Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

New technology for bioseparation

18.09.2013
Microfluidics and magnets replace pipettes and test tubes to simplify and improve diagnostics and detection of biomolecules

Separating target molecules in biological samples is a critical part of diagnosing and detecting diseases. Usually the target and probe molecules are mixed and then separated in batch processes that require multiple pipetting, tube washing and extraction steps that can affect accuracy.


This is an illustration showing a simple new technique that is capable of separating tiny amounts of the target molecules from mixed solutions.

Credit: J.Wang/Brown

Now a team of researchers at Brown University has developed a simple new technique that is capable of separating tiny amounts of the target molecules from mixed solutions by single motion of magnet under a microchannel. Their technique may make pipettes and test tubes a thing of the past in some diagnostic applications and increase the accuracy and sensitivity of disease detection.

The new platform developed by Anubhav Tripathi and his team at Brown doesn't rely on external pumps to mix samples or flow target molecules. Instead, their system is static and handy for researchers to use, according to Ms. Jingjing Wang, a graduate student pursuing her PhD. Bead-like magnetic particles are specifically modified by attaching short pieces of DNA to them that can capture target DNA molecules with specific sequences matching. Those are then separated for detection simply by pulling the magnetic beads along the channel. The process is simple, fast and specific.

This process has great applicability particularly for point-of-care platforms that are used to detect bacterial, viral infections and prion diseases by DNA, RNA or protein identification. Specific disease applications include testing for HIV and influenza, explained Wang.

"It can also be used to evaluate the expression of certain protein markers, such as troponin (an indicator of damage to the heart muscle) or any detection that requires binding and separation of known target biomolecules," she added.

Optimizing the system and characterizing the chip for biological assays was the biggest challenge for the research team as it required that both engineering as well as biological factors be considered, however the team is already developing assays using this new platform. A new microchip based Simple Method of Amplifying RNA Targets (SMART) assay developed to detect influenza from patient samples is already showing high agreement with Polymerase Chain Reaction (PCR), which is considered the "gold standard" for influenza diagnosis. The team's next challenge is developing assays using this technique to detect wild type and drug-resistant HIV in areas with limited resources such as Kenya and South Africa.

The article, "Microfluidic Platform for Isolating Nucleic Acid Targets Using Sequence Specific Hybridization" by Jingjing Wang, Kenneth Morabito, Jay X. Tang and Anubhav Tripathi appears in the journal Biomicrofluidics. See: http://dx.doi.org/10.1063/1.4816943

ABOUT THE JOURNAL

Biomicrofluidics publishes research highlighting fundamental physiochemical mechanisms associated with microfluidic and nanofluidic phenomena as well as novel microfluidic and nanofluidic techniques for diagnostic, medical, biological, pharmaceutical, environmental, and chemical applications. See: http://bmf.aip.org

Jason Socrates Bardi | EurekAlert!
Further information:
http://www.aip.org

Further reports about: DNA DNA molecule HIV Little Brown Bats Target viral infection

More articles from Physics and Astronomy:

nachricht A better way to weigh millions of solitary stars
15.12.2017 | Vanderbilt University

nachricht A chip for environmental and health monitoring
15.12.2017 | Friedrich-Alexander-Universität Erlangen-Nürnberg

All articles from Physics and Astronomy >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: First-of-its-kind chemical oscillator offers new level of molecular control

DNA molecules that follow specific instructions could offer more precise molecular control of synthetic chemical systems, a discovery that opens the door for engineers to create molecular machines with new and complex behaviors.

Researchers have created chemical amplifiers and a chemical oscillator using a systematic method that has the potential to embed sophisticated circuit...

Im Focus: Long-lived storage of a photonic qubit for worldwide teleportation

MPQ scientists achieve long storage times for photonic quantum bits which break the lower bound for direct teleportation in a global quantum network.

Concerning the development of quantum memories for the realization of global quantum networks, scientists of the Quantum Dynamics Division led by Professor...

Im Focus: Electromagnetic water cloak eliminates drag and wake

Detailed calculations show water cloaks are feasible with today's technology

Researchers have developed a water cloaking concept based on electromagnetic forces that could eliminate an object's wake, greatly reducing its drag while...

Im Focus: Scientists channel graphene to understand filtration and ion transport into cells

Tiny pores at a cell's entryway act as miniature bouncers, letting in some electrically charged atoms--ions--but blocking others. Operating as exquisitely sensitive filters, these "ion channels" play a critical role in biological functions such as muscle contraction and the firing of brain cells.

To rapidly transport the right ions through the cell membrane, the tiny channels rely on a complex interplay between the ions and surrounding molecules,...

Im Focus: Towards data storage at the single molecule level

The miniaturization of the current technology of storage media is hindered by fundamental limits of quantum mechanics. A new approach consists in using so-called spin-crossover molecules as the smallest possible storage unit. Similar to normal hard drives, these special molecules can save information via their magnetic state. A research team from Kiel University has now managed to successfully place a new class of spin-crossover molecules onto a surface and to improve the molecule’s storage capacity. The storage density of conventional hard drives could therefore theoretically be increased by more than one hundred fold. The study has been published in the scientific journal Nano Letters.

Over the past few years, the building blocks of storage media have gotten ever smaller. But further miniaturization of the current technology is hindered by...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

See, understand and experience the work of the future

11.12.2017 | Event News

Innovative strategies to tackle parasitic worms

08.12.2017 | Event News

AKL’18: The opportunities and challenges of digitalization in the laser industry

07.12.2017 | Event News

 
Latest News

Engineers program tiny robots to move, think like insects

15.12.2017 | Power and Electrical Engineering

One in 5 materials chemistry papers may be wrong, study suggests

15.12.2017 | Materials Sciences

New antbird species discovered in Peru by LSU ornithologists

15.12.2017 | Life Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>