Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

New research provides better understanding of endometriosis

05.06.2014

Macrophages present in shed endometrium in mice may suggest key role in lesion formation, according to new research published in The American Journal of Pathology

A mouse model of endometriosis has been developed that produces endometriosis lesions similar to those found in humans, according to a report published in The American Journal of Pathology. This model closely mirrors the human condition as an estrogen-dependent inflammatory disorder, and findings from the study suggest that macrophages present in shed endometrium contribute to the development of the lesions.

"One in 10 women of reproductive age have endometriosis; it is as common as asthma or diabetes, but it can take up to seven years to diagnose and there is an unmet clinical need for better treatments with fewer side effects," reported lead investigator Erin Greaves, PhD, MRC Centre for Reproductive Health, Queen's Medical Research Institute, The University of Edinburgh, when addressing the UK Parliament regarding her research.

The lack of a readily available, low-cost, and suitable animal model has hindered progress in the field. Nonhuman primates offer a physiologically relevant model, but their use is limited by cost and ethical concerns. Rat and mouse models have the advantage of lower cost and smaller size but have several disadvantages.

For example, mouse models often rely on suturing endometrial tissue onto the surface of pelvic organs since rodents do not naturally menstruate, raising the concern that tissue artificially placed in the pelvis may not simulate natural conditions or immune response.

The newly reported mouse model of endometriosis relies on the transplantation of menstrual endometrial tissue between genetically identical mice. In brief, a donor mouse is induced to undergo menstruation using estrogen and progesterone. The tissue that is shed from the uterus is removed and implanted into a recipient mouse, allowed to grow, and then removed and analyzed.

"We found that lesions recovered from a variety of sites in the peritoneum of the mice shared histologic similarities with human lesions, including the presence of hemosiderin, cytokeratin-positive epithelial cells, vimentin-positive stromal cells, and a well-developed vasculature. Most of the lesions had evidence of well-organized stromal and glandular structures," says Dr. Greaves. She noted other similarities including changes in the expression patterns of estrogen receptor α and β, also similar to what is found in patient biopsies.

By performing experiments using mice with green fluorescent protein-labeled macrophages in reciprocal transfers with wild-type mice, the researchers obtained evidence that the macrophages present in the shed endometrium survive and create a pro-inflammatory microenvironment that contributes to the formation of endometriotic lesions. "We are excited by these findings because the contribution of macrophages present in shed endometrium to the etiology of endometriotic lesions has not been studied in previous mouse models," comments Dr. Greaves.

The researchers hope that this model will inform future studies investigating the role of immune cells and menstrual tissue on the development of endometriosis, advance the understanding of mechanisms of the disease, and allow the identification and study of novel targets for therapy.

According to The World Endometriosis Society, endometriosis affects an estimated 176 million women worldwide. It is an inflammatory disorder where patches of endometrium-like tissue (the inner lining of the mammalian uterus) grow as lesions abnormally-located outside the uterine cavity. The tissue is thought to originate from endometrial fragments shed at menses. Characteristic inflammatory changes are seen such as increases in inflammatory mediators and tissue-resident immune cells. Women with endometriosis often complain of chronic, debilitating pelvic pain and infertility.

Eileen Leahy | Eurek Alert!
Further information:
http://www.elsevier.com

Further reports about: Elsevier Health disorder endometrial endometriosis immune inflammatory lesions macrophages menstrual stromal

More articles from Health and Medicine:

nachricht Antibiotic effectiveness imperiled as use in livestock expected to increase
27.03.2015 | Princeton University

nachricht A human respiratory tissue model to assess the toxicity of inhaled chemicals and pollutants
26.03.2015 | R&D at British American Tobacco

All articles from Health and Medicine >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Experiment Provides the Best Look Yet at 'Warm Dense Matter' at Cores of Giant Planets

In an experiment at the Department of Energy's SLAC National Accelerator Laboratory, scientists precisely measured the temperature and structure of aluminum as...

Im Focus: Energy-autonomous and wireless monitoring protects marine gearboxes

The IPH presents a solution at HANNOVER MESSE 2015 to make ship traffic more reliable while decreasing the maintenance costs at the same time. In cooperation with project partners, the research institute from Hannover, Germany, has developed a sensor system which continuously monitors the condition of the marine gearbox, thus preventing breakdowns. Special feature: the monitoring system works wirelessly and energy-autonomously. The required electrical power is generated where it is needed – directly at the sensor.

As well as cars need to be certified regularly (in Germany by the TÜV – Technical Inspection Association), ships need to be inspected – if the powertrain stops...

Im Focus: 3-D satellite, GPS earthquake maps isolate impacts in real time

Method produced by UI researcher could improve reaction time to deadly, expensive quakes

When an earthquake hits, the faster first responders can get to an impacted area, the more likely infrastructure--and lives--can be saved.

Im Focus: Atlantic Ocean overturning found to slow down already today

The Atlantic overturning is one of Earth’s most important heat transport systems, pumping warm water northwards and cold water southwards. Also known as the Gulf Stream system, it is responsible for the mild climate in northwestern Europe. 

Scientists now found evidence for a slowdown of the overturning – multiple lines of observation suggest that in recent decades, the current system has been...

Im Focus: Robot inspects concrete garage floors and bridge roadways for damage

Because they are regularly subjected to heavy vehicle traffic, emissions, moisture and salt, above- and underground parking garages, as well as bridges, frequently experience large areas of corrosion. Most inspection systems to date have only been capable of inspecting smaller surface areas.

From April 13 to April 17 at the Hannover Messe (hall 2, exhibit booth C16), engineers from the Fraunhofer Institute for Nondestructive Testing IZFP will be...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

World Conference On Regenerative Medicine 2015: Registration And Abstract Submission Now Open

25.03.2015 | Event News

University presidents from all over the world meet in Hamburg

19.03.2015 | Event News

10. CeBiTec Symposium zum Big Data-Problem

17.03.2015 | Event News

 
Latest News

Two Most Destructive Termite Species Forming Superswarms in South Florida

27.03.2015 | Agricultural and Forestry Science

ORNL-Led Team Demonstrates Desalination with Nanoporous Graphene Membrane

27.03.2015 | Materials Sciences

Coorong Fish Hedge Their Bets for Survival

27.03.2015 | Ecology, The Environment and Conservation

VideoLinks
B2B-VideoLinks
More VideoLinks >>>