While the new technology is years away from use in a clinical setting, the researchers are nonetheless confident that it will be useful in developing a microdevice that will help in understanding when prostate cancer will metastasize, or spread to other parts of the body.
Cancerous and non-cancerous cells are incubated with silver nanoparticle biotags, and then analyzed by shining the red laser on them. The biotags are shown on the cells' surface. Those glowing red in the middle are the cancer biomarkers, and those glowing green are standard biomarkers that bind to many cell types. A high ratio of red to green is found on the cancer cells. Credit: Gary Braun and Peter Allen/UCSB
"There have been studies to find the relationship between the number of cancer cells in the blood, and the outcome of the disease," said first author Alessia Pallaoro, postdoctoral fellow in UCSB's Department of Chemistry and Biochemistry. "The higher the number of cancer cells there are in the patient's blood, the worse the prognosis.
"The cancer cells that are found in the blood are thought to be the initiators of metastasis," Pallaoro added. "It would be really important to be able to find them and recognize them within blood or other bodily fluids. This could be helpful for diagnosis and follow-ups during treatment."
The researchers explained that although the primary tumor does not kill prostate cancer patients, metastasis does. "The delay is not well understood," said Gary Braun, second author and postdoctoral fellow in the Department of Molecular, Cellular, and Developmental Biology. "There is a big focus on understanding what causes the tumor to shed cells into the blood. If you could catch them all, then you could stop metastasis. The first thing is to monitor their appearance."
The team developed a novel technique to discriminate between cancerous and non-cancerous cells using a type of laser spectroscopy called surface enhanced Raman spectroscopy (SERS) and silver nanoparticles, which are biotags.
"Silver nanoparticles emit a rich set of colors when they absorb the laser light," said Braun. "This is different than fluorescence. This new technology could be more powerful than fluorescence."
The two types of biotags used in this research have a particular affinity that is dictated by the peptide they carry on their surface. One type attaches to a cell receptor called neuropilin-1, a recently described biomarker found on the surface membrane of certain cancer cells. The other biotag binds many cell types (both cancerous and non-cancerous) and serves as a standard measure as the cells are analyzed.
In this study, the team mixed the two biotags and added them to the healthy and tumor cell cultures. The average SERS signal over a given cell image yielded a ratio of the two signals consistent with the cells' known identity.
Pallaoro said she believes the most important part of the new technique is the fact that it could be expanded by adding more colors –– different particles of different colors –– as more biomarkers are found. The team used a new biomarker discovered by scientists at UCSB and the Sanford Burnham Medical Research Institute.
The senior author of the paper is Martin Moskovits, professor in UCSB's Department of Chemistry and Biochemistry.
Gail Gallessich | EurekAlert!
New vaccine production could improve flu shot accuracy
25.07.2017 | Duke University
Chances to treat childhood dementia
24.07.2017 | Julius-Maximilians-Universität Würzburg
Strong light-matter coupling in these semiconducting tubes may hold the key to electrically pumped lasers
Light-matter quasi-particles can be generated electrically in semiconducting carbon nanotubes. Material scientists and physicists from Heidelberg University...
Fraunhofer IPA has developed a proximity sensor made from silicone and carbon nanotubes (CNT) which detects objects and determines their position. The materials and printing process used mean that the sensor is extremely flexible, economical and can be used for large surfaces. Industry and research partners can use and further develop this innovation straight away.
At first glance, the proximity sensor appears to be nothing special: a thin, elastic layer of silicone onto which black square surfaces are printed, but these...
3-D shape acquisition using water displacement as the shape sensor for the reconstruction of complex objects
A global team of computer scientists and engineers have developed an innovative technique that more completely reconstructs challenging 3D objects. An ancient...
Physicists have developed a new technique that uses electrical voltages to control the electron spin on a chip. The newly-developed method provides protection from spin decay, meaning that the contained information can be maintained and transmitted over comparatively large distances, as has been demonstrated by a team from the University of Basel’s Department of Physics and the Swiss Nanoscience Institute. The results have been published in Physical Review X.
For several years, researchers have been trying to use the spin of an electron to store and transmit information. The spin of each electron is always coupled...
What is the mass of a proton? Scientists from Germany and Japan successfully did an important step towards the most exact knowledge of this fundamental constant. By means of precision measurements on a single proton, they could improve the precision by a factor of three and also correct the existing value.
To determine the mass of a single proton still more accurate – a group of physicists led by Klaus Blaum and Sven Sturm of the Max Planck Institute for Nuclear...
21.07.2017 | Event News
19.07.2017 | Event News
12.07.2017 | Event News
25.07.2017 | Physics and Astronomy
25.07.2017 | Earth Sciences
25.07.2017 | Life Sciences