Instead of red, white or black plastic, however, the wiring in the nervous system is protected by layers of an insulating protein called myelin. These layers increase the speed that nerve impulses travel throughout the brain and the body. The critical role they play is dramatically illustrated by the symptoms of multiple sclerosis, which is caused by lesions that destroy myelin. These include blindness, muscle weakness and paralysis, loss of coordination, stuttering, pain and burning sensations, impotence, memory loss, depression and dementia.
The formation of myelin sheaths during development requires a complex choreography generally considered to be one of nature’s most spectacular examples of the interaction between different kinds of cells. Now, a group of Vanderbilt researchers has successfully produced movies that provide the first direct view of the initial stage of this process. That’s the period when the cells that ultimately produce the myelin sheathing spread throughout the developing nervous system. The results were published online in the journal Nature Neuroscience on Nov. 12 and should aid in the design of new therapies to promote the repair of this protective layer following disease or injury.
“We discovered that this process is far more dynamic than anyone had dreamed,” says Bruce Appel, the associate professor of biological sciences and Kennedy Center investigator who headed up the study.
In the central nervous system, the myelin membranes are produced by cells called oligodendrocytes. These cells must be distributed uniformly along axons – the long, wire-like extensions from neurons that carry nerve impulses – so that the membranes, which wrap the nerve fibers like millions of microscopic pieces of electrician’s tape, can cover the axons completely and uniformly. The wrapping process takes place near the end of fetal development and actually continues for some time after birth.
In order to study this process, Appel and his research group – graduate students Brandon Kirby and Jimann Shin along with post doctoral fellows Norio Takada and Andrew Latimer – created a transgenic zebrafish which incorporates fluorescent proteins in the cells involved in myelination. The zebrafish is a small tropical fish that has become a popular species for studying the process of development in vertebrates (animals with backbones). Because zebrafish embryos are transparent and develop within a few days, they allow biologists to watch developmental processes as they take place: something they cannot do with mice or other mammals. These characteristics allowed the Vanderbilt researchers to obtain images of the cells involved in myelination using a confocal microscope and edit them into time-lapse movies.
The oligodendrocytes that produce the myelin membranes arise from mobile, dividing cells called “oligodentrocyte progenitor cells” or OPCs. OPCs are made in special locations in the brain and spinal cord. These cells seek out axons and spread out along them. Then, at a certain point, a fraction of the OPCs transform themselves into oligodentrocytes and begin wrapping axons. Each cell can wrap portions of several different axons and each axon is wrapped by a large number of oligodentrocytes.
Before the Vanderbilt study, there were a number of different theories about how OPCs space themselves along axons. One was that the axons themselves produce some kind of positional cues that the OPCs follow. Another was that the OPCs sense each other and adjust their position accordingly: a mechanism somewhat similar to that which soldiers on the parade ground use to align a formation by extending their right arm and adjusting their position until their outstretched fingers touch the shoulder of the person on the right.
Previous studies of OPCs grown in tissue culture had seen that they could generate small pseudopods, called filopodia, but no one knew what their purpose might be. So, when the researchers began viewing their movies, they were excited to observe that the cells were continually sending out filopodia in different directions. They found that OPCs not only generate these tiny tentacles, but keep them extending and contracting in a fashion reminiscent of the party noise-makers called blow-outs that unroll when you blow on them and snap back when you stop. They observed that when one of these tiny tentacles touches a neighboring OPC, the cells react by moving in the opposite direction. This caused a surprising amount of movement as the OPCs repeatedly readjusted their positions.
“This could serve as a surveillance mechanism that allows the OPCs to determine the presence or absence of nearby cells of the same type,” says Appel, “and could explain how they distribute themselves along the axons.”
The researchers used the same system to see how the OPCs respond to injuries and conditions like multiple sclerosis. They did so by using a laser to destroy the OPCs along a short length of the embryo’s spine a day before the axon-wrapping stage begins. They found that the OPCs in the vicinity of the gap start dividing to produce additional cells that move into the gap. After a day, the number of OPCs in the gap had grown to 50 percent of normal and after four days it had risen to 70 percent.
“Now that we have a better understanding of OPC and oligodendrocyte behaviors, we are in a much better position to identify and study the genes that are necessary for myelination,” says Appel, “and having these genes in hand should aid in the design of drugs to promote remyelination following disease or injury.”
David F. Salisbury | Vanderbilt University
Another reason to exercise: Burning bone fat -- a key to better bone health
19.05.2017 | University of North Carolina Health Care
Disrupted fat breakdown in the brain makes mice dumb
19.05.2017 | Rheinische Friedrich-Wilhelms-Universität Bonn
Two-dimensional magnetic structures are regarded as a promising material for new types of data storage, since the magnetic properties of individual molecular building blocks can be investigated and modified. For the first time, researchers have now produced a wafer-thin ferrimagnet, in which molecules with different magnetic centers arrange themselves on a gold surface to form a checkerboard pattern. Scientists at the Swiss Nanoscience Institute at the University of Basel and the Paul Scherrer Institute published their findings in the journal Nature Communications.
Ferrimagnets are composed of two centers which are magnetized at different strengths and point in opposing directions. Two-dimensional, quasi-flat ferrimagnets...
An Australian-Chinese research team has created the world's thinnest hologram, paving the way towards the integration of 3D holography into everyday...
In the race to produce a quantum computer, a number of projects are seeking a way to create quantum bits -- or qubits -- that are stable, meaning they are not much affected by changes in their environment. This normally needs highly nonlinear non-dissipative elements capable of functioning at very low temperatures.
In pursuit of this goal, researchers at EPFL's Laboratory of Photonics and Quantum Measurements LPQM (STI/SB), have investigated a nonlinear graphene-based...
Dental plaque and the viscous brown slime in drainpipes are two familiar examples of bacterial biofilms. Removing such bacterial depositions from surfaces is...
For the first time, scientists have succeeded in studying the strength of hydrogen bonds in a single molecule using an atomic force microscope. Researchers from the University of Basel’s Swiss Nanoscience Institute network have reported the results in the journal Science Advances.
Hydrogen is the most common element in the universe and is an integral part of almost all organic compounds. Molecules and sections of macromolecules are...
22.05.2017 | Event News
17.05.2017 | Event News
16.05.2017 | Event News
22.05.2017 | Materials Sciences
22.05.2017 | Life Sciences
22.05.2017 | Physics and Astronomy