Figure 1: Ligand fishing (BIA-MS coupling)
Figure 2: ADME assays of small compounds binding to HSA
Biomolecular interaction analysis (BIA) using SPR (surface plasmon resonance) biosensors is now utilised increasingly in nearly all phases of drug development. The BIA system consists out of a light source emitting near infrared light, a sensor microchip, an automated liquid handling system with constant flow and a diode array position-sensitive detector. One of the two interacting partners (referred to as the ligand) is immobilized on the sensor surface. The other binding partner, called the analyte, is directed over the surface in a constant flow system allowing to monitor the interaction of the binding partners in “real time”.
BIA covers a broad range of applications in target identification and validation, secondary screens and lead optimisation, early ADME assays as well as testing immunoreactivity.
Assay development and functional assays of proteins are the most commonly used areas of applications of BIA in drug development.
Dr. Stephan Drewianka | Biaffin GmbH & Co KG
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