Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

New technology blocks gene to increase immune response against deadly brain tumor cells

26.05.2004


With new technology that uses short strands of genetic material to shut down a specific gene, researchers have regulated immune system proteins to boost production of cells that seek and destroy cancer cells. This approach may improve the effectiveness of vaccines in the treatment of tumors, including malignant brain tumors.



Results of the study appear in the June issue of the European Journal of Immunology, and the research was conducted at Cedars-Sinai’s Maxine Dunitz Neurosurgical Institute, where clinical trials of dendritic cell immunotherapy have been underway for several years.

Dendritic cells are the immune system’s most potent antigen-presenting cells – those that identify "foreign" substances for destruction. Because cancer cells often are not recognized by dendritic cells as antigens, the neurosurgeons and other scientists at the Institute have developed and studied a vaccine in the treatment of highly aggressive brain tumors called gliomas. They combine in the laboratory tumor cells that have been surgically removed and dendritic cells derived from a patient’s blood. The new cells are injected back into the patient to seek out other cancer cells for destruction.


Early clinical trials have shown an increase in survival rates among patients receiving the dendritic cell vaccine. Meanwhile, Institute researchers have been studying underlying genetic and cellular mechanisms as well as other methods for increasing immune response and enhancing the vaccine’s effectiveness.

"This study demonstrates that by turning off the interleukin 10 gene in the dendritic cell we can make a much more effective dendritic cell in terms of generating a significant immune response," said John S. Yu, MD, the article’s senior author and co-director of the Comprehensive Brain Tumor Program at Cedars-Sinai.

One of the functions of dendritic cells is to influence immature T cells to become either T helper type 1 (Th1) or T helper type 2 (Th2) cells. A naturally occurring protein, interleukin 12 (IL-12), interacting with dendritic cells, spurs the development of Th1 cells. Interleukin 10 (IL-10) inhibits the production of IL-12.

"Interleukin 10 is a molecule that generates a Th2 response, which is effective against organisms such as bacteria, but for a tumor treatment a Th1 response is our goal. The Th1 response is generated through T cells against a tumor," said Dr. Yu.

In this study, the researchers used a new approach called RNA interference to target the IL-10 gene, inserting short strands of synthetic IL-10 specific RNA (small interfering RNA or siRNA) into dendritic cells generated from peripheral blood cells. Suppression of the IL-10 gene inhibited the secretion of the IL-10 protein, which allowed increased production of IL-12. Naïve T cells co-cultured with siRNA-treated dendritic cells developed into Th1 cells and generated a strong immune response in lab studies.

Keith Black, MD, director of the Maxine Dunitz Neurosurgical Institute, the Division of Neurosurgery and the Comprehensive Brain Tumor Program, said this is one of several ongoing studies aimed at making the dendritic cell vaccine more effective against the deadliest tumors.

"We are accumulating evidence that brain tumors themselves play a role in suppressing the T cell response, and we think this is one reason gliomas grow so quickly. The strategy of shutting down the IL-10 gene may be one way of counteracting this immune inhibition," said Dr. Black, who holds the Ruth and Lawrence Harvey Chair in Neuroscience at Cedars-Sinai.



The study was supported in part by National Institutes of Health grant number NS02232 to Dr. Yu.

Cedars-Sinai is one of the largest nonprofit academic medical centers in the Western United States. For the fifth straight two-year period, it has been named Southern California’s gold standard in health care in an independent survey. Cedars-Sinai is internationally renowned for its diagnostic and treatment capabilities and its broad spectrum of programs and services, as well as breakthroughs in biomedical research and superlative medical education. It ranks among the top 10 non-university hospitals in the nation for its research activities.

Citation: European Journal of Immunology, June 2004: "Small interference RNA modulation of interleukin 10 in human monocyte-derived dendritic cells enhances the Th1 response."

Sandra Van | Van Communications
Further information:
http://www.csmc.edu/

More articles from Health and Medicine:

nachricht Laser activated gold pyramids could deliver drugs, DNA into cells without harm
24.03.2017 | Harvard John A. Paulson School of Engineering and Applied Sciences

nachricht What does congenital Zika syndrome look like?
24.03.2017 | University of California - San Diego

All articles from Health and Medicine >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Giant Magnetic Fields in the Universe

Astronomers from Bonn and Tautenburg in Thuringia (Germany) used the 100-m radio telescope at Effelsberg to observe several galaxy clusters. At the edges of these large accumulations of dark matter, stellar systems (galaxies), hot gas, and charged particles, they found magnetic fields that are exceptionally ordered over distances of many million light years. This makes them the most extended magnetic fields in the universe known so far.

The results will be published on March 22 in the journal „Astronomy & Astrophysics“.

Galaxy clusters are the largest gravitationally bound structures in the universe. With a typical extent of about 10 million light years, i.e. 100 times the...

Im Focus: Tracing down linear ubiquitination

Researchers at the Goethe University Frankfurt, together with partners from the University of Tübingen in Germany and Queen Mary University as well as Francis Crick Institute from London (UK) have developed a novel technology to decipher the secret ubiquitin code.

Ubiquitin is a small protein that can be linked to other cellular proteins, thereby controlling and modulating their functions. The attachment occurs in many...

Im Focus: Perovskite edges can be tuned for optoelectronic performance

Layered 2D material improves efficiency for solar cells and LEDs

In the eternal search for next generation high-efficiency solar cells and LEDs, scientists at Los Alamos National Laboratory and their partners are creating...

Im Focus: Polymer-coated silicon nanosheets as alternative to graphene: A perfect team for nanoelectronics

Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are less stable. Now researchers at the Technical University of Munich (TUM) have, for the first time ever, produced a composite material combining silicon nanosheets and a polymer that is both UV-resistant and easy to process. This brings the scientists a significant step closer to industrial applications like flexible displays and photosensors.

Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are...

Im Focus: Researchers Imitate Molecular Crowding in Cells

Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to simulate these confined natural conditions in artificial vesicles for the first time. As reported in the academic journal Small, the results are offering better insight into the development of nanoreactors and artificial organelles.

Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

International Land Use Symposium ILUS 2017: Call for Abstracts and Registration open

20.03.2017 | Event News

CONNECT 2017: International congress on connective tissue

14.03.2017 | Event News

ICTM Conference: Turbine Construction between Big Data and Additive Manufacturing

07.03.2017 | Event News

 
Latest News

Argon is not the 'dope' for metallic hydrogen

24.03.2017 | Materials Sciences

Astronomers find unexpected, dust-obscured star formation in distant galaxy

24.03.2017 | Physics and Astronomy

Gravitational wave kicks monster black hole out of galactic core

24.03.2017 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>