Thanks to a new imaging system, researchers at MIT's Picower Institute for Learning and Memory have gotten an unprecedented look into how genes shape the brain in response to the environment. Their work will be reported in the July 28 issue of Cell.
"This work represents a technological breakthrough," said first author Kuan Hong Wang, a research scientist at the Picower Institute who will launch his own laboratory at the National Institute of Mental Health in the fall. "This is the first study that demonstrates the ability to directly visualize the molecular activity of individual neurons in the brain of live animals at a single-cell resolution, and to observe the changes in the activity in the same neurons in response to the changes of the environment on a daily basis for a week."
This advance, coupled with other brain disease models, could "offer unparalleled advantages in understanding pathological processes in real time, leading to potential new drugs and treatments for a host of neurological diseases and mental disorders," said Nobel laureate Susumu Tonegawa, a co-author of the study.
Tonegawa, director of the Picower Institute and the Picower Professor of Biology and Neuroscience at MIT, Wang and colleagues found that visual experience induces a protein that works as a molecular "filter" to enhance the overall selectivity of the brain's responses to visual stimuli.
The protein, called "Arc," was previously detected in the hippocampus, where it is believed to help store lasting memories by strengthening synapses, the connections between neurons. The Picower Institute's unexpected finding was that Arc also blocks the activity of neurons with low orientation selectivity that are not well "tuned" to vertical and horizontal lines, while keeping neurons with high orientation selectivity.
"Consequently, with the help of Arc, the overall orientation selectivity in the visual cortex is sharpened by visual experience," like a camera that learns to focus better over time, Wang said. What's more, he said, "we suspect that this molecular filtering mechanism may also be applicable to other information processing systems in the brain."
Although baby animals are born with a handful of neurons tuned to respond to edges of light at specific orientations, the ability to detect these orientations improves with experience. The more the animal is exposed to shapes, objects and light, the better it can perceive them.
Plasticity is the amazing ability of a neuron or a synapse to change in response to experience. Changes in synaptic strength require rapid protein synthesis, but at the molecular level, little is known about the factors contributing to experience-dependent changes in orientation selectivity in the visual cortex, Wang said.
To come up with a better way to investigate this, the MIT team developed a state-of-the-art imaging system in which transparent cranial windows were implanted over the primary visual cortex, allowing the researchers to monitor over time the expression of proteins in the brains of live mice.
The study exploited the power of two-photon microscopy (so-called because it uses two infrared photons to emit fluorescence in tissue), which allows imaging of living tissue up to 1 millimeter deep, enough for researchers to see proteins expressed within individual neurons within the brain.
They then created a mouse model in which a coding portion of the Arc gene was replaced with a jellyfish gene encoding a green fluorescent protein (GFP). Neural activities that normally activate the Arc gene then activated the GFP, leaving a fluorescent trace detectable by two-photon microscopy. This allowed the researchers to image neuronal activation patterns induced by visual experience, thus uncovering the Arc protein's role in orchestrating neurons' reactions to natural sensory stimuli.
The genetically engineered mice were let loose in an environment containing a cylinder covered with stripes of vertical or horizontal lines, and the proteins in their brains were monitored as the mice saw the cylinders daily.
In addition to Tonegawa and Wang, authors include former Picower Institute postdoctoral fellow Ania Majewska; Picower Institute postdoctoral associate James Schummers; Brandon J. Farley, a graduate student in MIT's Department of Brain and Cognitive Sciences; Mriganka Sur, head of the Department of Brain and Cognitive Sciences and Sherman Fairchild Professor of Neuroscience; and Chengcheng Hu, a professor at Harvard's School of Public Health.
This work is supported by the National Institutes of Health, the Howard Hughes Medical Institute and the RIKEN-MIT Neuroscience Research Center.
Elizabeth A. Thomson | MIT News Office
Study suggests possible new target for treating and preventing Alzheimer's
02.12.2016 | Oregon Health & Science University
The first analysis of Ewing's sarcoma methyloma opens doors to new treatments
01.12.2016 | IDIBELL-Bellvitge Biomedical Research Institute
A multi-institutional research collaboration has created a novel approach for fabricating three-dimensional micro-optics through the shape-defined formation of porous silicon (PSi), with broad impacts in integrated optoelectronics, imaging, and photovoltaics.
Working with colleagues at Stanford and The Dow Chemical Company, researchers at the University of Illinois at Urbana-Champaign fabricated 3-D birefringent...
In experiments with magnetic atoms conducted at extremely low temperatures, scientists have demonstrated a unique phase of matter: The atoms form a new type of quantum liquid or quantum droplet state. These so called quantum droplets may preserve their form in absence of external confinement because of quantum effects. The joint team of experimental physicists from Innsbruck and theoretical physicists from Hannover report on their findings in the journal Physical Review X.
“Our Quantum droplets are in the gas phase but they still drop like a rock,” explains experimental physicist Francesca Ferlaino when talking about the...
The Max Planck Institute for Physics (MPP) is opening up a new research field. A workshop from November 21 - 22, 2016 will mark the start of activities for an innovative axion experiment. Axions are still only purely hypothetical particles. Their detection could solve two fundamental problems in particle physics: What dark matter consists of and why it has not yet been possible to directly observe a CP violation for the strong interaction.
The “MADMAX” project is the MPP’s commitment to axion research. Axions are so far only a theoretical prediction and are difficult to detect: on the one hand,...
Broadband rotational spectroscopy unravels structural reshaping of isolated molecules in the gas phase to accommodate water
In two recent publications in the Journal of Chemical Physics and in the Journal of Physical Chemistry Letters, researchers around Melanie Schnell from the Max...
The efficiency of power electronic systems is not solely dependent on electrical efficiency but also on weight, for example, in mobile systems. When the weight of relevant components and devices in airplanes, for instance, is reduced, fuel savings can be achieved and correspondingly greenhouse gas emissions decreased. New materials and components based on gallium nitride (GaN) can help to reduce weight and increase the efficiency. With these new materials, power electronic switches can be operated at higher switching frequency, resulting in higher power density and lower material costs.
Researchers at the Fraunhofer Institute for Solar Energy Systems ISE together with partners have investigated how these materials can be used to make power...
16.11.2016 | Event News
01.11.2016 | Event News
14.10.2016 | Event News
02.12.2016 | Medical Engineering
02.12.2016 | Agricultural and Forestry Science
02.12.2016 | Physics and Astronomy