Radiologists provide safe delivery method for gene therapy
Computed tomography (CT)-guided injections offer a safe delivery method for gene therapy in patients with metastatic kidney cancer, according to a study in the May issue of the journal Radiology.
Gene therapy involves introducing genetic material directly into cells to fight disease. "The new gene therapies offer promise for controlling certain types of cancer, but delivering the agents directly into tumors poses its own set of challenges," said the studys lead author, Robert D. Suh, M.D., who is an assistant clinical professor of radiology and director of thoracic interventional services at the David Geffen School of Medicine at University of California, Los Angeles (UCLA). "As research in gene therapy, or immunotherapy, progresses, we need a good gene therapy delivery mechanism."
Treatment of metastatic kidney cancer is difficult because the disease is largely resistant to chemotherapy. The only Food and Drug Administration (FDA)-approved immunotherapeutic treatment, recombinant interleukin-2 (IL-2), has a response rate of only 15 percent when administered intravenously, and its use is limited by significant and occasionally life-threatening side effects. Researchers have developed several gene therapy agents to improve the effectiveness and minimize the side effects of IL-2. UCLAs technique involves injecting an IL-2-encoded recombinant gene directly into cancer cells.
The researchers assessed the feasibility of using CT guidance to safely position a needle in both superficial and deep tumor sites to deliver gene therapy (IL-2 plasmid DNA).
"The CT images enabled us to precisely target the tumor, eliminating any guesswork about where to angle the needle and how deeply to inject the therapy," said Dr. Suh.
Twenty-nine patients with kidney cancer that had spread to the chest or abdomen received up to three cycles of six weekly injections directly into their tumors. A total of 284 CT-guided injections were performed.
In every case, the UCLA team successfully placed the needle and injected the agent into the tumor. None of the patients experienced serious adverse effects. Minor complications occurred in 14.8 percent of injections. The most common complication was air collection in the chest, for which only one patient required drainage. The complication rate did not increase with the number of injections.
"Our findings validate that CT-guided injection and delivery of gene therapy agents is both feasible and safe," said Dr. Suh.
"Metastatic Renal Cell Carcinoma: CT-guided Immunotherapy as a Technically Feasible and Safe Approach to Delivery of Gene Therapy for Treatment." Collaborating with Dr. Suh on this paper were Jonathan G. Goldin, M.D., Amanda B. Wallace, B.S., Ramon E. Sheehan, M.D., Stefan B. Heinze, M.D., Barbara J. Gitlitz, M.D., and Robert A. Figlin, M.D.
Maureen Morley | EurekAlert!
The most recent press releases about innovation >>>
Die letzten 5 Focus-News des innovations-reports im Überblick:
Whether you call it effervescent, fizzy, or sparkling, carbonated water is making a comeback as a beverage. Aside from quenching thirst, researchers at the University of Illinois at Urbana-Champaign have discovered a new use for these "bubbly" concoctions that will have major impact on the manufacturer of the world's thinnest, flattest, and one most useful materials -- graphene.
As graphene's popularity grows as an advanced "wonder" material, the speed and quality at which it can be manufactured will be paramount. With that in mind,...
Physicists at the University of Bonn have managed to create optical hollows and more complex patterns into which the light of a Bose-Einstein condensate flows. The creation of such highly low-loss structures for light is a prerequisite for complex light circuits, such as for quantum information processing for a new generation of computers. The researchers are now presenting their results in the journal Nature Photonics.
Light particles (photons) occur as tiny, indivisible portions. Many thousands of these light portions can be merged to form a single super-photon if they are...
For the first time, scientists have shown that circular RNA is linked to brain function. When a RNA molecule called Cdr1as was deleted from the genome of mice, the animals had problems filtering out unnecessary information – like patients suffering from neuropsychiatric disorders.
While hundreds of circular RNAs (circRNAs) are abundant in mammalian brains, one big question has remained unanswered: What are they actually good for? In the...
An experimental small satellite has successfully collected and delivered data on a key measurement for predicting changes in Earth's climate.
The Radiometer Assessment using Vertically Aligned Nanotubes (RAVAN) CubeSat was launched into low-Earth orbit on Nov. 11, 2016, in order to test new...
A study led by scientists of the Max Planck Institute for the Structure and Dynamics of Matter (MPSD) at the Center for Free-Electron Laser Science in Hamburg presents evidence of the coexistence of superconductivity and “charge-density-waves” in compounds of the poorly-studied family of bismuthates. This observation opens up new perspectives for a deeper understanding of the phenomenon of high-temperature superconductivity, a topic which is at the core of condensed matter research since more than 30 years. The paper by Nicoletti et al has been published in the PNAS.
Since the beginning of the 20th century, superconductivity had been observed in some metals at temperatures only a few degrees above the absolute zero (minus...