Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Georgetown cancer researchers develop high throughput method

27.06.2005


Cutting edge matrix assembly can be used to analyze large numbers of tumors



Scientists at Georgetown University’s Lombardi Comprehensive Cancer Center have devised a new low-cost technology that allows thousands of tumor slices to be screened side-by-side, an improvement over current and more expensive methods that can analyze only several hundred tumors at once. The researchers anticipate that this technology could someday lead to more reliable prediction of patient prognosis and improved selection of optimal treatments for cancer and other diseases.

The new technology, details of which are published in the July 2005 issue of Nature Methods, "may lead to a better understanding of human cancer, as well as other human disorders, because it will let scientists discover and then detect unique biomarkers of disease in patients," says Hallgeir Rui, M.D., Ph.D., associate professor of oncology at Georgetown and principal investigator of the study.


Rui and the study’s first author, postdoctoral researcher Matthew LeBaron, Ph.D., created the technology, which they call cutting edge matrix assembly (CEMA), to construct what are known in the field as tissue microarrays. This new method can be done by using the tools that are already available in a medical center’s pathology laboratory, they say.

Researchers now analyze tumors or tissues in large numbers by embedding cylindrical core samples of tissue, each taken from an individual patient, into a cube-like paraffin block, which is then sliced thin and stained in order to show proteins or molecules that scientists think may be involved in a disease. The cores, however, must be spaced a certain distance apart within the paraffin structure or else the cube will crack, Rui says. "This is both laborious and tricky."

The CEMA technology uses a simple strategy of stacking "plates" of individual tissue, and bonding them with glue. The multiple stacks are then transversely cut and bonded edge-to-edge to assemble the high density arrays or matrices. These arrays, which are then also thinly sliced for analysis, can hold more than 10,000 tissue samples, the researchers say.

"Just like cars used to be built on a heavy frame but are now assembled with a self-supporting construction, CEMA arrays do not require a space-wasting scaffold or frame but samples are instead bonded directly to each other," Rui says.

"The statistical power inherent in the larger sample numbers of CEMA arrays are expected to strengthen the discovery of new diagnostic markers," LeBaron says. "Such markers will allow more accurate patient diagnosis and predict outcome more effectively, and ultimately tailor treatments to an individual’s disease."

"In addition to tumor analyses, CEMA arrays will be useful for large scale studies of whether drugs or environmental contaminants have toxic effects on healthy tissues," says Heidi Crismon, a medical student who assembled the so far largest array of nearly 12,000 individual pieces of liver and kidney tissues for this study.

With CEMA, the investigators have also solved the problem of creating arrays of thin-walled or multilayered tissues such as intestine, skin, and blood vessels which can not be arrayed by existing technologies.

Cindy Fox Aisen | EurekAlert!
Further information:
http://www.georgetown.edu

More articles from Health and Medicine:

nachricht Laser activated gold pyramids could deliver drugs, DNA into cells without harm
24.03.2017 | Harvard John A. Paulson School of Engineering and Applied Sciences

nachricht What does congenital Zika syndrome look like?
24.03.2017 | University of California - San Diego

All articles from Health and Medicine >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Giant Magnetic Fields in the Universe

Astronomers from Bonn and Tautenburg in Thuringia (Germany) used the 100-m radio telescope at Effelsberg to observe several galaxy clusters. At the edges of these large accumulations of dark matter, stellar systems (galaxies), hot gas, and charged particles, they found magnetic fields that are exceptionally ordered over distances of many million light years. This makes them the most extended magnetic fields in the universe known so far.

The results will be published on March 22 in the journal „Astronomy & Astrophysics“.

Galaxy clusters are the largest gravitationally bound structures in the universe. With a typical extent of about 10 million light years, i.e. 100 times the...

Im Focus: Tracing down linear ubiquitination

Researchers at the Goethe University Frankfurt, together with partners from the University of Tübingen in Germany and Queen Mary University as well as Francis Crick Institute from London (UK) have developed a novel technology to decipher the secret ubiquitin code.

Ubiquitin is a small protein that can be linked to other cellular proteins, thereby controlling and modulating their functions. The attachment occurs in many...

Im Focus: Perovskite edges can be tuned for optoelectronic performance

Layered 2D material improves efficiency for solar cells and LEDs

In the eternal search for next generation high-efficiency solar cells and LEDs, scientists at Los Alamos National Laboratory and their partners are creating...

Im Focus: Polymer-coated silicon nanosheets as alternative to graphene: A perfect team for nanoelectronics

Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are less stable. Now researchers at the Technical University of Munich (TUM) have, for the first time ever, produced a composite material combining silicon nanosheets and a polymer that is both UV-resistant and easy to process. This brings the scientists a significant step closer to industrial applications like flexible displays and photosensors.

Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are...

Im Focus: Researchers Imitate Molecular Crowding in Cells

Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to simulate these confined natural conditions in artificial vesicles for the first time. As reported in the academic journal Small, the results are offering better insight into the development of nanoreactors and artificial organelles.

Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

International Land Use Symposium ILUS 2017: Call for Abstracts and Registration open

20.03.2017 | Event News

CONNECT 2017: International congress on connective tissue

14.03.2017 | Event News

ICTM Conference: Turbine Construction between Big Data and Additive Manufacturing

07.03.2017 | Event News

 
Latest News

Argon is not the 'dope' for metallic hydrogen

24.03.2017 | Materials Sciences

Astronomers find unexpected, dust-obscured star formation in distant galaxy

24.03.2017 | Physics and Astronomy

Gravitational wave kicks monster black hole out of galactic core

24.03.2017 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>