ILT is collaborating with four renowned industry partners in the INSPIRE project to develop inline sensors with a new accuracy class. Funded by the German Federal Ministry of Education and Research (BMBF), the project is scheduled to run until the end of 2019.

New Manufacturing Technologies for New Products

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Plants are networkers

19.06.2017 | Event News

Digital Survival Training for Executives

13.06.2017 | Event News

Global Learning Council Summit 2017

13.06.2017 | Event News

 
Latest News

A new technique isolates neuronal activity during memory consolidation

22.06.2017 | Life Sciences

Plant inspiration could lead to flexible electronics

22.06.2017 | Materials Sciences

A rhodium-based catalyst for making organosilicon using less precious metal

22.06.2017 | Materials Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>