"Our data are the first to show that transcutaneous immunization is an effective way to prevent experimental ear infections and lays the foundation for an effective, yet simple, inexpensive – and potentially transformative – way to deliver vaccines," says Laura Novotny, one of the study researchers.
Nontypeable Haemophilus influenzae (NTHi) is one of the three main bacterial causes of otitis media (OM), an infection or inflammation of the middle ear. OM is one of the most significant health problems for children in the United States, costing approximately $5 billion annually. It is estimated that 83% of all children will experience at least one ear infection prior to 3 years of age.
Currently infections are managed with antibiotics; however, the emergence of antibiotic-resistant microorganisms is of concern. Surgery to insert tubes through the tympanic membrane relieves painful symptoms, but the procedure is invasive and requires the child to be under general anesthesia. Thus, it is necessary to develop different ways to treat or preferably prevent this disease.
"We have designed several vaccine candidates which target proteins on the outer surface of this bacterium. Previous work in our lab showed that after immunization by injection, each of the three vaccine candidates prevented experimental ear infections caused by NTHi. In this study, we now wanted to test an alternative but potentially equally effective method to deliver a vaccine," says Novotny.
The method, known as transcutaneous immunization, involved placing a droplet of each vaccine onto the ear and rubbing it into the skin.
In this study, four groups of chinchillas were immunized with one of the three vaccine candidates. A fourth group received a placebo. Each vaccine was placed on the ears of chinchillas once a week for three weeks. All animals were then inoculated with NTHi through the nose and directly into the middle ears. Animals that received the vaccines were able to very rapidly reduce, or completely eliminate NTHi from the nose and ears, but animals that received a placebo did not.
This study was performed by Laura A. Novotny of Dr. Lauren O. Bakaletz's laboratory in the Center for Microbial Pathogenesis at The Research Institute at Nationwide Children's Hospital in Columbus, OH and in collaboration with Dr. John D. Clements, Department of Microbiology and Immunology, Tulane University, New Orleans, LA. Research was made possible by funding from the NIDCD/NIH R01 03915 & 007464. Data were presented at the 109th General Meeting of the American Society for Microbiology in Philadelphia, PA on May 21, 2009.
More information on this and other presentations can be found online in the 109th ASM General Meeting Press Kit at http://tinyurl.com/asmnewsroom or by contacting Jim Sliwa ( email@example.com or 202.942.9297) in the ASM Office of Communications. Follow media events at the meeting via Twitter at http://www.twitter.com/ASMNewsroom.
The American Society for Microbiology, headquartered in Washington, D.C., is the largest single life science association, with 42,000 members worldwide. Its members work in educational, research, industrial, and government settings on issues such as the environment, the prevention and treatment of infectious diseases, laboratory and diagnostic medicine, and food and water safety. The ASM's mission is to gain a better understanding of basic life processes and to promote the application of this knowledge for improved health and economic and environmental well-being.
Jim Sliwa | EurekAlert!
Penn vet research identifies new target for taming Ebola
12.01.2017 | University of Pennsylvania
The strange double life of Dab2
10.01.2017 | University of Miami Miller School of Medicine
Researchers from the University of Hamburg in Germany, in collaboration with colleagues from the University of Aarhus in Denmark, have synthesized a new superconducting material by growing a few layers of an antiferromagnetic transition-metal chalcogenide on a bismuth-based topological insulator, both being non-superconducting materials.
While superconductivity and magnetism are generally believed to be mutually exclusive, surprisingly, in this new material, superconducting correlations...
Laser-driving of semimetals allows creating novel quasiparticle states within condensed matter systems and switching between different states on ultrafast time scales
Studying properties of fundamental particles in condensed matter systems is a promising approach to quantum field theory. Quasiparticles offer the opportunity...
Among the general public, solar thermal energy is currently associated with dark blue, rectangular collectors on building roofs. Technologies are needed for aesthetically high quality architecture which offer the architect more room for manoeuvre when it comes to low- and plus-energy buildings. With the “ArKol” project, researchers at Fraunhofer ISE together with partners are currently developing two façade collectors for solar thermal energy generation, which permit a high degree of design flexibility: a strip collector for opaque façade sections and a solar thermal blind for transparent sections. The current state of the two developments will be presented at the BAU 2017 trade fair.
As part of the “ArKol – development of architecturally highly integrated façade collectors with heat pipes” project, Fraunhofer ISE together with its partners...
At TU Wien, an alternative for resource intensive formwork for the construction of concrete domes was developed. It is now used in a test dome for the Austrian Federal Railways Infrastructure (ÖBB Infrastruktur).
Concrete shells are efficient structures, but not very resource efficient. The formwork for the construction of concrete domes alone requires a high amount of...
Many pathogens use certain sugar compounds from their host to help conceal themselves against the immune system. Scientists at the University of Bonn have now, in cooperation with researchers at the University of York in the United Kingdom, analyzed the dynamics of a bacterial molecule that is involved in this process. They demonstrate that the protein grabs onto the sugar molecule with a Pac Man-like chewing motion and holds it until it can be used. Their results could help design therapeutics that could make the protein poorer at grabbing and holding and hence compromise the pathogen in the host. The study has now been published in “Biophysical Journal”.
The cells of the mouth, nose and intestinal mucosa produce large quantities of a chemical called sialic acid. Many bacteria possess a special transport system...
10.01.2017 | Event News
09.01.2017 | Event News
05.01.2017 | Event News
17.01.2017 | Earth Sciences
17.01.2017 | Materials Sciences
17.01.2017 | Architecture and Construction