LINE-1 (Long INterspersed Element-1) elements represent the major class of mammalian non-LTR (long terminal repeat) retrotransposons which are cov-ering 35 % of the human genome. LINE-1 is the only autonomous non-LTR retrotransposon in the human genome because it is coding for the protein machinery required for its mobilization. <br><br> <strong>Technology</strong><br> We offer a system which allows inducible, controlled and conditional expres-sion and retrotransposition of non-LTR retrotransposons, in particular mam-malian LINE-1 elements, in mammalian systems (cell culture and in vivo). The LINE-1 element is tagged with a reporter gene cassette and set under control of a tetracycline/doxycycline-inducible promoter. The modified LINE-1 elements can be used for an efficient insertional mutagenesis screen in both germ line and somatic cells. Gene trapping approaches based on controlled retrotransposition during defined time periods of the embryonic development can be used to identify cancer genes in specific tissues and cell types. Inducible modified LINE-1 elements which are tagged with a therapeutic gene can be used as a gene delivery system in gene therapeutic approaches.<br><br>
firstname.lastname@example.org | TechnologieAllianz e.V.
New Lithium Salts of Pentafluorophenylamide Anions as Electrolytes in Lithium Ionic Batteries
18.04.2017 | TechnologieAllianz e.V.
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28.03.2017 | TechnologieAllianz e.V.
Physicists at the University of Bonn have managed to create optical hollows and more complex patterns into which the light of a Bose-Einstein condensate flows. The creation of such highly low-loss structures for light is a prerequisite for complex light circuits, such as for quantum information processing for a new generation of computers. The researchers are now presenting their results in the journal Nature Photonics.
Light particles (photons) occur as tiny, indivisible portions. Many thousands of these light portions can be merged to form a single super-photon if they are...
For the first time, scientists have shown that circular RNA is linked to brain function. When a RNA molecule called Cdr1as was deleted from the genome of mice, the animals had problems filtering out unnecessary information – like patients suffering from neuropsychiatric disorders.
While hundreds of circular RNAs (circRNAs) are abundant in mammalian brains, one big question has remained unanswered: What are they actually good for? In the...
An experimental small satellite has successfully collected and delivered data on a key measurement for predicting changes in Earth's climate.
The Radiometer Assessment using Vertically Aligned Nanotubes (RAVAN) CubeSat was launched into low-Earth orbit on Nov. 11, 2016, in order to test new...
A study led by scientists of the Max Planck Institute for the Structure and Dynamics of Matter (MPSD) at the Center for Free-Electron Laser Science in Hamburg presents evidence of the coexistence of superconductivity and “charge-density-waves” in compounds of the poorly-studied family of bismuthates. This observation opens up new perspectives for a deeper understanding of the phenomenon of high-temperature superconductivity, a topic which is at the core of condensed matter research since more than 30 years. The paper by Nicoletti et al has been published in the PNAS.
Since the beginning of the 20th century, superconductivity had been observed in some metals at temperatures only a few degrees above the absolute zero (minus...
Researchers from the University of Miami (UM) Rosenstiel School of Marine and Atmospheric Science, the Italian Space Agency (ASI), and the Instituto Geofisico--Escuela Politecnica Nacional (IGEPN) of Ecuador, showed an increasing volcanic danger on Cotopaxi in Ecuador using a powerful technique known as Interferometric Synthetic Aperture Radar (InSAR).
The Andes region in which Cotopaxi volcano is located is known to contain some of the world's most serious volcanic hazard. A mid- to large-size eruption has...
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