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re-CAT - Regeneration of cofactor in catalytic process by aldehyde dehydrogenase

22.01.2010
In biochemical processes, cofactors like NAD(P)H to NADP+ are commonly used as reduction agents. However, the regeneration of these valuable cofactors is generally cost-intensive.

Enzymatic regeneration of cofactor At the Rheinische Friedrich-Wilhelms-Universität Bonn, a novel class of aldehyde dehydrogenase for the regeneration of the cofactor NAD(P)H in biochemical reduction processes (re-CAT) has been developed. These new enzymes (derived from Gluconobacter oxydans) were successfully tested in a regio-selective reduction of α-ketocarbonyl compounds to α-hydroxy carbonyl compounds. Cost effective aldehydes, like the commodity chemical acetaldehyde, were employed as reduction agents.

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