Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:


New technology for bioseparation

Microfluidics and magnets replace pipettes and test tubes to simplify and improve diagnostics and detection of biomolecules

Separating target molecules in biological samples is a critical part of diagnosing and detecting diseases. Usually the target and probe molecules are mixed and then separated in batch processes that require multiple pipetting, tube washing and extraction steps that can affect accuracy.

This is an illustration showing a simple new technique that is capable of separating tiny amounts of the target molecules from mixed solutions.

Credit: J.Wang/Brown

Now a team of researchers at Brown University has developed a simple new technique that is capable of separating tiny amounts of the target molecules from mixed solutions by single motion of magnet under a microchannel. Their technique may make pipettes and test tubes a thing of the past in some diagnostic applications and increase the accuracy and sensitivity of disease detection.

The new platform developed by Anubhav Tripathi and his team at Brown doesn't rely on external pumps to mix samples or flow target molecules. Instead, their system is static and handy for researchers to use, according to Ms. Jingjing Wang, a graduate student pursuing her PhD. Bead-like magnetic particles are specifically modified by attaching short pieces of DNA to them that can capture target DNA molecules with specific sequences matching. Those are then separated for detection simply by pulling the magnetic beads along the channel. The process is simple, fast and specific.

This process has great applicability particularly for point-of-care platforms that are used to detect bacterial, viral infections and prion diseases by DNA, RNA or protein identification. Specific disease applications include testing for HIV and influenza, explained Wang.

"It can also be used to evaluate the expression of certain protein markers, such as troponin (an indicator of damage to the heart muscle) or any detection that requires binding and separation of known target biomolecules," she added.

Optimizing the system and characterizing the chip for biological assays was the biggest challenge for the research team as it required that both engineering as well as biological factors be considered, however the team is already developing assays using this new platform. A new microchip based Simple Method of Amplifying RNA Targets (SMART) assay developed to detect influenza from patient samples is already showing high agreement with Polymerase Chain Reaction (PCR), which is considered the "gold standard" for influenza diagnosis. The team's next challenge is developing assays using this technique to detect wild type and drug-resistant HIV in areas with limited resources such as Kenya and South Africa.

The article, "Microfluidic Platform for Isolating Nucleic Acid Targets Using Sequence Specific Hybridization" by Jingjing Wang, Kenneth Morabito, Jay X. Tang and Anubhav Tripathi appears in the journal Biomicrofluidics. See:


Biomicrofluidics publishes research highlighting fundamental physiochemical mechanisms associated with microfluidic and nanofluidic phenomena as well as novel microfluidic and nanofluidic techniques for diagnostic, medical, biological, pharmaceutical, environmental, and chemical applications. See:

Jason Socrates Bardi | EurekAlert!
Further information:

Further reports about: DNA DNA molecule HIV Little Brown Bats Target viral infection

More articles from Physics and Astronomy:

nachricht 'Frequency combs' ID chemicals within the mid-infrared spectral region
16.03.2018 | American Institute of Physics

nachricht Fraunhofer HHI have developed a novel single-polarization Kramers-Kronig receiver scheme
16.03.2018 | Fraunhofer-Institut für Nachrichtentechnik, Heinrich-Hertz-Institut, HHI

All articles from Physics and Astronomy >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Locomotion control with photopigments

Researchers from Göttingen University discover additional function of opsins

Animal photoreceptors capture light with photopigments. Researchers from the University of Göttingen have now discovered that these photopigments fulfill an...

Im Focus: Surveying the Arctic: Tracking down carbon particles

Researchers embark on aerial campaign over Northeast Greenland

On 15 March, the AWI research aeroplane Polar 5 will depart for Greenland. Concentrating on the furthest northeast region of the island, an international team...

Im Focus: Unique Insights into the Antarctic Ice Shelf System

Data collected on ocean-ice interactions in the little-researched regions of the far south

The world’s second-largest ice shelf was the destination for a Polarstern expedition that ended in Punta Arenas, Chile on 14th March 2018. Oceanographers from...

Im Focus: ILA 2018: Laser alternative to hexavalent chromium coating

At the 2018 ILA Berlin Air Show from April 25–29, the Fraunhofer Institute for Laser Technology ILT is showcasing extreme high-speed Laser Material Deposition (EHLA): A video documents how for metal components that are highly loaded, EHLA has already proved itself as an alternative to hard chrome plating, which is now allowed only under special conditions.

When the EU restricted the use of hexavalent chromium compounds to special applications requiring authorization, the move prompted a rethink in the surface...

Im Focus: Radar for navigation support from autonomous flying drones

At the ILA Berlin, hall 4, booth 202, Fraunhofer FHR will present two radar sensors for navigation support of drones. The sensors are valuable components in the implementation of autonomous flying drones: they function as obstacle detectors to prevent collisions. Radar sensors also operate reliably in restricted visibility, e.g. in foggy or dusty conditions. Due to their ability to measure distances with high precision, the radar sensors can also be used as altimeters when other sources of information such as barometers or GPS are not available or cannot operate optimally.

Drones play an increasingly important role in the area of logistics and services. Well-known logistic companies place great hope in these compact, aerial...

All Focus news of the innovation-report >>>



Industry & Economy
Event News

Ultrafast Wireless and Chip Design at the DATE Conference in Dresden

16.03.2018 | Event News

International Tinnitus Conference of the Tinnitus Research Initiative in Regensburg

13.03.2018 | Event News

International Virtual Reality Conference “IEEE VR 2018” comes to Reutlingen, Germany

08.03.2018 | Event News

Latest News

Wandering greenhouse gas

16.03.2018 | Earth Sciences

'Frequency combs' ID chemicals within the mid-infrared spectral region

16.03.2018 | Physics and Astronomy

Biologists unravel another mystery of what makes DNA go 'loopy'

16.03.2018 | Life Sciences

Science & Research
Overview of more VideoLinks >>>