Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

German scientists advance light microscopy

21.10.2008
Although more than 400 years old, optical microscopy is still one of the most important methods used in medical research and biology.

Its potential, however, could still increase significantly due to an invention recently made by a team of interdisciplinary working physicists.

Published as cover story in the current issue of the open access Journal "Optics Express", the researchers from Prof. Käs' group in Leipzig, in collaboration with researchers in Jena and Cambridge present the Optical Cell Rotator (OCR), a novel tool for the contact-free orientation of biological samples using laser radiation.

According to first author Moritz Kreysing the new technology could be the missing key to allow for the realization of single-cell optical tomography, which has been shown in principle but not in any useful implementation.

In contrast to the current standards for 3D imaging of cells, namely confocal and deconvolution microscopy, tomographic imaging provides maximum resolution in all three dimensions. To achieve this feat specimens are rotated stepwise and imaged from multiple angles. Using this data a computer is able to reconstruct a three-dimensional model of the sample containing 2.5 times more information than traditionally accessible.

The Optical Cell Rotator is of fundamental importance in this context for the integrity of living samples. While the orientation of cells previously required their exposure to either strong electric fields or unphysiological mechanical tools, the new device promises a much more gentle handling of the sensitive material. Thus, OCR is probably most useful for the investigation of stem cells, which are notoriously sensitive to handling.

http://www.opticsinfobase.org/oe/viewmedia.cfm?uri=oe-16-21-16984&seq=0
Abstract: http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-16-21-16984
On the cover: http://www.opticsinfobase.org/oe/issue.cfm?volume=16&issue=21
Publication supplements:
1: http://www.opticsinfobase.org/oe/viewmedia.cfm?uri=oe-16-21-16984&seq=1
2: http://www.opticsinfobase.org/oe/viewmedia.cfm?uri=oe-16-21-16984&seq=2
Weitere Informationen:
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-16-21-16984
http://www.opticsinfobase.org/oe/issue.cfm?volume=16&issue=21

Dr. Bärbel Adams | idw
Further information:
http://www.uni-leipzig.de

All articles from Physics and Astronomy >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: LaserTAB: More efficient and precise contacts thanks to human-robot collaboration

At the productronica trade fair in Munich this November, the Fraunhofer Institute for Laser Technology ILT will be presenting Laser-Based Tape-Automated Bonding, LaserTAB for short. The experts from Aachen will be demonstrating how new battery cells and power electronics can be micro-welded more efficiently and precisely than ever before thanks to new optics and robot support.

Fraunhofer ILT from Aachen relies on a clever combination of robotics and a laser scanner with new optics as well as process monitoring, which it has developed...

Im Focus: The pyrenoid is a carbon-fixing liquid droplet

Plants and algae use the enzyme Rubisco to fix carbon dioxide, removing it from the atmosphere and converting it into biomass. Algae have figured out a way to increase the efficiency of carbon fixation. They gather most of their Rubisco into a ball-shaped microcompartment called the pyrenoid, which they flood with a high local concentration of carbon dioxide. A team of scientists at Princeton University, the Carnegie Institution for Science, Stanford University and the Max Plank Institute of Biochemistry have unravelled the mysteries of how the pyrenoid is assembled. These insights can help to engineer crops that remove more carbon dioxide from the atmosphere while producing more food.

A warming planet

Im Focus: Highly precise wiring in the Cerebral Cortex

Our brains house extremely complex neuronal circuits, whose detailed structures are still largely unknown. This is especially true for the so-called cerebral cortex of mammals, where among other things vision, thoughts or spatial orientation are being computed. Here the rules by which nerve cells are connected to each other are only partly understood. A team of scientists around Moritz Helmstaedter at the Frankfiurt Max Planck Institute for Brain Research and Helene Schmidt (Humboldt University in Berlin) have now discovered a surprisingly precise nerve cell connectivity pattern in the part of the cerebral cortex that is responsible for orienting the individual animal or human in space.

The researchers report online in Nature (Schmidt et al., 2017. Axonal synapse sorting in medial entorhinal cortex, DOI: 10.1038/nature24005) that synapses in...

Im Focus: Tiny lasers from a gallery of whispers

New technique promises tunable laser devices

Whispering gallery mode (WGM) resonators are used to make tiny micro-lasers, sensors, switches, routers and other devices. These tiny structures rely on a...

Im Focus: Ultrafast snapshots of relaxing electrons in solids

Using ultrafast flashes of laser and x-ray radiation, scientists at the Max Planck Institute of Quantum Optics (Garching, Germany) took snapshots of the briefest electron motion inside a solid material to date. The electron motion lasted only 750 billionths of the billionth of a second before it fainted, setting a new record of human capability to capture ultrafast processes inside solids!

When x-rays shine onto solid materials or large molecules, an electron is pushed away from its original place near the nucleus of the atom, leaving a hole...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

“Lasers in Composites Symposium” in Aachen – from Science to Application

19.09.2017 | Event News

I-ESA 2018 – Call for Papers

12.09.2017 | Event News

EMBO at Basel Life, a new conference on current and emerging life science research

06.09.2017 | Event News

 
Latest News

Fraunhofer ISE Pushes World Record for Multicrystalline Silicon Solar Cells to 22.3 Percent

25.09.2017 | Power and Electrical Engineering

Usher syndrome: Gene therapy restores hearing and balance

25.09.2017 | Health and Medicine

An international team of physicists a coherent amplification effect in laser excited dielectrics

25.09.2017 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>