Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

New more rapid methods for the detection of salmonella

22.04.2004


The food and drink we consume have to pass strict quality control tests. Nevertheless, these precautions are not always sufficient, given that some foodstuffs still give rise to illness. In most cases, food poisoning is caused by micro-organisms. The salmonella bacteria is, without doubt, one of the better known ones. The University of the Basque Country (EHU) is developing a new system to detect salmonella with greater rapidity - within 24 hours.



Salmonella is quite a ubiquitous bacteria, found in foodstuffs of animal origin and in contaminated water. It is a resistant micro-organism which adapts easily to extreme environmental conditions. Salmonella actively grows under a wide range of temperatures: less than or equal to 54 degrees Celsius. The consequences are well known to all: enterocolitis, systemic infections, gastro-enteritis and typhoid fever.

Nowadays, detecting salmonella in food is a simple process. An analysis is carried out in the laboratory, by means of conventional microbiological culture growth techniques and the results are obtained within 7 days.


Nevertheless, this gives rise to problems for doctors or for the food industry, not being able to afford this time period and so often finding that they have to make decisions without waiting for or depending on the analysis results. At the Department of Immunology, Microbiology and Parasitology of the EHU, it was realised that there was a need to develop more rapid detection systems and, thus, research in the field of genetic techniques was instigated. To this end, contact was made with the company in Alava (in the Basque Country), Laboratorios Bromatológicos Araba.

Three bodies are working jointly on this project: the EHU, responsible for the development of a detection system for viable bacteria; Laboratorios Bromatológicos Araba, which applies the university research results to real samples and, finally, the LEIA Technological Centre, charged with developing pre-treatment systems for the samples. The aim of the project is to manage to detect salmonella infection within 24 hours.

Moreover, the fact that the complete genome for salmonella – some 4,500,000 pairs of bases - became known only a few years ago, has provided a great advance for researchers. Although it is too early to talk of results, it has been demonstrated that just a DNA extraction is not sufficient in order to detect salmonella, given that it does not indicate whether or not the bacteria is alive or dead. So, with new genetic techniques, other, more specific markers for the viability of the bacteria are being sought. One of these could be RNA.

It is the specific genes just of the salmonella that is the target of this research – some 100 or 200 genes. To this end, a new device that functions much more rapidly has been acquired a PCR which works in real time. This device, moreover, enables the quantifying of the reaction, i.e. it tells us the number of species of salmonella there is. The PCR results are analysed through various graphical representations and then interpreted. The green line indicates the presence of salmonella.

Nerea Pikabea | Basque research
Further information:
http://www.ehu.es
http://www.basqueresearch.com/berria_irakurri.asp?Gelaxka=1_1&Berri_Kod=450&hizk=I

More articles from Health and Medicine:

nachricht Biofilm discovery suggests new way to prevent dangerous infections
23.05.2017 | University of Texas at Austin

nachricht Another reason to exercise: Burning bone fat -- a key to better bone health
19.05.2017 | University of North Carolina Health Care

All articles from Health and Medicine >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Can the immune system be boosted against Staphylococcus aureus by delivery of messenger RNA?

Staphylococcus aureus is a feared pathogen (MRSA, multi-resistant S. aureus) due to frequent resistances against many antibiotics, especially in hospital infections. Researchers at the Paul-Ehrlich-Institut have identified immunological processes that prevent a successful immune response directed against the pathogenic agent. The delivery of bacterial proteins with RNA adjuvant or messenger RNA (mRNA) into immune cells allows the re-direction of the immune response towards an active defense against S. aureus. This could be of significant importance for the development of an effective vaccine. PLOS Pathogens has published these research results online on 25 May 2017.

Staphylococcus aureus (S. aureus) is a bacterium that colonizes by far more than half of the skin and the mucosa of adults, usually without causing infections....

Im Focus: A quantum walk of photons

Physicists from the University of Würzburg are capable of generating identical looking single light particles at the push of a button. Two new studies now demonstrate the potential this method holds.

The quantum computer has fuelled the imagination of scientists for decades: It is based on fundamentally different phenomena than a conventional computer....

Im Focus: Turmoil in sluggish electrons’ existence

An international team of physicists has monitored the scattering behaviour of electrons in a non-conducting material in real-time. Their insights could be beneficial for radiotherapy.

We can refer to electrons in non-conducting materials as ‘sluggish’. Typically, they remain fixed in a location, deep inside an atomic composite. It is hence...

Im Focus: Wafer-thin Magnetic Materials Developed for Future Quantum Technologies

Two-dimensional magnetic structures are regarded as a promising material for new types of data storage, since the magnetic properties of individual molecular building blocks can be investigated and modified. For the first time, researchers have now produced a wafer-thin ferrimagnet, in which molecules with different magnetic centers arrange themselves on a gold surface to form a checkerboard pattern. Scientists at the Swiss Nanoscience Institute at the University of Basel and the Paul Scherrer Institute published their findings in the journal Nature Communications.

Ferrimagnets are composed of two centers which are magnetized at different strengths and point in opposing directions. Two-dimensional, quasi-flat ferrimagnets...

Im Focus: World's thinnest hologram paves path to new 3-D world

Nano-hologram paves way for integration of 3-D holography into everyday electronics

An Australian-Chinese research team has created the world's thinnest hologram, paving the way towards the integration of 3D holography into everyday...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Marine Conservation: IASS Contributes to UN Ocean Conference in New York on 5-9 June

24.05.2017 | Event News

AWK Aachen Machine Tool Colloquium 2017: Internet of Production for Agile Enterprises

23.05.2017 | Event News

Dortmund MST Conference presents Individualized Healthcare Solutions with micro and nanotechnology

22.05.2017 | Event News

 
Latest News

How herpesviruses win the footrace against the immune system

26.05.2017 | Life Sciences

Water forms 'spine of hydration' around DNA, group finds

26.05.2017 | Life Sciences

First Juno science results supported by University of Leicester's Jupiter 'forecast'

26.05.2017 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>