Specialists of the Gamalei Research Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences, and the Botkin Clinical Hospital have developed the method that allows to significantly simplify examination of patients suffering from stomach and duodenal diseases caused by the Helicobacter pylori bacteria. The researchers have created immunological test-system capable to discover the pathogene toxin in the patients’ saliva and faeces without resorting to traditional complicated and expensive diagnostics methods.
Stomach and duodenal ulcers are often of bacterial nature. The ulser pathogene, the Helicobacter pylori bacterium, is dangerous by its cytotoxin, in the presence of which the ulcer more often develops into a malignant form. The contemporary medicine possesses a lot of sensitive methods for diagnostics of bacteria and their proteins, but, unfortunately, many of them are complicated and expensive. The majority of methods require penetration into the stomach or the duodenum, thus they are not always suitable for examination of children and bad cases. The necessity for a simplier examination method is obviously imminent.
Physicians suggest to use diagnostic antiserum to VacA toxin protein for simplified diagnostics of Helicobacteriosis. The researchers have cloned a small tail fragment of this protein’s gene. The gene worked duly in the E. coli and produced the protein in the quantities sufficient for immunization. Rabbits were immunized by the purified protein, and serum specific for Helicobacter cytotoxin was produced from their blood. The test-system was tried on real clinical material – saliva and faeces samples of chronic invalids during various exacerbation periods, and before the deiseas and after the conducted treatment.
Sergey Komarov | alfa
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