The mature heart is composed by different cell types, including contractile cardiac cells, vascular cells, smooth muscle cells as well as pacemaker cells. During embryonic development as well as during embryonic stem cell differentiation, the different cardiovascular cell types arise from the differentiation of multipotent cardiovascular progenitors. The mechanism that promotes multipotent cardiovascular progenitor specification from undifferentiated mesoderm cell remains largely unknown.
Now, researchers from the Université Libre de Bruxelles lead by Cédric Blanpain (IRIBHM, Faculty of Medicine), shed new light into the early mechanism that governs cardiovascular specification. This research is published in July 3 issue of Cell Stem Cell.
Researchers uncover the key molecular switch that specifies undifferentiated mesodermal cells to become cardiovascular progenitors. The authors of this study found that a protein called Mesp1 acts a master regulator of multipotent cardiovascular progenitor specification. They showed that a very transient expression of a Mesp1, increase by more than 500% the differentiation of pluripotent stem cells into cardiac and vascular cells, which represent the greatest promotion of cardiovascular differentiation induced by a single factor. “When we look at the Mesp1 stimulated cells under the microscope, it was just amazing! It was looking like all cells became cardiac cells, and were spontaneously beating everywhere in the dish”, comments Antoine Bondue, the first author of the paper.
To better understand the molecular mechanism by which Mesp1 promotes cardiovascular specification, researchers from ULB used a genome-wide analysis to identify which genes are regulated by Mesp1. They found that Mesp1 directly activated many previously identified key genes responsible for cardiovascular differentiation. “Mesp1 allows the coordinate expression of all these important cardiac genes at the right place and at the right time”, comments Cédric Blanpain, the principal investigator of this study. Mesp1 also directly repressed genes promoting the acquisition of other possible cell identity during this developmental stage, ensuring the specificity in the promotion of cardiovascular cell identity induced by Mesp1. These results demonstrate that Mesp1 acts as a key regulatory switch during cardiovascular specification, residing at the top of the hierarchy of the gene network responsible for cardiovascular cell fate determination.
This new and exciting study from Cédric Blanpain Lab has also important clinical and pharmaceutical implications. Cardiovascular diseases are the primary cause of death in western countries. The method presented in this study may be used in the future to increase the source of cardiovascular cells for cellular therapy in humans, but also to generate the large amount of cardiovascular cells required for toxicology and drug screenings.
Nancy Dath | alfa
Oxygen can wake up dormant bacteria for antibiotic attacks
08.12.2016 | Penn State
NTU scientists build new ultrasound device using 3-D printing technology
07.12.2016 | Nanyang Technological University
In recent years, lasers with ultrashort pulses (USP) down to the femtosecond range have become established on an industrial scale. They could advance some applications with the much-lauded “cold ablation” – if that meant they would then achieve more throughput. A new generation of process engineering that will address this issue in particular will be discussed at the “4th UKP Workshop – Ultrafast Laser Technology” in April 2017.
Even back in the 1990s, scientists were comparing materials processing with nanosecond, picosecond and femtosesecond pulses. The result was surprising:...
Have you ever wondered how you see the world? Vision is about photons of light, which are packets of energy, interacting with the atoms or molecules in what...
A multi-institutional research collaboration has created a novel approach for fabricating three-dimensional micro-optics through the shape-defined formation of porous silicon (PSi), with broad impacts in integrated optoelectronics, imaging, and photovoltaics.
Working with colleagues at Stanford and The Dow Chemical Company, researchers at the University of Illinois at Urbana-Champaign fabricated 3-D birefringent...
In experiments with magnetic atoms conducted at extremely low temperatures, scientists have demonstrated a unique phase of matter: The atoms form a new type of quantum liquid or quantum droplet state. These so called quantum droplets may preserve their form in absence of external confinement because of quantum effects. The joint team of experimental physicists from Innsbruck and theoretical physicists from Hannover report on their findings in the journal Physical Review X.
“Our Quantum droplets are in the gas phase but they still drop like a rock,” explains experimental physicist Francesca Ferlaino when talking about the...
The Max Planck Institute for Physics (MPP) is opening up a new research field. A workshop from November 21 - 22, 2016 will mark the start of activities for an innovative axion experiment. Axions are still only purely hypothetical particles. Their detection could solve two fundamental problems in particle physics: What dark matter consists of and why it has not yet been possible to directly observe a CP violation for the strong interaction.
The “MADMAX” project is the MPP’s commitment to axion research. Axions are so far only a theoretical prediction and are difficult to detect: on the one hand,...
16.11.2016 | Event News
01.11.2016 | Event News
14.10.2016 | Event News
08.12.2016 | Life Sciences
08.12.2016 | Physics and Astronomy
08.12.2016 | Materials Sciences