Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Pulsed electrical fields destroy antibiotic-resistant bacteria infecting burn injuries

22.05.2014

MGH researchers test novel approach to disinfecting wounds in animal study

Application of a technology currently used to disinfect food products may help to get around one of the most challenging problems in medicine today, the proliferation of bacteria resistant to antibiotics and other antimicrobial drugs.

In a paper appearing in the June issue of the journal Technology and already released online, investigators from the Massachusetts General Hospital (MGH) Center for Engineering in Medicine describe how the use of microsecond-pulsed, high-voltage non-thermal electric fields successfully killed resistant bacteria infecting experimentally induced burns in mice, reducing bacterial levels up to 10,000-fold.

"Pulsed electrical field technology has the advantages of targeting numerous bacterial species and penetrating the full thickness of a wound," says Alexander Golberg, PhD, of the MGH Center for Engineering in Medicine (MGH-CEM), first author of the paper. "This could lead to a completely new means of burn wound disinfection without using antibiotics, which can increase bacterial resistance."

... more about:
»MGH »antibiotics »bacteria »infections »injuries »skin »species

About 500,000 individuals are treated for burn injuries in the U.S. each year. Standard burn treatment involves removal of burned tissue, skin grafts, and the application of antiseptic and antimicrobial dressings to prevent and treat infection. The growing prevalence of antibiotic-resistant bacteria – including strains of Acinetobacter baumannii and Staphylococcus aureus – is behind the frequent failure of antibiotic treatment, necessitating novel approaches to eliminate infecting pathogens.

Pulsed electrical fields (PEFs) have been used for decades to preserve food by destroying bacteria, presumably by causing the formation of large pores in the bacterial membrane; and more recently PEF's have been used to treat solid tumors. Members of the MGH research team, led by Martin Yarmush, MD, PhD, director of the MGH-CEM, have previously used PEF to study scarless skin regeneration and are currently investigating use of the technology to improve wound healing. Theorizing that the procedure could improve management of wound infection, the researchers designed the current study.

The investigators applied a multidrug resistant strain of A. baumannii to small third-degree burns that had been made on the backs of anesthetized mice. After 40 minutes, during which imaging of the fluorescent bacteria confirmed the established infection, the burned area was treated with an electrical field generated by placing the damaged skin between two electrodes. Each animal received two 40-pulse treatments five minutes apart, one group receiving 250 V/mm pulses and another receiving 500 V/mm pulses.

Images taken right after each treatment showed pronounced drops in bacterial levels. While images taken three hours later showed some bacterial regrowth, the overall results confirmed a persistent reduction in bacterial levels, ranging from a 500-fold reduction after 80 pulses at 250 V/mm volts to a more than 10,000-fold reduction after 80 pulses at 500 V/mm. The researchers also found that increasing the number of pulses per treatment had a greater effect on bacterial reduction than did increasing the strength of the electric field. Additional investigation is needed to confirm the safety of the tested voltage levels and the treatment's effectiveness against deep infections and other species of resistant bacteria.

"Currently available technologies have not been able to solve the problem of multidrug-resistant burn wound infections, and lasers are unable to treat infections deep within a wound because of the scattering and absorption of light," says Yarmush, who is senior author of the Technology paper. "Pulsed electric fields are a previously unexplored technique that has the potential to provide a chemical-free way of disinfecting burns and other wound infections."

###

Golberg is a research fellow in the MGH Department of Surgery, and Yarmush is a faculty member in MGH Surgery and in the Harvard-MIT Division of Health Science. Golberg previously presented this work at the 46th American Burn Association conference in March, where it received the 2014 Robert B. Lindberg Award for the best scientific paper by a nonphysician.

Additional co-authors of the Technology paper are Felix Broelsch, MD, Saiqa Khan, MD, and William Austen, Jr., MD, MGH Division of Plastic and Reconstructive Surgery; Daniela Vecchio, PhD, and Michael Hamblin, PhD, Wellman Center for Photomedicine at MGH; and Robert Sheridan, MD, Sumner Redstone Burn Center at MGH. The study was supported by the MGH Fund for Medical Discovery and Shriners Hospitals for Children grant 85120-BOS.

