Researchers at the University of Illinois at Urbana-Champaign have introduced a new class of light-emitting quantum dots (QDs) with tunable and equalized fluorescence brightness across a broad range of colors. This results in more accurate measurements of molecules in diseased tissue and improved quantitative imaging capabilities.
"In this work, we have made two major advances--the ability to precisely control the brightness of light-emitting particles called quantum dots, and the ability to make multiple colors equal in brightness," explained Andrew M. Smith, an assistant professor of bioengineering at Illinois.
Left: Conventional fluorescent materials like quantum dots and dyes have mismatched brightness between different colors. When these materials are administered to a tumor (shown below) to measure molecular concentrations, the signals are dominated by the brighter fluorophores. Right: New brightness-equalized quantum dots that have equal fluorescence brightness for different colors. When these are administered to tumors, the signals are evenly matched, allowing measurement of many molecules at the same time.
Credit: University of Illinois
"Previously light emission had an unknown correspondence with molecule number. Now it can be precisely tuned and calibrated to accurately count specific molecules. This will be particularly useful for understanding complex processes in neurons and cancer cells to help us unravel disease mechanisms, and for characterizing cells from diseased tissue of patients."
"Fluorescent dyes have been used to label molecules in cells and tissues for nearly a century, and have molded our understanding of cellular structures and protein function. But it has always been challenging to extract quantitative information because the amount of light emitted from a single dye is unstable and often unpredictable.
Also the brightness varies drastically between different colors, which complicates the use of multiple dye colors at the same time. These attributes obscure correlations between measured light intensity and concentrations of molecules," stated Sung Jun Lim, a postdoctoral fellow and first author of the paper, "Brightness-Equalized Quantum Dots," published this week in Nature Communications.
According to the researchers, these new materials will be especially important for imaging in complex tissues and living organisms where there is a major need for quantitative imaging tools, and can provide a consistent and tunable number of photons per tagged biomolecule.
They are also expected to be used for precise color matching in light-emitting devices and displays, and for photon-on-demand encryption applications. The same principles should be applicable across a wide range of semiconducting materials.
"The capacity to independently tune the QD fluorescence brightness and color has never before been possible, and these BE-QDs now provide this capability," said Lim. "We have developed new materials-engineering principles that we anticipate will provide a diverse range of new optical capabilities, allow quantitative multicolor imaging in biological tissue, and improve color tuning in light-emitting devices.
In addition, BE-QDs maintain their equal brightness over time while whereas conventional QDs with mismatched brightness become further mismatched over time. These attributes should lead to new LEDs and display devices not only with precisely matched colors--better color accuracy and brightness--but also with improved performance lifetime and improved ease of manufacturing." QDs are already in use in display devices (e.g. Amazon Kindle and a new Samsung TV).
In addition to Lim and Smith, co-authors include Mohammad U. Zahid, Phuong Le, Liang Ma, Bioengineering at Illinois; David Entenberg, Allison S. Harney, and John Condeelis, Albert Einstein College of Medicine, New York.
Andrew M. Smith | EurekAlert!
From rocks in Colorado, evidence of a 'chaotic solar system'
23.02.2017 | University of Wisconsin-Madison
Prediction: More gas-giants will be found orbiting Sun-like stars
22.02.2017 | Carnegie Institution for Science
Cells need to repair damaged DNA in our genes to prevent the development of cancer and other diseases. Our cells therefore activate and send “repair-proteins”...
The Fraunhofer IWS Dresden and Technische Universität Dresden inaugurated their jointly operated Center for Additive Manufacturing Dresden (AMCD) with a festive ceremony on February 7, 2017. Scientists from various disciplines perform research on materials, additive manufacturing processes and innovative technologies, which build up components in a layer by layer process. This technology opens up new horizons for component design and combinations of functions. For example during fabrication, electrical conductors and sensors are already able to be additively manufactured into components. They provide information about stress conditions of a product during operation.
The 3D-printing technology, or additive manufacturing as it is often called, has long made the step out of scientific research laboratories into industrial...
Nature does amazing things with limited design materials. Grass, for example, can support its own weight, resist strong wind loads, and recover after being...
Nanometer-scale magnetic perforated grids could create new possibilities for computing. Together with international colleagues, scientists from the Helmholtz Zentrum Dresden-Rossendorf (HZDR) have shown how a cobalt grid can be reliably programmed at room temperature. In addition they discovered that for every hole ("antidot") three magnetic states can be configured. The results have been published in the journal "Scientific Reports".
Physicist Dr. Rantej Bali from the HZDR, together with scientists from Singapore and Australia, designed a special grid structure in a thin layer of cobalt in...
13.02.2017 | Event News
10.02.2017 | Event News
09.02.2017 | Event News
24.02.2017 | Life Sciences
24.02.2017 | Life Sciences
24.02.2017 | Trade Fair News