Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Sarcocystis thermostable PCR detection kit

13.06.2014

Consumption of undercooked cyst-laden meat from cattle, sheep and goats may cause infection in humans. Researchers from Universiti Teknologi MARA have successfully invented a PCR kit which provides a suitable and feasible means of screening, detection and identification with high sensitivity and specificity of the parasite.

Sarcosytis spp are intracellular protozoan parasites acquired upon consumption of undercooked cyst-laden meat from cattle, sheep and goats. Humans and some primates are defective hosts for Sarcosystis hominis and S. suihominis after ingesting raw meat from cattle and pigs, respectively.

Cases of human infection have been documented. Humans carry the intramuscular micro and macrocyst. The actual incidence among humans is yet to be assessed. The asexual stages, including sarcoyst, can provoke a strong inflammatory reaction in the tissues.

Human cases have been reported with acute myositis, diarrhoea and fever. Livestock suffer acute debilitating infections, resulting in abortion and death or chronic infections with failure to grow or thrive. It is indeed regrettable that this disease has not received the attention it deserves and remains a neglected one.

Studies have shown that sarcosytis spp were seen in 56% of hamburgers, 20% of hotdogs and sausages. Definitive diagnosis with identification of sporocysts in faeces requires multiple stool examinations, several days after having eaten the meat.

Sarcocystis sarcocysts in muscle biopsy specimens can be identified by microscopic examination of histological sections stained with haematoxylin and eosin and other stains such as PAS reaction. Basic histological examinations, by no means, can help definitive identification of sarcocystis at spies level and warrants electron microscopy or molecular analysis.

It has also been reported that polymerase chain reaction PCR positive samples were reported negative on histology. PCR provides a feasible means for screening, detection and identification with high sensitivity and specificity of the parasite.

Our PCR kit has been created with thermo stabilised PCR reactions Premixes consists of buffered salt solution with dNTPs, MgCl and Taq polymerase enzyme. The user just adds templates, primers and PCR grade distilled water and amplify based on the given conditions.

Advantages Amplifies multiple genes simultaneously requires no cold chain built-in gel loading dye which facilitates the loading of PCR products directly onto the agaose gel without addition of sample loading buffer, easy to follow steps minimises handling and pipetting useful for field works fast accurate and affordable PCR provides a suitable and feasible means for screening detection and identification with high sensitivity and specificity of the parasite.

Currently there are no commercially available PCR detection kits for sarcocystis. This kit can be of invaluable help for large scale quick screening of meat for sarcocystis. thus its value cannot be overemphasized for not only meat industry but also the ministry concerned with food and meat safety.

This innovation was awarded the Gold award in the Invention, Innovation and Design Exposition 2014 organized by Universiti Teknologi MARA.

For more information, contact

Jamal Hussaini
FACULTY OF MEDICINE
UNIVERSITI TEKNOLOGI MARA
jamalh@salam.uitm.edu.my

Darmarajah Nadarajah | Research SEA News
Further information:
http://www.uitm.edu.my
http://www.researchsea.com

Further reports about: Teknologi UiTM conditions diarrhoea enzyme haematoxylin infections inflammatory meat parasite sarcosytis

More articles from Health and Medicine:

nachricht Biofilm discovery suggests new way to prevent dangerous infections
23.05.2017 | University of Texas at Austin

nachricht Another reason to exercise: Burning bone fat -- a key to better bone health
19.05.2017 | University of North Carolina Health Care

All articles from Health and Medicine >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Can the immune system be boosted against Staphylococcus aureus by delivery of messenger RNA?

Staphylococcus aureus is a feared pathogen (MRSA, multi-resistant S. aureus) due to frequent resistances against many antibiotics, especially in hospital infections. Researchers at the Paul-Ehrlich-Institut have identified immunological processes that prevent a successful immune response directed against the pathogenic agent. The delivery of bacterial proteins with RNA adjuvant or messenger RNA (mRNA) into immune cells allows the re-direction of the immune response towards an active defense against S. aureus. This could be of significant importance for the development of an effective vaccine. PLOS Pathogens has published these research results online on 25 May 2017.

Staphylococcus aureus (S. aureus) is a bacterium that colonizes by far more than half of the skin and the mucosa of adults, usually without causing infections....

Im Focus: A quantum walk of photons

Physicists from the University of Würzburg are capable of generating identical looking single light particles at the push of a button. Two new studies now demonstrate the potential this method holds.

The quantum computer has fuelled the imagination of scientists for decades: It is based on fundamentally different phenomena than a conventional computer....

Im Focus: Turmoil in sluggish electrons’ existence

An international team of physicists has monitored the scattering behaviour of electrons in a non-conducting material in real-time. Their insights could be beneficial for radiotherapy.

We can refer to electrons in non-conducting materials as ‘sluggish’. Typically, they remain fixed in a location, deep inside an atomic composite. It is hence...

Im Focus: Wafer-thin Magnetic Materials Developed for Future Quantum Technologies

Two-dimensional magnetic structures are regarded as a promising material for new types of data storage, since the magnetic properties of individual molecular building blocks can be investigated and modified. For the first time, researchers have now produced a wafer-thin ferrimagnet, in which molecules with different magnetic centers arrange themselves on a gold surface to form a checkerboard pattern. Scientists at the Swiss Nanoscience Institute at the University of Basel and the Paul Scherrer Institute published their findings in the journal Nature Communications.

Ferrimagnets are composed of two centers which are magnetized at different strengths and point in opposing directions. Two-dimensional, quasi-flat ferrimagnets...

Im Focus: World's thinnest hologram paves path to new 3-D world

Nano-hologram paves way for integration of 3-D holography into everyday electronics

An Australian-Chinese research team has created the world's thinnest hologram, paving the way towards the integration of 3D holography into everyday...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Marine Conservation: IASS Contributes to UN Ocean Conference in New York on 5-9 June

24.05.2017 | Event News

AWK Aachen Machine Tool Colloquium 2017: Internet of Production for Agile Enterprises

23.05.2017 | Event News

Dortmund MST Conference presents Individualized Healthcare Solutions with micro and nanotechnology

22.05.2017 | Event News

 
Latest News

How herpesviruses win the footrace against the immune system

26.05.2017 | Life Sciences

Water forms 'spine of hydration' around DNA, group finds

26.05.2017 | Life Sciences

First Juno science results supported by University of Leicester's Jupiter 'forecast'

26.05.2017 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>