Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

The structure of a key enzyme for infectious diseases solved at ESRF

12.08.2003


The molecular structure and shape of CDP-ME kinase


The shape of the CDP-ME kinase active site with location of substrates.


A European team of scientists from the University of Dundee (UK), the Technical University of Munich (Germany) and the European Synchrotron Radiation Facility, ESRF, (France) have determined the structure of a key target enzyme for novel drug development to treat infectious diseases including malaria, tuberculosis and sexually transmitted bacterial infections. The results of their collaboration are published on the August 5 issue of Proceedings of the National Academy of Sciences.

Synchrotron radiation at the ESRF played a key role in the determination of the structure of the enzyme CDP-ME kinase. The experiment took place on one of the macromolecular crystallography beamlines at the ESRF. This kinase helps to produce many of the molecules that bacteria and parasites need to live and multiply. A molecule that can prevent the kinase from working normally will poison and kill the pathogenic organisms.

The determination of the structure of the enzyme provides a template for the design of small molecules that will inhibit its action and prevent it from working normally. In the future, the structure may help lead to the development of new potent therapies for a wide range of microbial infections. “These drugs could potentially help the treatment of not only malaria and tuberculosis but also toxoplasmosis, chlamydia, meningitis and cholera for example”, explains Professor Bill Hunter, one of the authors of the article.



About the technique

Protein crystallography consists of the production of highly pure protein samples. These samples are then crystallised to produce single crystals of the protein. When X-rays interact with single crystals, the interaction between the constituents of the crystal and the X-rays results in the production of an interference pattern – known as a diffraction pattern. By the use of suitable physical and chemical techniques it is possible to use the diffraction patterns in order to determine the spatial distribution of the electrons within the crystal sample. The chemical interpretation of this produces the structural model that reveals the three-dimensional structure of the protein.

The ESRF presents substantial improvements for the exploitation of this technique; in particular the use of intense, highly collimated X-ray beams greatly aids the investigation of small weakly diffracting crystals.

Montserrat Capellas | ESRF
Further information:
http://www.esrf.fr/News/FrontNews/PressRelease_11_08_2003/

More articles from Health and Medicine:

nachricht NIST scientists discover how to switch liver cancer cell growth from 2-D to 3-D structures
17.11.2017 | National Institute of Standards and Technology (NIST)

nachricht High speed video recording precisely measures blood cell velocity
15.11.2017 | ITMO University

All articles from Health and Medicine >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: A “cosmic snake” reveals the structure of remote galaxies

The formation of stars in distant galaxies is still largely unexplored. For the first time, astron-omers at the University of Geneva have now been able to closely observe a star system six billion light-years away. In doing so, they are confirming earlier simulations made by the University of Zurich. One special effect is made possible by the multiple reflections of images that run through the cosmos like a snake.

Today, astronomers have a pretty accurate idea of how stars were formed in the recent cosmic past. But do these laws also apply to older galaxies? For around a...

Im Focus: Visual intelligence is not the same as IQ

Just because someone is smart and well-motivated doesn't mean he or she can learn the visual skills needed to excel at tasks like matching fingerprints, interpreting medical X-rays, keeping track of aircraft on radar displays or forensic face matching.

That is the implication of a new study which shows for the first time that there is a broad range of differences in people's visual ability and that these...

Im Focus: Novel Nano-CT device creates high-resolution 3D-X-rays of tiny velvet worm legs

Computer Tomography (CT) is a standard procedure in hospitals, but so far, the technology has not been suitable for imaging extremely small objects. In PNAS, a team from the Technical University of Munich (TUM) describes a Nano-CT device that creates three-dimensional x-ray images at resolutions up to 100 nanometers. The first test application: Together with colleagues from the University of Kassel and Helmholtz-Zentrum Geesthacht the researchers analyzed the locomotory system of a velvet worm.

During a CT analysis, the object under investigation is x-rayed and a detector measures the respective amount of radiation absorbed from various angles....

Im Focus: Researchers Develop Data Bus for Quantum Computer

The quantum world is fragile; error correction codes are needed to protect the information stored in a quantum object from the deteriorating effects of noise. Quantum physicists in Innsbruck have developed a protocol to pass quantum information between differently encoded building blocks of a future quantum computer, such as processors and memories. Scientists may use this protocol in the future to build a data bus for quantum computers. The researchers have published their work in the journal Nature Communications.

Future quantum computers will be able to solve problems where conventional computers fail today. We are still far away from any large-scale implementation,...

Im Focus: Wrinkles give heat a jolt in pillared graphene

Rice University researchers test 3-D carbon nanostructures' thermal transport abilities

Pillared graphene would transfer heat better if the theoretical material had a few asymmetric junctions that caused wrinkles, according to Rice University...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Ecology Across Borders: International conference brings together 1,500 ecologists

15.11.2017 | Event News

Road into laboratory: Users discuss biaxial fatigue-testing for car and truck wheel

15.11.2017 | Event News

#Berlin5GWeek: The right network for Industry 4.0

30.10.2017 | Event News

 
Latest News

NASA detects solar flare pulses at Sun and Earth

17.11.2017 | Physics and Astronomy

NIST scientists discover how to switch liver cancer cell growth from 2-D to 3-D structures

17.11.2017 | Health and Medicine

The importance of biodiversity in forests could increase due to climate change

17.11.2017 | Studies and Analyses

VideoLinks
B2B-VideoLinks
More VideoLinks >>>