Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Sarcocystis thermostable PCR detection kit

13.06.2014

Consumption of undercooked cyst-laden meat from cattle, sheep and goats may cause infection in humans. Researchers from Universiti Teknologi MARA have successfully invented a PCR kit which provides a suitable and feasible means of screening, detection and identification with high sensitivity and specificity of the parasite.

Sarcosytis spp are intracellular protozoan parasites acquired upon consumption of undercooked cyst-laden meat from cattle, sheep and goats. Humans and some primates are defective hosts for Sarcosystis hominis and S. suihominis after ingesting raw meat from cattle and pigs, respectively.

Cases of human infection have been documented. Humans carry the intramuscular micro and macrocyst. The actual incidence among humans is yet to be assessed. The asexual stages, including sarcoyst, can provoke a strong inflammatory reaction in the tissues.

Human cases have been reported with acute myositis, diarrhoea and fever. Livestock suffer acute debilitating infections, resulting in abortion and death or chronic infections with failure to grow or thrive. It is indeed regrettable that this disease has not received the attention it deserves and remains a neglected one.

Studies have shown that sarcosytis spp were seen in 56% of hamburgers, 20% of hotdogs and sausages. Definitive diagnosis with identification of sporocysts in faeces requires multiple stool examinations, several days after having eaten the meat.

Sarcocystis sarcocysts in muscle biopsy specimens can be identified by microscopic examination of histological sections stained with haematoxylin and eosin and other stains such as PAS reaction. Basic histological examinations, by no means, can help definitive identification of sarcocystis at spies level and warrants electron microscopy or molecular analysis.

It has also been reported that polymerase chain reaction PCR positive samples were reported negative on histology. PCR provides a feasible means for screening, detection and identification with high sensitivity and specificity of the parasite.

Our PCR kit has been created with thermo stabilised PCR reactions Premixes consists of buffered salt solution with dNTPs, MgCl and Taq polymerase enzyme. The user just adds templates, primers and PCR grade distilled water and amplify based on the given conditions.

Advantages Amplifies multiple genes simultaneously requires no cold chain built-in gel loading dye which facilitates the loading of PCR products directly onto the agaose gel without addition of sample loading buffer, easy to follow steps minimises handling and pipetting useful for field works fast accurate and affordable PCR provides a suitable and feasible means for screening detection and identification with high sensitivity and specificity of the parasite.

Currently there are no commercially available PCR detection kits for sarcocystis. This kit can be of invaluable help for large scale quick screening of meat for sarcocystis. thus its value cannot be overemphasized for not only meat industry but also the ministry concerned with food and meat safety.

This innovation was awarded the Gold award in the Invention, Innovation and Design Exposition 2014 organized by Universiti Teknologi MARA.

For more information, contact

Jamal Hussaini
FACULTY OF MEDICINE
UNIVERSITI TEKNOLOGI MARA
jamalh@salam.uitm.edu.my

Darmarajah Nadarajah | Research SEA News
Further information:
http://www.uitm.edu.my
http://www.researchsea.com

Further reports about: Teknologi UiTM conditions diarrhoea enzyme haematoxylin infections inflammatory meat parasite sarcosytis

More articles from Health and Medicine:

nachricht It don't mean a thing if the brain ain't got that swing
28.07.2015 | University of California - Berkeley

nachricht MSU scientists set sights on glaucoma medication to treat TB
24.07.2015 | Michigan State University

All articles from Health and Medicine >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Superfast fluorescence sets new speed record

Plasmonic device has speed and efficiency to serve optical computers

Researchers have developed an ultrafast light-emitting device that can flip on and off 90 billion times a second and could form the basis of optical computing.

Im Focus: Unlocking the rice immune system

Joint BioEnergy Institute study identifies bacterial protein that is key to protecting rice against bacterial blight

A bacterial signal that when recognized by rice plants enables the plants to resist a devastating blight disease has been identified by a multi-national team...

Im Focus: Smarter window materials can control light and energy

Researchers in the Cockrell School of Engineering at The University of Texas at Austin are one step closer to delivering smart windows with a new level of energy efficiency, engineering materials that allow windows to reveal light without transferring heat and, conversely, to block light while allowing heat transmission, as described in two new research papers.

By allowing indoor occupants to more precisely control the energy and sunlight passing through a window, the new materials could significantly reduce costs for...

Im Focus: Simulations lead to design of near-frictionless material

Argonne scientists used Mira to identify and improve a new mechanism for eliminating friction, which fed into the development of a hybrid material that exhibited superlubricity at the macroscale for the first time. Argonne Leadership Computing Facility (ALCF) researchers helped enable the groundbreaking simulations by overcoming a performance bottleneck that doubled the speed of the team's code.

While reviewing the simulation results of a promising new lubricant material, Argonne researcher Sanket Deshmukh stumbled upon a phenomenon that had never been...

Im Focus: NASA satellite camera provides 'EPIC' view of Earth

A NASA camera on the Deep Space Climate Observatory (DSCOVR) satellite has returned its first view of the entire sunlit side of Earth from one million miles away.

The color images of Earth from NASA's Earth Polychromatic Imaging Camera (EPIC) are generated by combining three separate images to create a...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

3rd Euro Bio-inspired - International Conference and Exhibition on Bio-inspired Materials

23.07.2015 | Event News

Clash of Realities – International Conference on the Art, Technology and Theory of Digital Games

10.07.2015 | Event News

World Conference on Regenerative Medicine in Leipzig: Last chance to submit abstracts until 2 July

25.06.2015 | Event News

 
Latest News

Possible Path Toward First Anti-MERS Drugs

28.07.2015 | Life Sciences

Smart Hydrogel Coating Creates “Stick-slip” Control of Capillary Action

28.07.2015 | Materials Sciences

Are Fish Getting High on Cocaine?

28.07.2015 | Ecology, The Environment and Conservation

VideoLinks
B2B-VideoLinks
More VideoLinks >>>