Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:


Controlled Activation of LINE-1 Retrotransposons in Mammals


LINE-1 (Long INterspersed Element-1) elements represent the major class of mammalian non-LTR (long terminal repeat) retrotransposons which are cov-ering 35 % of the human genome. LINE-1 is the only autonomous non-LTR retrotransposon in the human genome because it is coding for the protein machinery required for its mobilization. <br><br> <strong>Technology</strong><br> We offer a system which allows inducible, controlled and conditional expres-sion and retrotransposition of non-LTR retrotransposons, in particular mam-malian LINE-1 elements, in mammalian systems (cell culture and in vivo). The LINE-1 element is tagged with a reporter gene cassette and set under control of a tetracycline/doxycycline-inducible promoter. The modified LINE-1 elements can be used for an efficient insertional mutagenesis screen in both germ line and somatic cells. Gene trapping approaches based on controlled retrotransposition during defined time periods of the embryonic development can be used to identify cancer genes in specific tissues and cell types. Inducible modified LINE-1 elements which are tagged with a therapeutic gene can be used as a gene delivery system in gene therapeutic approaches.<br><br>

<b>Benefits:</b><ul> <li>Induction of L1 retrotransposition for a defined period of time</li> <li>Tissue-specific L1 retrotransposition induction possible by using a vector-construct, coding for rtTA or tTA functionally linked to a tissue-specific promoter </li> <li>Proof of principle Tet/Dox – On/Off system shown in M2-HeLa cells</li></ul> <p><strong>IP Rights</strong><br> Patent granted in DE, GB, FR (EP 2205750), US patent pending (priority date 10/2007). <br><br> <strong>Patent Owner</strong><br> Paul-Ehrlich-Institut, Federal Agency for Sera and Vaccines, Langen

Further Information: PDF

ipal GmbH
Phone: +49 (0)30/2125-4820

Dr. Dirk Dantz | TechnologieAllianz e.V.
Further information:

More articles from Technology Offerings:

nachricht New Lithium Salts of Pentafluorophenylamide Anions as Electrolytes in Lithium Ionic Batteries
18.04.2017 | TechnologieAllianz e.V.

nachricht Gratings on glass surfaces
28.03.2017 | TechnologieAllianz e.V.

All articles from Technology Offerings >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Mars' oceans formed early, possibly aided by massive volcanic eruptions

Oceans formed before Tharsis and evolved together, shaping climate history of Mars

A new scenario seeking to explain how Mars' putative oceans came and went over the last 4 billion years implies that the oceans formed several hundred million...

Im Focus: Tiny implants for cells are functional in vivo

For the first time, an interdisciplinary team from the University of Basel has succeeded in integrating artificial organelles into the cells of live zebrafish embryos. This innovative approach using artificial organelles as cellular implants offers new potential in treating a range of diseases, as the authors report in an article published in Nature Communications.

In the cells of higher organisms, organelles such as the nucleus or mitochondria perform a range of complex functions necessary for life. In the networks of...

Im Focus: Locomotion control with photopigments

Researchers from Göttingen University discover additional function of opsins

Animal photoreceptors capture light with photopigments. Researchers from the University of Göttingen have now discovered that these photopigments fulfill an...

Im Focus: Surveying the Arctic: Tracking down carbon particles

Researchers embark on aerial campaign over Northeast Greenland

On 15 March, the AWI research aeroplane Polar 5 will depart for Greenland. Concentrating on the furthest northeast region of the island, an international team...

Im Focus: Unique Insights into the Antarctic Ice Shelf System

Data collected on ocean-ice interactions in the little-researched regions of the far south

The world’s second-largest ice shelf was the destination for a Polarstern expedition that ended in Punta Arenas, Chile on 14th March 2018. Oceanographers from...

All Focus news of the innovation-report >>>



Industry & Economy
Event News

Virtual reality conference comes to Reutlingen

19.03.2018 | Event News

Ultrafast Wireless and Chip Design at the DATE Conference in Dresden

16.03.2018 | Event News

International Tinnitus Conference of the Tinnitus Research Initiative in Regensburg

13.03.2018 | Event News

Latest News

Physicists made crystal lattice from polaritons

20.03.2018 | Physics and Astronomy

Mars' oceans formed early, possibly aided by massive volcanic eruptions

20.03.2018 | Physics and Astronomy

Thawing permafrost produces more methane than expected

20.03.2018 | Earth Sciences

Science & Research
Overview of more VideoLinks >>>