Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

New technology blocks gene to increase immune response against deadly brain tumor cells

26.05.2004


With new technology that uses short strands of genetic material to shut down a specific gene, researchers have regulated immune system proteins to boost production of cells that seek and destroy cancer cells. This approach may improve the effectiveness of vaccines in the treatment of tumors, including malignant brain tumors.



Results of the study appear in the June issue of the European Journal of Immunology, and the research was conducted at Cedars-Sinai’s Maxine Dunitz Neurosurgical Institute, where clinical trials of dendritic cell immunotherapy have been underway for several years.

Dendritic cells are the immune system’s most potent antigen-presenting cells – those that identify "foreign" substances for destruction. Because cancer cells often are not recognized by dendritic cells as antigens, the neurosurgeons and other scientists at the Institute have developed and studied a vaccine in the treatment of highly aggressive brain tumors called gliomas. They combine in the laboratory tumor cells that have been surgically removed and dendritic cells derived from a patient’s blood. The new cells are injected back into the patient to seek out other cancer cells for destruction.


Early clinical trials have shown an increase in survival rates among patients receiving the dendritic cell vaccine. Meanwhile, Institute researchers have been studying underlying genetic and cellular mechanisms as well as other methods for increasing immune response and enhancing the vaccine’s effectiveness.

"This study demonstrates that by turning off the interleukin 10 gene in the dendritic cell we can make a much more effective dendritic cell in terms of generating a significant immune response," said John S. Yu, MD, the article’s senior author and co-director of the Comprehensive Brain Tumor Program at Cedars-Sinai.

One of the functions of dendritic cells is to influence immature T cells to become either T helper type 1 (Th1) or T helper type 2 (Th2) cells. A naturally occurring protein, interleukin 12 (IL-12), interacting with dendritic cells, spurs the development of Th1 cells. Interleukin 10 (IL-10) inhibits the production of IL-12.

"Interleukin 10 is a molecule that generates a Th2 response, which is effective against organisms such as bacteria, but for a tumor treatment a Th1 response is our goal. The Th1 response is generated through T cells against a tumor," said Dr. Yu.

In this study, the researchers used a new approach called RNA interference to target the IL-10 gene, inserting short strands of synthetic IL-10 specific RNA (small interfering RNA or siRNA) into dendritic cells generated from peripheral blood cells. Suppression of the IL-10 gene inhibited the secretion of the IL-10 protein, which allowed increased production of IL-12. Naïve T cells co-cultured with siRNA-treated dendritic cells developed into Th1 cells and generated a strong immune response in lab studies.

Keith Black, MD, director of the Maxine Dunitz Neurosurgical Institute, the Division of Neurosurgery and the Comprehensive Brain Tumor Program, said this is one of several ongoing studies aimed at making the dendritic cell vaccine more effective against the deadliest tumors.

"We are accumulating evidence that brain tumors themselves play a role in suppressing the T cell response, and we think this is one reason gliomas grow so quickly. The strategy of shutting down the IL-10 gene may be one way of counteracting this immune inhibition," said Dr. Black, who holds the Ruth and Lawrence Harvey Chair in Neuroscience at Cedars-Sinai.



The study was supported in part by National Institutes of Health grant number NS02232 to Dr. Yu.

Cedars-Sinai is one of the largest nonprofit academic medical centers in the Western United States. For the fifth straight two-year period, it has been named Southern California’s gold standard in health care in an independent survey. Cedars-Sinai is internationally renowned for its diagnostic and treatment capabilities and its broad spectrum of programs and services, as well as breakthroughs in biomedical research and superlative medical education. It ranks among the top 10 non-university hospitals in the nation for its research activities.

Citation: European Journal of Immunology, June 2004: "Small interference RNA modulation of interleukin 10 in human monocyte-derived dendritic cells enhances the Th1 response."

Sandra Van | Van Communications
Further information:
http://www.csmc.edu/

More articles from Health and Medicine:

nachricht Researchers release the brakes on the immune system
18.10.2017 | Rheinische Friedrich-Wilhelms-Universität Bonn

nachricht Norovirus evades immune system by hiding out in rare gut cells
12.10.2017 | University of Pennsylvania School of Medicine

All articles from Health and Medicine >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Neutron star merger directly observed for the first time

University of Maryland researchers contribute to historic detection of gravitational waves and light created by event

On August 17, 2017, at 12:41:04 UTC, scientists made the first direct observation of a merger between two neutron stars--the dense, collapsed cores that remain...

Im Focus: Breaking: the first light from two neutron stars merging

Seven new papers describe the first-ever detection of light from a gravitational wave source. The event, caused by two neutron stars colliding and merging together, was dubbed GW170817 because it sent ripples through space-time that reached Earth on 2017 August 17. Around the world, hundreds of excited astronomers mobilized quickly and were able to observe the event using numerous telescopes, providing a wealth of new data.

Previous detections of gravitational waves have all involved the merger of two black holes, a feat that won the 2017 Nobel Prize in Physics earlier this month....

Im Focus: Smart sensors for efficient processes

Material defects in end products can quickly result in failures in many areas of industry, and have a massive impact on the safe use of their products. This is why, in the field of quality assurance, intelligent, nondestructive sensor systems play a key role. They allow testing components and parts in a rapid and cost-efficient manner without destroying the actual product or changing its surface. Experts from the Fraunhofer IZFP in Saarbrücken will be presenting two exhibits at the Blechexpo in Stuttgart from 7–10 November 2017 that allow fast, reliable, and automated characterization of materials and detection of defects (Hall 5, Booth 5306).

When quality testing uses time-consuming destructive test methods, it can result in enormous costs due to damaging or destroying the products. And given that...

Im Focus: Cold molecules on collision course

Using a new cooling technique MPQ scientists succeed at observing collisions in a dense beam of cold and slow dipolar molecules.

How do chemical reactions proceed at extremely low temperatures? The answer requires the investigation of molecular samples that are cold, dense, and slow at...

Im Focus: Shrinking the proton again!

Scientists from the Max Planck Institute of Quantum Optics, using high precision laser spectroscopy of atomic hydrogen, confirm the surprisingly small value of the proton radius determined from muonic hydrogen.

It was one of the breakthroughs of the year 2010: Laser spectroscopy of muonic hydrogen resulted in a value for the proton charge radius that was significantly...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

ASEAN Member States discuss the future role of renewable energy

17.10.2017 | Event News

World Health Summit 2017: International experts set the course for the future of Global Health

10.10.2017 | Event News

Climate Engineering Conference 2017 Opens in Berlin

10.10.2017 | Event News

 
Latest News

Terahertz spectroscopy goes nano

20.10.2017 | Information Technology

Strange but true: Turning a material upside down can sometimes make it softer

20.10.2017 | Materials Sciences

NRL clarifies valley polarization for electronic and optoelectronic technologies

20.10.2017 | Interdisciplinary Research

VideoLinks
B2B-VideoLinks
More VideoLinks >>>