Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

New Research Establishes Genetic Associations In Hepatitis C

10.04.2003


Hepatitis C Virus (HCV), as a major agent of non-A, non-B hepatitis (NANBH), has been described as an insidious disease and a silent epidemic, mainly because the infection is often sub-clinical. Acute infection is recognized in a minority of patients and in most cases the virus results in chronic infections taking 10-20 years before the emergence of liver disease. In the US, almost four million individuals are infected and up to 170 million worldwide.



Background

An imbalance in helper T-cell type-1 (Th1) and type-2 (Th2) cytokines has been suggested as playing an important role in the cause of chronic viral infections, but this issue is not resolved in patients with hepatitis C virus (HCV) infection. Inflammation of the portal and portal areas is a common feature of chronic hepatitis C. Antigen presenting dendritic, or “tree-like,” cells are located in the portal area, and infiltrating T-cells are initially exposed to infected hepatocytes in the peripheral area. Thus, these areas could be sites of the initial processes of the immune response in chronic hepatitis C.


The pathogenesis of hepatitis C virus associated liver injury involves many genes from multiple pathogenic pathways. Blockade of CC-chemokine receptor 5 (CCR5) has been proposed as therapy for HIV-1. Therefore, an examination was conducted to determine if the CCR5-delta32 homozygous genotype has phenotypic expression other than those related to HIV-1. Other genes associated with viral infection (RANTES, CD4), and in inflammation (Interleukin1B) were also reviewed for their possible association.

Hepatitis C virus (HCV) is commonly seen in the Coachella Valley of Southern California. Genetic Research institute of the Desert (GRID), a nonprofit research center was established in the Coachella valley with a goal to investigate genetic aspects of cancer, as well as infectious and some neurological diseases. This research effort focused on the genetic variants in the HCV group of patients compared to normal healthy individuals. Genetic association could determine the drug responsiveness and aid in treatment; accordingly, a research objective was to determine genetic variants associated with the disease and to find the multiple allelic associations of these genes. The associated genes can be used as prognostic markers, as well as help design drug for hepatitis.

A New Study

The authors of “Multiple Gene Associations In Hepatitis ‘C’ Infection, ” are Radhika Gade-Andavolu, PhD from Genetic Research Institute of the Desert, Rancho Mirage, Gary Annunziata, DO, and Lawrence A. Cone, MD, DSc, are from the Eisenhower Medical Center, Rancho Mirage, CA. Their findings are being presented at Experimental Biology 2003, a meeting sponsored by the American Physiological Society, being held April 11-15, 2003, at the San Diego Convention Center, San Diego, CA.

Methodology

Patients seeking treatment at Infectious Diseases and Gastroenterology clinics

at the Eisenhower Medical center participated in this research. After informed consent was obtained blood was drawn from patients; DNA was isolated from the blood and used for analysis. Genomic DNA from 26 hepatitis C patients and 318 healthy controls was subjected for PCR analysis to determine the genotypes of four genes.

Results

In this study, the frequency of the SNP’s of IL1B and RANTES, a 32bp deletion in CCR5 and CD4 pentanucleotide repeat polymorphisms was evaluated. The findings were reviewed for association of various genotypes with respect to disease onset and severity scores, and a statistical analysis was done.

Out of the four genes studied in patients, only CD4 repeat (p= 0.006) was highly significant with respect to controls. With the onset of disease relative to age, CD4 (p= 0.02) and IL-B (p= 0.012) were significant, while CCR5 delta 32mutation showed significance with severity score (p=0.08). IL-1B and RANTES genes showed positive correlation (p= 0.04) with pair wise bivariate analysis.

Conclusions

These preliminary findings with CD4 indicate a correlation between dominant Th1 response and disease activity and progression suggesting a possible role of intrahepatic CD4 T cells in hepatic injury of HCV infection. In addition the findings of CCR5, IL1B and its significant correlation with RANTES suggests their possible roles in the immune response to HCV infection in and around the portal area by attracting naïve and active T-cells. This research will continue with the addition of more genes and the target set numbers of genes are to be pooled to ascertain the risk.

