LINE-1 (Long INterspersed Element-1) elements represent the major class of mammalian non-LTR (long terminal repeat) retrotransposons which are cov-ering 35 % of the human genome. LINE-1 is the only autonomous non-LTR retrotransposon in the human genome because it is coding for the protein machinery required for its mobilization. <br><br> <strong>Technology</strong><br> We offer a system which allows inducible, controlled and conditional expres-sion and retrotransposition of non-LTR retrotransposons, in particular mam-malian LINE-1 elements, in mammalian systems (cell culture and in vivo). The LINE-1 element is tagged with a reporter gene cassette and set under control of a tetracycline/doxycycline-inducible promoter. The modified LINE-1 elements can be used for an efficient insertional mutagenesis screen in both germ line and somatic cells. Gene trapping approaches based on controlled retrotransposition during defined time periods of the embryonic development can be used to identify cancer genes in specific tissues and cell types. Inducible modified LINE-1 elements which are tagged with a therapeutic gene can be used as a gene delivery system in gene therapeutic approaches.<br><br>
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Quat primer polymers the universal key to permanent surface functionalization
27.02.2017 | TechnologieAllianz e.V.
Novel carbonization process of PAN-nanofiber mats with enhanced surface area and porosity
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On January 15, 2009, Chesley B. Sullenberger was celebrated world-wide: after the two engines had failed due to bird strike, he and his flight crew succeeded after a glide flight with an Airbus A320 in ditching on the Hudson River. All 155 people on board were saved.
On January 15, 2009, Chesley B. Sullenberger was celebrated world-wide: after the two engines had failed due to bird strike, he and his flight crew succeeded...
In the field of nanoscience, an international team of physicists with participants from Konstanz has achieved a breakthrough in understanding heat transport
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The Fraunhofer IWS Dresden and Technische Universität Dresden inaugurated their jointly operated Center for Additive Manufacturing Dresden (AMCD) with a festive ceremony on February 7, 2017. Scientists from various disciplines perform research on materials, additive manufacturing processes and innovative technologies, which build up components in a layer by layer process. This technology opens up new horizons for component design and combinations of functions. For example during fabrication, electrical conductors and sensors are already able to be additively manufactured into components. They provide information about stress conditions of a product during operation.
The 3D-printing technology, or additive manufacturing as it is often called, has long made the step out of scientific research laboratories into industrial...
Nature does amazing things with limited design materials. Grass, for example, can support its own weight, resist strong wind loads, and recover after being...
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