Further study may help make biomedical devices safer and explain urinary tract infections
The Cell paper shows how force stretches FimH. The active site is green, and the force stretches the segment that connects to the rest of the bacteria, in pink.
Researchers at the University of Washington have learned that something most people take for granted is not true: that the force of fluids within the human body helps to break the adhesive bonds of invasive bacteria and counterbalance infection.
Most scientists as well as lay people assume, for example, that a sneeze helps clear infection, or that urine helps to clear bacteria from the urinary tract.
"We need to know how bacterial adhesion is altered by shear," says another author, Dr. Viola Vogel, director of the University of Washingtons Center for Nanotechnology in the Department of Bioengineering. "The most amazing part of this is that conventional wisdom says that bacteria have a more difficult time adhering to surfaces when they are subjected to shear force – whether the bacteria are in the intestines, in the urinary tract or in biomedical implants. This paper explains how bacteria firmly adhere to surfaces under shear flow, which is remarkable."
Other authors of the paper include Wendy E. Thomas, of the UW Department of Bioengineering, Dr. Elena Trintchina of the Department of Microbiology and Manu Forero of the Department of Physics.
"This is a fairly startling concept," says Dr. Harry L.T. Mobley, professor of microbiology and immunology at the University of Maryland School of Medicine. He is not an author of the paper. "This describes a protein that sticks to things. Usually, we think of a protein either sticking to something or not sticking to something. Here we see a protein binding tighter when it is trying to be sheared off. That opens the door to further investigation."
Presently, most research operates on the assumption that shear stress reduces the lifetime of a receptor bond. However, the paper in Cell suggests that the force might be exactly what it takes to get the bacteria to adhere.
"Bacterial adhesion has been described for a century – bacteria need to adhere in order to colonize," Sokurenko says. "Its taken a century before weve been able to understand what happens once you see the bacteria clump red blood cells. What happens is that the bacteria and blood cells start to separate after you stop shaking. Then, if you shake them again, they clump again. The moment shear starts pushing them away from the surface, the bacteria adhere tightly. It demonstrates an amazing flexibility by infectious bacteria and provides a mechanism for bacteria to resist the effects of free-flowing inhibitor molecules that can block the adhesion."
In other words, E. coli appears designed to colonize parts of the body that are exposed to a lot of shear force. It has hair-like protrusions, fimbriae, (with the FimH protein on their tips) that touch the nearby surface, detect the dragging force, and set off a chain of molecular events that cause it to cling more effectively.
The computationally derived insights of how the switch works were tested by using genetic approaches to change individual amino acids on FimH. Thomas and Vogel developed a structural model using steered molecular dynamic simulations describing how mechanical force switches the adhesion strength of FimH from low to high.
"Its quite remarkable, because this force-induced switching is happening at the tip of fimbriae along distance away from the cell membrane," Thomas says. "It makes you wonder how many more proteins exist that are switched mechanically – that is a fascinating area for research."
"We need to know how bacterial adhesion is altered by shear. FimH is the second adhesion protein, after fibronectin, for which we have established a structural mechanism for how nature uses mechanical force to regulate protein function. These adhesion proteins thus serve as nanoscale switches that convert mechanical stimuli into a chemical response," Vogel says
The forces described in the paper are within the range of shear force found within the body.
UWs Departments of Microbiology and Bioengineering do considerable research on bacterial adhesion and biomedical devices, respectively.
"A lot of bacteria have been studied under static conditions. What this paper should alert people to is that force profoundly affects the behavior of bacteria and their ability to bind to target cells," Vogel says.
For a video of bacterial movement during flow, see http://www.washington.edu/newsroom/news/images/lotohi.avi The video shows how E. coli moves around on while surrounded by low flow, but then locks down and grips its current position when heavy flow is present.
http://www.washington.edu/newsroom/news/images/fimhB.jpg The Cell paper shows how force stretches FimH. The active site is green, and the force stretches the segment that connects to the rest of the bacteria, in pink.
Walter Neary | EurekAlert!
Inflammation Triggers Unsustainable Immune Response to Chronic Viral Infection
24.10.2016 | Universität Basel
Resolving the mystery of preeclampsia
21.10.2016 | Universitätsklinikum Magdeburg
Ultrafast lasers have introduced new possibilities in engraving ultrafine structures, and scientists are now also investigating how to use them to etch microstructures into thin glass. There are possible applications in analytics (lab on a chip) and especially in electronics and the consumer sector, where great interest has been shown.
This new method was born of a surprising phenomenon: irradiating glass in a particular way with an ultrafast laser has the effect of making the glass up to a...
Terahertz excitation of selected crystal vibrations leads to an effective magnetic field that drives coherent spin motion
Controlling functional properties by light is one of the grand goals in modern condensed matter physics and materials science. A new study now demonstrates how...
Researchers from the Institute for Quantum Computing (IQC) at the University of Waterloo led the development of a new extensible wiring technique capable of controlling superconducting quantum bits, representing a significant step towards to the realization of a scalable quantum computer.
"The quantum socket is a wiring method that uses three-dimensional wires based on spring-loaded pins to address individual qubits," said Jeremy Béjanin, a PhD...
In a paper in Scientific Reports, a research team at Worcester Polytechnic Institute describes a novel light-activated phenomenon that could become the basis for applications as diverse as microscopic robotic grippers and more efficient solar cells.
A research team at Worcester Polytechnic Institute (WPI) has developed a revolutionary, light-activated semiconductor nanocomposite material that can be used...
By forcefully embedding two silicon atoms in a diamond matrix, Sandia researchers have demonstrated for the first time on a single chip all the components needed to create a quantum bridge to link quantum computers together.
"People have already built small quantum computers," says Sandia researcher Ryan Camacho. "Maybe the first useful one won't be a single giant quantum computer...
14.10.2016 | Event News
14.10.2016 | Event News
12.10.2016 | Event News
25.10.2016 | Earth Sciences
25.10.2016 | Power and Electrical Engineering
25.10.2016 | Process Engineering