Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

The Trojan Clot-Buster: Drug-Coated Red Blood Cells Destroy Blood Clots From Within

12.08.2003


Thrombosis - the formation of internal blood clots - is a common cause of complications and even death following surgery. To create a better means of preventing thrombosis, researchers at University of Pennsylvania School of Medicine coated red blood cells (RBCs) with tissue plasminogen activator (tPA), a clot-dissolving drug commonly used as an emergency treatment for stroke. When given alone, tPA has a short life span in circulation and has the potential to cause serious bleeding as it diffuses out of the bloodstream. The RBC/tPA combo, however, lasts ten times longer in the bloodstream than free-floating tPA and decreases the likelihood of excess bleeding, according to a new study.



"The idea of coating red blood cells with tPA was to create a Trojan Horse, a vehicle for sneaking tPA into the bloodstream that could not only add to the drug’s longevity, but would also allow it to be incorporated into a growing blood clot. RBC/tPA can dissolve blood clots from within," said Vladimir R, Muzykantov, MD, PhD, associate professor in Penn’s Department of Pharmacology and author of the study. "Our research shows that the Trojan Horse approach converts tPA into a potent killer of nascent blood clots, one that would pose a much smaller risk of causing internal bleeding."

In the August issue of Nature Biotechnology, Muzykantov and his colleagues demonstrate in animal models how the marriage of red blood cells and tPA has the potential of safely preventing thrombosis following surgery and as a therapeutic for victims of heart attack or stoke.


"If developed for humans, the RBC/tPA method could provide an ideal way of delivering clot-busting drugs, with fewer side effects," said Muzykantov. "In theory, patients could donate blood before surgery and receive their own cells bound to tPA following surgery, providing a safer alternative to blood-thinning medication."

Research has shown that preventing thrombosis helps to reduce mortality and morbidity in many diseases. Unfortunately, current clot-busting drugs have the tendency to cause excessive bleeding, either by causing bleeding outside of the blood vessels or by removing pre-existing and, perhaps, beneficial blood clots. According to the Penn researchers, RCB/tPA spares existing blood clots and is too large to cause damage outside of the bloodstream.

To coat red blood cells with tPA, Muzykantov and his colleagues capitalized on the ’stickiness’ of streptavidin-biotin, a protein complex used in laboratories to study molecular interactions. Streptavidin forms an incredibly tight bond to a tiny molecule called biotin, so the researchers ’biotinylated’ tPA and RBCs and used streptavidin to link them together. According to the researchers, the technique may provide a safe way of extending the longevity and safety of drugs within the circulatory system.

"Red blood cells can travel hundreds of kilometers throughout the blood vessels during their 100-or so day life-span. That fact alone makes the idea of RBC-bound therapeutics very interesting," said Muzykantov. "Moreover, red blood cells are relatively large, which makes it very difficult for drugs bound to them to burrow their way out of the bloodstream where they could potentially do damage."

Greg Lester | University of Pennsylvania
Further information:
http://www.uphs.upenn.edu/news/News_Releases/august03/clotbuster.htm

More articles from Health and Medicine:

nachricht Researchers release the brakes on the immune system
18.10.2017 | Rheinische Friedrich-Wilhelms-Universität Bonn

nachricht Norovirus evades immune system by hiding out in rare gut cells
12.10.2017 | University of Pennsylvania School of Medicine

All articles from Health and Medicine >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Neutron star merger directly observed for the first time

University of Maryland researchers contribute to historic detection of gravitational waves and light created by event

On August 17, 2017, at 12:41:04 UTC, scientists made the first direct observation of a merger between two neutron stars--the dense, collapsed cores that remain...

Im Focus: Breaking: the first light from two neutron stars merging

Seven new papers describe the first-ever detection of light from a gravitational wave source. The event, caused by two neutron stars colliding and merging together, was dubbed GW170817 because it sent ripples through space-time that reached Earth on 2017 August 17. Around the world, hundreds of excited astronomers mobilized quickly and were able to observe the event using numerous telescopes, providing a wealth of new data.

Previous detections of gravitational waves have all involved the merger of two black holes, a feat that won the 2017 Nobel Prize in Physics earlier this month....

Im Focus: Smart sensors for efficient processes

Material defects in end products can quickly result in failures in many areas of industry, and have a massive impact on the safe use of their products. This is why, in the field of quality assurance, intelligent, nondestructive sensor systems play a key role. They allow testing components and parts in a rapid and cost-efficient manner without destroying the actual product or changing its surface. Experts from the Fraunhofer IZFP in Saarbrücken will be presenting two exhibits at the Blechexpo in Stuttgart from 7–10 November 2017 that allow fast, reliable, and automated characterization of materials and detection of defects (Hall 5, Booth 5306).

When quality testing uses time-consuming destructive test methods, it can result in enormous costs due to damaging or destroying the products. And given that...

Im Focus: Cold molecules on collision course

Using a new cooling technique MPQ scientists succeed at observing collisions in a dense beam of cold and slow dipolar molecules.

How do chemical reactions proceed at extremely low temperatures? The answer requires the investigation of molecular samples that are cold, dense, and slow at...

Im Focus: Shrinking the proton again!

Scientists from the Max Planck Institute of Quantum Optics, using high precision laser spectroscopy of atomic hydrogen, confirm the surprisingly small value of the proton radius determined from muonic hydrogen.

It was one of the breakthroughs of the year 2010: Laser spectroscopy of muonic hydrogen resulted in a value for the proton charge radius that was significantly...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

ASEAN Member States discuss the future role of renewable energy

17.10.2017 | Event News

World Health Summit 2017: International experts set the course for the future of Global Health

10.10.2017 | Event News

Climate Engineering Conference 2017 Opens in Berlin

10.10.2017 | Event News

 
Latest News

Terahertz spectroscopy goes nano

20.10.2017 | Information Technology

Strange but true: Turning a material upside down can sometimes make it softer

20.10.2017 | Materials Sciences

NRL clarifies valley polarization for electronic and optoelectronic technologies

20.10.2017 | Interdisciplinary Research

VideoLinks
B2B-VideoLinks
More VideoLinks >>>