Health professionals send genes and healthy cells on their way through the bloodstream so that they can, for example, repair tissue damage to arteries. But do they reach their destination in sufficient quantities?
Scientists of the PTB have developed a highly sensitive measuring method with which the efficiency of this therapy can be investigated: Small magnetic particles which are situated on the planted gene or on the planted cell can with the aid of an external magnetic field be specifically directed to the location of the damage.
There the researchers determine, accurate to the picogram per cell, the quantity of the magnetic material – and thus also the quantity of the therapeutically effective genes or cells. In a joint study with the University of Bonn it became clear: By means of the magnetic method it is possible to dramatically increase the efficiency of the gene transfer in comparison to the non-magnetic method.
Magnetic nanoparticles can support or even enable gene transfer under clinically relevant experimental conditions. For the transduction of human cells, gene carriers were coupled to magnetic nanoparticles and dragged into the cells by magnetic field gradients. The efficiency of magnetic transduction turned out to be much higher than the nonmagnetic procedure. An additional welcome side effect is the "magnetization" of the cells after the incorporation of nanoparticles. This may enable the targeted transport of the cells to regions of interest.
A closer look at the underlying mechanism of magnetic gene transfer was taken by the quantification of the magnetic material that was delivered to the cells. The required highly sensitive measurements in the range of a few picogramm per cell were made by PTB using magnetorelaxometry. The good correlation between measurement data and gene transfer encourages to use magnetorelaxometry for monitoring the efficiency of gene and cell transfer, possibly even in vivo.
Dr. Lutz Trahms | EurekAlert!
APEX takes a glimpse into the heart of darkness
25.05.2018 | Max-Planck-Institut für Radioastronomie
First chip-scale broadband optical system that can sense molecules in the mid-IR
24.05.2018 | Columbia University School of Engineering and Applied Science
The more electronics steer, accelerate and brake cars, the more important it is to protect them against cyber-attacks. That is why 15 partners from industry and academia will work together over the next three years on new approaches to IT security in self-driving cars. The joint project goes by the name Security For Connected, Autonomous Cars (SecForCARs) and has funding of €7.2 million from the German Federal Ministry of Education and Research. Infineon is leading the project.
Vehicles already offer diverse communication interfaces and more and more automated functions, such as distance and lane-keeping assist systems. At the same...
A research team led by physicists at the Technical University of Munich (TUM) has developed molecular nanoswitches that can be toggled between two structurally different states using an applied voltage. They can serve as the basis for a pioneering class of devices that could replace silicon-based components with organic molecules.
The development of new electronic technologies drives the incessant reduction of functional component sizes. In the context of an international collaborative...
At the LASYS 2018, from June 5th to 7th, the Laser Zentrum Hannover e.V. (LZH) will be showcasing processes for the laser material processing of tomorrow in hall 4 at stand 4E75. With blown bomb shells the LZH will present first results of a research project on civil security.
At this year's LASYS, the LZH will exhibit light-based processes such as cutting, welding, ablation and structuring as well as additive manufacturing for...
There are videos on the internet that can make one marvel at technology. For example, a smartphone is casually bent around the arm or a thin-film display is rolled in all directions and with almost every diameter. From the user's point of view, this looks fantastic. From a professional point of view, however, the question arises: Is that already possible?
At Display Week 2018, scientists from the Fraunhofer Institute for Applied Polymer Research IAP will be demonstrating today’s technological possibilities and...
So-called quantum many-body scars allow quantum systems to stay out of equilibrium much longer, explaining experiment | Study published in Nature Physics
Recently, researchers from Harvard and MIT succeeded in trapping a record 53 atoms and individually controlling their quantum state, realizing what is called a...
25.05.2018 | Event News
02.05.2018 | Event News
13.04.2018 | Event News
25.05.2018 | Event News
25.05.2018 | Machine Engineering
25.05.2018 | Life Sciences