Consumption of undercooked cyst-laden meat from cattle, sheep and goats may cause infection in humans. Researchers from Universiti Teknologi MARA have successfully invented a PCR kit which provides a suitable and feasible means of screening, detection and identification with high sensitivity and specificity of the parasite.
Sarcosytis spp are intracellular protozoan parasites acquired upon consumption of undercooked cyst-laden meat from cattle, sheep and goats. Humans and some primates are defective hosts for Sarcosystis hominis and S. suihominis after ingesting raw meat from cattle and pigs, respectively.
Cases of human infection have been documented. Humans carry the intramuscular micro and macrocyst. The actual incidence among humans is yet to be assessed. The asexual stages, including sarcoyst, can provoke a strong inflammatory reaction in the tissues.
Human cases have been reported with acute myositis, diarrhoea and fever. Livestock suffer acute debilitating infections, resulting in abortion and death or chronic infections with failure to grow or thrive. It is indeed regrettable that this disease has not received the attention it deserves and remains a neglected one.
Studies have shown that sarcosytis spp were seen in 56% of hamburgers, 20% of hotdogs and sausages. Definitive diagnosis with identification of sporocysts in faeces requires multiple stool examinations, several days after having eaten the meat.
Sarcocystis sarcocysts in muscle biopsy specimens can be identified by microscopic examination of histological sections stained with haematoxylin and eosin and other stains such as PAS reaction. Basic histological examinations, by no means, can help definitive identification of sarcocystis at spies level and warrants electron microscopy or molecular analysis.
It has also been reported that polymerase chain reaction PCR positive samples were reported negative on histology. PCR provides a feasible means for screening, detection and identification with high sensitivity and specificity of the parasite.
Our PCR kit has been created with thermo stabilised PCR reactions Premixes consists of buffered salt solution with dNTPs, MgCl and Taq polymerase enzyme. The user just adds templates, primers and PCR grade distilled water and amplify based on the given conditions.
Advantages Amplifies multiple genes simultaneously requires no cold chain built-in gel loading dye which facilitates the loading of PCR products directly onto the agaose gel without addition of sample loading buffer, easy to follow steps minimises handling and pipetting useful for field works fast accurate and affordable PCR provides a suitable and feasible means for screening detection and identification with high sensitivity and specificity of the parasite.
Currently there are no commercially available PCR detection kits for sarcocystis. This kit can be of invaluable help for large scale quick screening of meat for sarcocystis. thus its value cannot be overemphasized for not only meat industry but also the ministry concerned with food and meat safety.
This innovation was awarded the Gold award in the Invention, Innovation and Design Exposition 2014 organized by Universiti Teknologi MARA.
For more information, contact
FACULTY OF MEDICINE
UNIVERSITI TEKNOLOGI MARA
New leukemia treatment offers hope
23.09.2016 | King Abdullah University of Science and Technology
Alzheimer’s: Cellular Mechanism Provides Explanation Model for Declining Memory Performance
21.09.2016 | Deutsches Zentrum für Neurodegenerative Erkrankungen e.V. (DZNE)
The Fraunhofer Institute for Organic Electronics, Electron Beam and Plasma Technology FEP has been developing various applications for OLED microdisplays based on organic semiconductors. By integrating the capabilities of an image sensor directly into the microdisplay, eye movements can be recorded by the smart glasses and utilized for guidance and control functions, as one example. The new design will be debuted at Augmented World Expo Europe (AWE) in Berlin at Booth B25, October 18th – 19th.
“Augmented-reality” and “wearables” have become terms we encounter almost daily. Both can make daily life a little simpler and provide valuable assistance for...
With the help of artificial intelligence, chemists from the University of Basel in Switzerland have computed the characteristics of about two million crystals made up of four chemical elements. The researchers were able to identify 90 previously unknown thermodynamically stable crystals that can be regarded as new materials. They report on their findings in the scientific journal Physical Review Letters.
Elpasolite is a glassy, transparent, shiny and soft mineral with a cubic crystal structure. First discovered in El Paso County (Colorado, USA), it can also be...
For the first time, Fraunhofer IKTS shows additively manufactured hardmetal tools at WorldPM 2016 in Hamburg. Mechanical, chemical as well as a high heat resistance and extreme hardness are required from tools that are used in mechanical and automotive engineering or in plastics and building materials industry. Researchers at the Fraunhofer Institute for Ceramic Technologies and Systems IKTS in Dresden managed the production of complex hardmetal tools via 3D printing in a quality that are in no way inferior to conventionally produced high-performance tools.
Fraunhofer IKTS counts decades of proven expertise in the development of hardmetals. To date, reliable cutting, drilling, pressing and stamping tools made of...
At AKL’16, the International Laser Technology Congress held in May this year, interest in the topic of process control was greater than expected. Appropriately, the event was also used to launch the Industry Working Group for Process Control in Laser Material Processing. The group provides a forum for representatives from industry and research to initiate pre-competitive projects and discuss issues such as standards, potential cost savings and feasibility.
In the age of industry 4.0, laser technology is firmly established within manufacturing. A wide variety of laser techniques – from USP ablation and additive...
Every three years, the plastics industry gathers at K, the international trade fair for plastics and rubber in Düsseldorf. The Fraunhofer Institute for Laser Technology ILT will also be attending again and presenting many innovative technologies, such as for joining plastics and metals using ultrashort pulse lasers. From October 19 to 26, you can find the Fraunhofer ILT at the joint Fraunhofer booth SC01 in Hall 7.
K is the world’s largest trade fair for the plastics and rubber industry. As in previous years, the organizers are expecting 3,000 exhibitors and more than...
23.09.2016 | Event News
20.09.2016 | Event News
16.09.2016 | Event News
23.09.2016 | Life Sciences
23.09.2016 | Health and Medicine
23.09.2016 | Life Sciences