Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Brightness-equalized quantum dots improve biological imaging

05.10.2015

Researchers at the University of Illinois at Urbana-Champaign have introduced a new class of light-emitting quantum dots (QDs) with tunable and equalized fluorescence brightness across a broad range of colors. This results in more accurate measurements of molecules in diseased tissue and improved quantitative imaging capabilities.

"In this work, we have made two major advances--the ability to precisely control the brightness of light-emitting particles called quantum dots, and the ability to make multiple colors equal in brightness," explained Andrew M. Smith, an assistant professor of bioengineering at Illinois.


Left: Conventional fluorescent materials like quantum dots and dyes have mismatched brightness between different colors. When these materials are administered to a tumor (shown below) to measure molecular concentrations, the signals are dominated by the brighter fluorophores. Right: New brightness-equalized quantum dots that have equal fluorescence brightness for different colors. When these are administered to tumors, the signals are evenly matched, allowing measurement of many molecules at the same time.

Credit: University of Illinois

"Previously light emission had an unknown correspondence with molecule number. Now it can be precisely tuned and calibrated to accurately count specific molecules. This will be particularly useful for understanding complex processes in neurons and cancer cells to help us unravel disease mechanisms, and for characterizing cells from diseased tissue of patients."

"Fluorescent dyes have been used to label molecules in cells and tissues for nearly a century, and have molded our understanding of cellular structures and protein function. But it has always been challenging to extract quantitative information because the amount of light emitted from a single dye is unstable and often unpredictable.

Also the brightness varies drastically between different colors, which complicates the use of multiple dye colors at the same time. These attributes obscure correlations between measured light intensity and concentrations of molecules," stated Sung Jun Lim, a postdoctoral fellow and first author of the paper, "Brightness-Equalized Quantum Dots," published this week in Nature Communications.

According to the researchers, these new materials will be especially important for imaging in complex tissues and living organisms where there is a major need for quantitative imaging tools, and can provide a consistent and tunable number of photons per tagged biomolecule.

They are also expected to be used for precise color matching in light-emitting devices and displays, and for photon-on-demand encryption applications. The same principles should be applicable across a wide range of semiconducting materials.

"The capacity to independently tune the QD fluorescence brightness and color has never before been possible, and these BE-QDs now provide this capability," said Lim. "We have developed new materials-engineering principles that we anticipate will provide a diverse range of new optical capabilities, allow quantitative multicolor imaging in biological tissue, and improve color tuning in light-emitting devices.

In addition, BE-QDs maintain their equal brightness over time while whereas conventional QDs with mismatched brightness become further mismatched over time. These attributes should lead to new LEDs and display devices not only with precisely matched colors--better color accuracy and brightness--but also with improved performance lifetime and improved ease of manufacturing." QDs are already in use in display devices (e.g. Amazon Kindle and a new Samsung TV).

###

In addition to Lim and Smith, co-authors include Mohammad U. Zahid, Phuong Le, Liang Ma, Bioengineering at Illinois; David Entenberg, Allison S. Harney, and John Condeelis, Albert Einstein College of Medicine, New York.

Media Contact

Andrew M. Smith
smi@illinois.edu
217-300-5638

 @EngineeringAtIL

http://engineering.illinois.edu/ 

Andrew M. Smith | EurekAlert!

More articles from Physics and Astronomy:

nachricht Taking a spin on plasma space tornadoes with NASA observations
20.11.2017 | NASA/Goddard Space Flight Center

nachricht NASA detects solar flare pulses at Sun and Earth
17.11.2017 | NASA/Goddard Space Flight Center

All articles from Physics and Astronomy >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: A “cosmic snake” reveals the structure of remote galaxies

The formation of stars in distant galaxies is still largely unexplored. For the first time, astron-omers at the University of Geneva have now been able to closely observe a star system six billion light-years away. In doing so, they are confirming earlier simulations made by the University of Zurich. One special effect is made possible by the multiple reflections of images that run through the cosmos like a snake.

Today, astronomers have a pretty accurate idea of how stars were formed in the recent cosmic past. But do these laws also apply to older galaxies? For around a...

Im Focus: Visual intelligence is not the same as IQ

Just because someone is smart and well-motivated doesn't mean he or she can learn the visual skills needed to excel at tasks like matching fingerprints, interpreting medical X-rays, keeping track of aircraft on radar displays or forensic face matching.

That is the implication of a new study which shows for the first time that there is a broad range of differences in people's visual ability and that these...

Im Focus: Novel Nano-CT device creates high-resolution 3D-X-rays of tiny velvet worm legs

Computer Tomography (CT) is a standard procedure in hospitals, but so far, the technology has not been suitable for imaging extremely small objects. In PNAS, a team from the Technical University of Munich (TUM) describes a Nano-CT device that creates three-dimensional x-ray images at resolutions up to 100 nanometers. The first test application: Together with colleagues from the University of Kassel and Helmholtz-Zentrum Geesthacht the researchers analyzed the locomotory system of a velvet worm.

During a CT analysis, the object under investigation is x-rayed and a detector measures the respective amount of radiation absorbed from various angles....

Im Focus: Researchers Develop Data Bus for Quantum Computer

The quantum world is fragile; error correction codes are needed to protect the information stored in a quantum object from the deteriorating effects of noise. Quantum physicists in Innsbruck have developed a protocol to pass quantum information between differently encoded building blocks of a future quantum computer, such as processors and memories. Scientists may use this protocol in the future to build a data bus for quantum computers. The researchers have published their work in the journal Nature Communications.

Future quantum computers will be able to solve problems where conventional computers fail today. We are still far away from any large-scale implementation,...

Im Focus: Wrinkles give heat a jolt in pillared graphene

Rice University researchers test 3-D carbon nanostructures' thermal transport abilities

Pillared graphene would transfer heat better if the theoretical material had a few asymmetric junctions that caused wrinkles, according to Rice University...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Ecology Across Borders: International conference brings together 1,500 ecologists

15.11.2017 | Event News

Road into laboratory: Users discuss biaxial fatigue-testing for car and truck wheel

15.11.2017 | Event News

#Berlin5GWeek: The right network for Industry 4.0

30.10.2017 | Event News

 
Latest News

Antarctic landscape insights keep ice loss forecasts on the radar

20.11.2017 | Earth Sciences

Filling the gap: High-latitude volcanic eruptions also have global impact

20.11.2017 | Earth Sciences

Water world

20.11.2017 | Life Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>