Donna Krupa | American Physiological Society
Further information:
http://www.the-aps.org/press_room/eb03/7.htm

More articles from Health and Medicine:

nachricht Usher syndrome: Gene therapy restores hearing and balance
25.09.2017 | Institut Pasteur

nachricht MRI contrast agent locates and distinguishes aggressive from slow-growing breast cancer
25.09.2017 | Case Western Reserve University

All articles from Health and Medicine >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: The fastest light-driven current source

Controlling electronic current is essential to modern electronics, as data and signals are transferred by streams of electrons which are controlled at high speed. Demands on transmission speeds are also increasing as technology develops. Scientists from the Chair of Laser Physics and the Chair of Applied Physics at Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU) have succeeded in switching on a current with a desired direction in graphene using a single laser pulse within a femtosecond ¬¬ – a femtosecond corresponds to the millionth part of a billionth of a second. This is more than a thousand times faster compared to the most efficient transistors today.

Graphene is up to the job

Im Focus: LaserTAB: More efficient and precise contacts thanks to human-robot collaboration

At the productronica trade fair in Munich this November, the Fraunhofer Institute for Laser Technology ILT will be presenting Laser-Based Tape-Automated Bonding, LaserTAB for short. The experts from Aachen will be demonstrating how new battery cells and power electronics can be micro-welded more efficiently and precisely than ever before thanks to new optics and robot support.

Fraunhofer ILT from Aachen relies on a clever combination of robotics and a laser scanner with new optics as well as process monitoring, which it has developed...

Im Focus: The pyrenoid is a carbon-fixing liquid droplet

Plants and algae use the enzyme Rubisco to fix carbon dioxide, removing it from the atmosphere and converting it into biomass. Algae have figured out a way to increase the efficiency of carbon fixation. They gather most of their Rubisco into a ball-shaped microcompartment called the pyrenoid, which they flood with a high local concentration of carbon dioxide. A team of scientists at Princeton University, the Carnegie Institution for Science, Stanford University and the Max Plank Institute of Biochemistry have unravelled the mysteries of how the pyrenoid is assembled. These insights can help to engineer crops that remove more carbon dioxide from the atmosphere while producing more food.

A warming planet

Im Focus: Highly precise wiring in the Cerebral Cortex

Our brains house extremely complex neuronal circuits, whose detailed structures are still largely unknown. This is especially true for the so-called cerebral cortex of mammals, where among other things vision, thoughts or spatial orientation are being computed. Here the rules by which nerve cells are connected to each other are only partly understood. A team of scientists around Moritz Helmstaedter at the Frankfiurt Max Planck Institute for Brain Research and Helene Schmidt (Humboldt University in Berlin) have now discovered a surprisingly precise nerve cell connectivity pattern in the part of the cerebral cortex that is responsible for orienting the individual animal or human in space.

The researchers report online in Nature (Schmidt et al., 2017. Axonal synapse sorting in medial entorhinal cortex, DOI: 10.1038/nature24005) that synapses in...

Im Focus: Tiny lasers from a gallery of whispers

New technique promises tunable laser devices

Whispering gallery mode (WGM) resonators are used to make tiny micro-lasers, sensors, switches, routers and other devices. These tiny structures rely on a...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

“Lasers in Composites Symposium” in Aachen – from Science to Application

19.09.2017 | Event News

I-ESA 2018 – Call for Papers

12.09.2017 | Event News

EMBO at Basel Life, a new conference on current and emerging life science research

06.09.2017 | Event News

 
Latest News

Bacterial Nanosized Speargun Works Like a Power Drill

26.09.2017 | Life Sciences

The fastest light-driven current source

26.09.2017 | Physics and Astronomy

Beer can lift your spirits

26.09.2017 | Life Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>