Massachusetts General Hospital, founded in 1811, is the original and largest teaching hospital of Harvard Medical School. The MGH conducts the largest hospital-based research program in the United States, with an annual research budget of more than $785 million and major research centers in AIDS, cardiovascular research, cancer, computational and integrative biology, cutaneous biology, human genetics, medical imaging, neurodegenerative disorders, regenerative medicine, reproductive biology, systems biology, transplantation biology and photomedicine.

Sue McGreevey | Eurek Alert!

Further reports about: MGH antibiotics bacteria infections injuries skin species

More articles from Health and Medicine:

nachricht A promising target for kidney fibrosis
21.04.2017 | Brigham and Women's Hospital

nachricht Stem cell transplants: activating signal paths may protect from graft-versus-host disease
20.04.2017 | Technische Universität München

All articles from Health and Medicine >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Making lightweight construction suitable for series production

More and more automobile companies are focusing on body parts made of carbon fiber reinforced plastics (CFRP). However, manufacturing and repair costs must be further reduced in order to make CFRP more economical in use. Together with the Volkswagen AG and five other partners in the project HolQueSt 3D, the Laser Zentrum Hannover e.V. (LZH) has developed laser processes for the automatic trimming, drilling and repair of three-dimensional components.

Automated manufacturing processes are the basis for ultimately establishing the series production of CFRP components. In the project HolQueSt 3D, the LZH has...

Im Focus: Wonder material? Novel nanotube structure strengthens thin films for flexible electronics

Reflecting the structure of composites found in nature and the ancient world, researchers at the University of Illinois at Urbana-Champaign have synthesized thin carbon nanotube (CNT) textiles that exhibit both high electrical conductivity and a level of toughness that is about fifty times higher than copper films, currently used in electronics.

"The structural robustness of thin metal films has significant importance for the reliable operation of smart skin and flexible electronics including...

Im Focus: Deep inside Galaxy M87

The nearby, giant radio galaxy M87 hosts a supermassive black hole (BH) and is well-known for its bright jet dominating the spectrum over ten orders of magnitude in frequency. Due to its proximity, jet prominence, and the large black hole mass, M87 is the best laboratory for investigating the formation, acceleration, and collimation of relativistic jets. A research team led by Silke Britzen from the Max Planck Institute for Radio Astronomy in Bonn, Germany, has found strong indication for turbulent processes connecting the accretion disk and the jet of that galaxy providing insights into the longstanding problem of the origin of astrophysical jets.

Supermassive black holes form some of the most enigmatic phenomena in astrophysics. Their enormous energy output is supposed to be generated by the...

Im Focus: A Quantum Low Pass for Photons

Physicists in Garching observe novel quantum effect that limits the number of emitted photons.

The probability to find a certain number of photons inside a laser pulse usually corresponds to a classical distribution of independent events, the so-called...

Im Focus: Microprocessors based on a layer of just three atoms

Microprocessors based on atomically thin materials hold the promise of the evolution of traditional processors as well as new applications in the field of flexible electronics. Now, a TU Wien research team led by Thomas Müller has made a breakthrough in this field as part of an ongoing research project.

Two-dimensional materials, or 2D materials for short, are extremely versatile, although – or often more precisely because – they are made up of just one or a...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Expert meeting “Health Business Connect” will connect international medical technology companies

20.04.2017 | Event News

Wenn der Computer das Gehirn austrickst

18.04.2017 | Event News

7th International Conference on Crystalline Silicon Photovoltaics in Freiburg on April 3-5, 2017

03.04.2017 | Event News

 
Latest News

NASA's Fermi catches gamma-ray flashes from tropical storms

25.04.2017 | Physics and Astronomy

Researchers invent process to make sustainable rubber, plastics

25.04.2017 | Materials Sciences

Transfecting cells gently – the LZH presents a GNOME prototype at the Labvolution 2017

25.04.2017 | Life Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>