ESC Congress 2004: Embryonic endothelial progenitor cells help overcome myocardial infarction in pigs
Myocardial infarction, caused by coronary artery occlusion, can lead up to loss of muscle tissue of the heart and functional detriment, even at times where rapid reperfusion strategies like PTCA or thrombolysis are at hand. In the study presented here, we investigated embryonic endothelial progenitor cells as therapy for ischemia reperfusion injury in a large animal model (pig). This model, which induces an infarct of predictable size in a pig heart, was used to test the cardioprotective potential of the embryonic cells, since adult endothelial progenitor cells (EPCs) have been used in similar models with success and are utilized in ongoing patient studies.
Endothelial progenitor cells are cells which are able to differentiate into endothelial cells and replace the inner vascular wall.In this study, we used 5x106 embryonic endothelial progenitor cells, a number relatively modest with respect to the size of the targeted infarct region. To compensate for this modest cell number, we used a regional delivery system, called retroinfusion, which infuses the cells through the vein draining the infarct region. (Previous studies had shown a substantial increase of efficacy using this application mode).
We now found that indeed retroinfusion of 5x106 embryonic EPCs sufficed to reduce infarct size and improve regional myocardial function in the ischemic area. Interestingly, systemic application of the same number of cells had no significant effect, indicating the relevance of the regional application. The superiority of the regional delivery was confirmed in tests using radioactively labeled cells, where retroinfusion yielded a sixfold higher amount of recruited cells in the heart than systemic application.
Currently, embryonic EPCs are an experimental tool trying to investigate the pathways utilized by these cells to protect the ischemic heart. A variety of efforts is underway to characterize the embryonic EPCs further and to potentially enhance their performance. Because of species differences, it is unclear whether a similar approach can be used in patients.
However, a similar cell line might be derived from human embryonic stem cells, and become a helpful tool for ischemia/reperfusion injury of the heart in the future. C Kupatt (Munich, DE)
Camilla Dormer | alfa
The most recent press releases about innovation >>>
Die letzten 5 Focus-News des innovations-reports im Überblick:
Researchers from the University of Hamburg in Germany, in collaboration with colleagues from the University of Aarhus in Denmark, have synthesized a new superconducting material by growing a few layers of an antiferromagnetic transition-metal chalcogenide on a bismuth-based topological insulator, both being non-superconducting materials.
While superconductivity and magnetism are generally believed to be mutually exclusive, surprisingly, in this new material, superconducting correlations...
Laser-driving of semimetals allows creating novel quasiparticle states within condensed matter systems and switching between different states on ultrafast time scales
Studying properties of fundamental particles in condensed matter systems is a promising approach to quantum field theory. Quasiparticles offer the opportunity...
Among the general public, solar thermal energy is currently associated with dark blue, rectangular collectors on building roofs. Technologies are needed for aesthetically high quality architecture which offer the architect more room for manoeuvre when it comes to low- and plus-energy buildings. With the “ArKol” project, researchers at Fraunhofer ISE together with partners are currently developing two façade collectors for solar thermal energy generation, which permit a high degree of design flexibility: a strip collector for opaque façade sections and a solar thermal blind for transparent sections. The current state of the two developments will be presented at the BAU 2017 trade fair.
As part of the “ArKol – development of architecturally highly integrated façade collectors with heat pipes” project, Fraunhofer ISE together with its partners...
At TU Wien, an alternative for resource intensive formwork for the construction of concrete domes was developed. It is now used in a test dome for the Austrian Federal Railways Infrastructure (ÖBB Infrastruktur).
Concrete shells are efficient structures, but not very resource efficient. The formwork for the construction of concrete domes alone requires a high amount of...
Many pathogens use certain sugar compounds from their host to help conceal themselves against the immune system. Scientists at the University of Bonn have now, in cooperation with researchers at the University of York in the United Kingdom, analyzed the dynamics of a bacterial molecule that is involved in this process. They demonstrate that the protein grabs onto the sugar molecule with a Pac Man-like chewing motion and holds it until it can be used. Their results could help design therapeutics that could make the protein poorer at grabbing and holding and hence compromise the pathogen in the host. The study has now been published in “Biophysical Journal”.
The cells of the mouth, nose and intestinal mucosa produce large quantities of a chemical called sialic acid. Many bacteria possess a special transport system...