Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Sarcocystis thermostable PCR detection kit

13.06.2014

Consumption of undercooked cyst-laden meat from cattle, sheep and goats may cause infection in humans. Researchers from Universiti Teknologi MARA have successfully invented a PCR kit which provides a suitable and feasible means of screening, detection and identification with high sensitivity and specificity of the parasite.

Sarcosytis spp are intracellular protozoan parasites acquired upon consumption of undercooked cyst-laden meat from cattle, sheep and goats. Humans and some primates are defective hosts for Sarcosystis hominis and S. suihominis after ingesting raw meat from cattle and pigs, respectively.

Cases of human infection have been documented. Humans carry the intramuscular micro and macrocyst. The actual incidence among humans is yet to be assessed. The asexual stages, including sarcoyst, can provoke a strong inflammatory reaction in the tissues.

Human cases have been reported with acute myositis, diarrhoea and fever. Livestock suffer acute debilitating infections, resulting in abortion and death or chronic infections with failure to grow or thrive. It is indeed regrettable that this disease has not received the attention it deserves and remains a neglected one.

Studies have shown that sarcosytis spp were seen in 56% of hamburgers, 20% of hotdogs and sausages. Definitive diagnosis with identification of sporocysts in faeces requires multiple stool examinations, several days after having eaten the meat.

Sarcocystis sarcocysts in muscle biopsy specimens can be identified by microscopic examination of histological sections stained with haematoxylin and eosin and other stains such as PAS reaction. Basic histological examinations, by no means, can help definitive identification of sarcocystis at spies level and warrants electron microscopy or molecular analysis.

It has also been reported that polymerase chain reaction PCR positive samples were reported negative on histology. PCR provides a feasible means for screening, detection and identification with high sensitivity and specificity of the parasite.

Our PCR kit has been created with thermo stabilised PCR reactions Premixes consists of buffered salt solution with dNTPs, MgCl and Taq polymerase enzyme. The user just adds templates, primers and PCR grade distilled water and amplify based on the given conditions.

Advantages Amplifies multiple genes simultaneously requires no cold chain built-in gel loading dye which facilitates the loading of PCR products directly onto the agaose gel without addition of sample loading buffer, easy to follow steps minimises handling and pipetting useful for field works fast accurate and affordable PCR provides a suitable and feasible means for screening detection and identification with high sensitivity and specificity of the parasite.

Currently there are no commercially available PCR detection kits for sarcocystis. This kit can be of invaluable help for large scale quick screening of meat for sarcocystis. thus its value cannot be overemphasized for not only meat industry but also the ministry concerned with food and meat safety.

This innovation was awarded the Gold award in the Invention, Innovation and Design Exposition 2014 organized by Universiti Teknologi MARA.

For more information, contact

Jamal Hussaini
FACULTY OF MEDICINE
UNIVERSITI TEKNOLOGI MARA
jamalh@salam.uitm.edu.my

Darmarajah Nadarajah | Research SEA News
Further information:
http://www.uitm.edu.my
http://www.researchsea.com

Further reports about: Teknologi UiTM conditions diarrhoea enzyme haematoxylin infections inflammatory meat parasite sarcosytis

More articles from Health and Medicine:

nachricht Penn studies find promise for innovations in liquid biopsies
30.03.2017 | University of Pennsylvania School of Medicine

nachricht 'On-off switch' brings researchers a step closer to potential HIV vaccine
30.03.2017 | University of Nebraska-Lincoln

All articles from Health and Medicine >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: A Challenging European Research Project to Develop New Tiny Microscopes

The Institute of Semiconductor Technology and the Institute of Physical and Theoretical Chemistry, both members of the Laboratory for Emerging Nanometrology (LENA), at Technische Universität Braunschweig are partners in a new European research project entitled ChipScope, which aims to develop a completely new and extremely small optical microscope capable of observing the interior of living cells in real time. A consortium of 7 partners from 5 countries will tackle this issue with very ambitious objectives during a four-year research program.

To demonstrate the usefulness of this new scientific tool, at the end of the project the developed chip-sized microscope will be used to observe in real-time...

Im Focus: Giant Magnetic Fields in the Universe

Astronomers from Bonn and Tautenburg in Thuringia (Germany) used the 100-m radio telescope at Effelsberg to observe several galaxy clusters. At the edges of these large accumulations of dark matter, stellar systems (galaxies), hot gas, and charged particles, they found magnetic fields that are exceptionally ordered over distances of many million light years. This makes them the most extended magnetic fields in the universe known so far.

The results will be published on March 22 in the journal „Astronomy & Astrophysics“.

Galaxy clusters are the largest gravitationally bound structures in the universe. With a typical extent of about 10 million light years, i.e. 100 times the...

Im Focus: Tracing down linear ubiquitination

Researchers at the Goethe University Frankfurt, together with partners from the University of Tübingen in Germany and Queen Mary University as well as Francis Crick Institute from London (UK) have developed a novel technology to decipher the secret ubiquitin code.

Ubiquitin is a small protein that can be linked to other cellular proteins, thereby controlling and modulating their functions. The attachment occurs in many...

Im Focus: Perovskite edges can be tuned for optoelectronic performance

Layered 2D material improves efficiency for solar cells and LEDs

In the eternal search for next generation high-efficiency solar cells and LEDs, scientists at Los Alamos National Laboratory and their partners are creating...

Im Focus: Polymer-coated silicon nanosheets as alternative to graphene: A perfect team for nanoelectronics

Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are less stable. Now researchers at the Technical University of Munich (TUM) have, for the first time ever, produced a composite material combining silicon nanosheets and a polymer that is both UV-resistant and easy to process. This brings the scientists a significant step closer to industrial applications like flexible displays and photosensors.

Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

International Land Use Symposium ILUS 2017: Call for Abstracts and Registration open

20.03.2017 | Event News

CONNECT 2017: International congress on connective tissue

14.03.2017 | Event News

ICTM Conference: Turbine Construction between Big Data and Additive Manufacturing

07.03.2017 | Event News

 
Latest News

'On-off switch' brings researchers a step closer to potential HIV vaccine

30.03.2017 | Health and Medicine

Penn studies find promise for innovations in liquid biopsies

30.03.2017 | Health and Medicine

An LED-based device for imaging radiation induced skin damage

30.03.2017 | Medical Engineering

VideoLinks
B2B-VideoLinks
More VideoLinks >>>