The Leipzig Helmholtz Center for Environmental Research has gained new insights on the influence of tobacco smoke in utero. For the first time, it could be demonstrated with smoking pregnant women and their children, how exposure to tobacco smoke affects the development of human immune system on molecular level. The focus thereby was on microRNA - a short, single-stranded RNA molecule that is now recognised as playing an important role in gene regulation.
For some time now, the impact of environmental stressors during pregnancy on allergy risk among new-born children is a main research topic at Leipzig Helmholtz Center for Environmental Research (UFZ). As part of the long-term study LINA, environmental immunologists from Leipzig have been focussing on tobacco smoke as an environmental stressor. The main objective for Dr Gunda Herberth was to reveal the influence of tobacco smoke on the development of children's immune systems - at molecular level. From the results that were recently published in the "Journal of Allergy and Clinical Immunology", one thing is certain: "For the first time we were able to describe the effect of prenatal environmental stressors on the regulation of microRNA."
Former studies have already proven that smoking during pregnancy can harm the unborn child: Newborns from smoking mothers have shown low birth weights and impaired lung functions; later on in life respiratory diseases, diabetes type II, asthma or cardiovascular diseases were also more common. However, the exact molecular mechanisms and processes that are behind such developments still struggle researchers.
For this reason, Dr. Gunda Herberth and Dr. Irina Lehmann from the UFZ decided to address the relatively recent research area of microRNA. From the early 1990´s these cell components started to become more and more of a focus in molecular and cell biology. In the meantime, for humans more than 1,200 different short, single-stranded RNA molecules have been named, some of them playing an important role in immune response. Among others, they have a considerable influence on the differentiation of regulatory T cells (Treg cells), which in turn prevent an overactive immune system and thus autoimmune diseases. If there are insufficient Treg cells or if their function is impaired, the self-regulatory function of the immune system will be reduced, possibly resulting in allergies.
To investigate the relationship between smoking mothers during pregnancy on the one hand and their children´s risk of developing allergies on the other, the scientists from Leipzig examined microRNA-223, microRNA-155 and regulatory T cells - not only in the blood samples of pregnant women (36 weeks pregnant) but also at birth in the cord blood of their babies. At the same time, questionnaires were filled out and urine samples of the pregnant women were tested to substantiate the effect from exposure to tobacco smoke and/or from volatile organic compounds resulting from smoking. From the pool of mothers participating in the LINA-study, 315 mothers (6.6 percent of whom were smokers) and 441 children were consulted in these investigations.
The focus was on miR-223 and miR-155, because their role in regulating T cells had already been proven. "What we are now interested in finding out", explains Dr. Gunda Herberth , "is whether or not these microRNAs link exposure to smoke, regulatory T cells and the risk of developing allergies."
By measuring the concentration of these microRNAs as well as the number of regulatory T cells in maternal and cord blood it could be shown that a high exposure to inhaled volatile organic compounds (VOCs) associated with tobacco smoke coincides with high values for miR-223. At the same time it was also found that increased values for maternal and umbilical cord blood miR-223 correlate with low regulatory T-cell numbers. Finally, it could be shown that low regulatory T-cell numbers in umbilical cord blood was an indication that children exposed to tobacco smoke were more likely to develop an allergy before the age of three compared to those children with normal values for miR-223 and Treg cells. Furthermore, the probability of developing eczema was almost twice as high for these children."After already being able to demonstrate the influence of prenatal smoking on regulatory T-cell numbers in cord blood from our LINA study, the current epidemiological investigation delves even deeper into molecular processes", Dr. Gunda Herberth and Dr. Irina Lehmann resume. "Now", the immunologists from Leipzig explicate, "we will know more about the molecular processes that trigger off stressors from smoke during pregnancy." Thus, for the first time the association between prenatal environmental stressors and the regulation of microRNA is described. In this respect, the current Helmholtz study opens the door to further research on the role of microRNA in terms of how the human immune system reacts to environmental stressors. Daniela Weber
The Helmholtz Association contributes to finding solutions for large and pressing issues in society, science and the economy through excellence in the following six areas of research: energy, earth and the environment, health, key technologies, structure of matter, transport and aerospace. With almost 35,000 employees and coworkers in 18 research centres and an annual budget of approx. 3.8 billion Euros the Helmholtz Association is the largest scientific organization in Germany. Work is conducted in the tradition of the renowned natural scientist Hermann von Helmholtz (1821-1894). http://www.helmholtz.de/
Susanne Hufe | UFZ News
Further reports about: > Allergy > Environmental Research > RNA > RNA molecule > T cells > T-cell > UFZ > autoimmune disease > blood sample > cardiovascular disease > cord blood > environmental risk > environmental stress > environmental stressors > human immune system > immune system > mental stress > molecular process > pregnant women > respiratory disease > tobacco smoke > umbilical cord blood > vascular disease > volatile organic compounds > water resource
Researchers release the brakes on the immune system
18.10.2017 | Rheinische Friedrich-Wilhelms-Universität Bonn
Norovirus evades immune system by hiding out in rare gut cells
12.10.2017 | University of Pennsylvania School of Medicine
University of Maryland researchers contribute to historic detection of gravitational waves and light created by event
On August 17, 2017, at 12:41:04 UTC, scientists made the first direct observation of a merger between two neutron stars--the dense, collapsed cores that remain...
Seven new papers describe the first-ever detection of light from a gravitational wave source. The event, caused by two neutron stars colliding and merging together, was dubbed GW170817 because it sent ripples through space-time that reached Earth on 2017 August 17. Around the world, hundreds of excited astronomers mobilized quickly and were able to observe the event using numerous telescopes, providing a wealth of new data.
Previous detections of gravitational waves have all involved the merger of two black holes, a feat that won the 2017 Nobel Prize in Physics earlier this month....
Material defects in end products can quickly result in failures in many areas of industry, and have a massive impact on the safe use of their products. This is why, in the field of quality assurance, intelligent, nondestructive sensor systems play a key role. They allow testing components and parts in a rapid and cost-efficient manner without destroying the actual product or changing its surface. Experts from the Fraunhofer IZFP in Saarbrücken will be presenting two exhibits at the Blechexpo in Stuttgart from 7–10 November 2017 that allow fast, reliable, and automated characterization of materials and detection of defects (Hall 5, Booth 5306).
When quality testing uses time-consuming destructive test methods, it can result in enormous costs due to damaging or destroying the products. And given that...
Using a new cooling technique MPQ scientists succeed at observing collisions in a dense beam of cold and slow dipolar molecules.
How do chemical reactions proceed at extremely low temperatures? The answer requires the investigation of molecular samples that are cold, dense, and slow at...
Scientists from the Max Planck Institute of Quantum Optics, using high precision laser spectroscopy of atomic hydrogen, confirm the surprisingly small value of the proton radius determined from muonic hydrogen.
It was one of the breakthroughs of the year 2010: Laser spectroscopy of muonic hydrogen resulted in a value for the proton charge radius that was significantly...
17.10.2017 | Event News
10.10.2017 | Event News
10.10.2017 | Event News
20.10.2017 | Interdisciplinary Research
20.10.2017 | Materials Sciences
20.10.2017 | Earth Sciences