The innovation represents a way to enhance commercially available diagnostic devices that use paper-strip assays like those that test for diabetes and pregnancy.
"With current systems that use paper test strips you can measure things like pH or blood sugar, but you can't perform more complex chemical assays," said Babak Ziaie, a Purdue University professor of electrical and computer engineering and biomedical engineering. "This new approach offers the potential to extend the inexpensive paper-based systems so that they are able to do more complicated multiple analyses on the same piece of paper. It's a generic platform that can be used for a variety of applications.”
Findings are detailed in a research paper published online this week in the journal Lab on a Chip.
Current lab-on-a-chip technology is relatively expensive because chips must be specifically designed to perform certain types of chemical analyses, with channels created in glass or plastic and tiny pumps and valves directing the flow of fluids for testing.
The chips are being used for various applications in medicine and research, measuring specific types of cells and molecules in a patient's blood, monitoring microorganisms in the environment and in foods, and separating biological molecules for laboratory analyses. But the chips, which are roughly palm-size or smaller, are difficult to design and manufacture.
The new technique is simpler because the testing platform will be contained on a disposable paper strip containing patterns created by a laser. The researchers start with paper having a hydrophobic - or water-repellant - coating, such as parchment paper or wax paper used for cooking.
"Since the hydrophobic agent is already present throughout the thickness of the paper, our method creates islands of hydrophilic patterns," Ziaie said. "This modified surface has a highly porous structure, which helps to trap and localize chemical and biological aqueous reagents for analysis. Furthermore, we've selectively deposited silica microparticles on patterned areas to allow diffusion from one end of a channel to the other."
Those microparticles help to wick liquid to a location where it would combine with another chemical, called a reactant, causing it to change colors and indicating a positive or negative test result.
Having a patterned hydrophilic surface is needed for many detection methods in biochemistry, such as enzyme-linked immunosorbent assay, or ELISA, used in immunology to detect the presence of an antibody or an antigen in a sample, Ziaie said.
To demonstrate the new concept, the researchers created paper strips containing arrays of dots dipped in luminol, a chemical that turns fluorescent blue when exposed to blood.
"Then we sprayed blood on the strips, showing the presence of hemoglobin," said Ziaie, whose research is based at the Birck Nanotechnology Center in the university's Discovery Park. "This is just a proof of concept."
Laser modification is known to alter the "wettability" of materials by causing structural and chemical changes to surfaces. However, this treatment has never before been done on paper, he said.
The researchers performed high-resolution imaging and spectroscopic analysis to study the mechanism behind the hydrophobic-hydrophilic conversion of laser-treated parchment paper.
The new approach is within a research area called paper microfluidics.
"Other techniques in paper microfluidics are more complicated," Ziaie said.
For example, other researchers have developed a method that lays down lines of wax or other hydrophobic material on top of untreated, hydrophilic paper.
"Our process is much easier because we just use a laser to create patterns on paper you can purchase commercially and it is already impregnated with hydrophobic material," Ziaie said. "It's a one-step process that could be used to manufacture an inexpensive diagnostic tool for the developing world where people can't afford more expensive analytical technologies."
The strips might be treated with chemicals that cause color changes when exposed to a liquid sample, with different portions of the pattern revealing specific details about the content of the sample. One strip could be used to conduct dozens of tests, he said.
The strips might be inserted into an electronic reader, similar to technology used in conventional glucose testers. Color changes would indicate the presence or absence of specific chemical compounds.
The research paper was written by graduate students Girish Chitnis, Zhenwen Ding and Chun-Li Chang; Cagri A. Savran, an associate professor of mechanical engineering, biomedical engineering and electrical and computer engineering; and Ziaie.
The National Science Foundation funded the work.
The researchers have patented the technique and it is available for licensing through Joseph Trebley, senior project manager for the Purdue Research Foundation Office of Technology Commercialization, at 765-588-3832, firstname.lastname@example.org (http://www.prf.org/otc).
Writer: Emil Venere, 765-494-4709, email@example.com
Source: Babak Ziaie, 765-494-0725, firstname.lastname@example.org
Note to Journalists: An electronic copy of the research paper is available from Emil Venere, 765-494-4709, email@example.com
Emil Venere | EurekAlert!
Researchers release the brakes on the immune system
18.10.2017 | Rheinische Friedrich-Wilhelms-Universität Bonn
Norovirus evades immune system by hiding out in rare gut cells
12.10.2017 | University of Pennsylvania School of Medicine
University of Maryland researchers contribute to historic detection of gravitational waves and light created by event
On August 17, 2017, at 12:41:04 UTC, scientists made the first direct observation of a merger between two neutron stars--the dense, collapsed cores that remain...
Seven new papers describe the first-ever detection of light from a gravitational wave source. The event, caused by two neutron stars colliding and merging together, was dubbed GW170817 because it sent ripples through space-time that reached Earth on 2017 August 17. Around the world, hundreds of excited astronomers mobilized quickly and were able to observe the event using numerous telescopes, providing a wealth of new data.
Previous detections of gravitational waves have all involved the merger of two black holes, a feat that won the 2017 Nobel Prize in Physics earlier this month....
Material defects in end products can quickly result in failures in many areas of industry, and have a massive impact on the safe use of their products. This is why, in the field of quality assurance, intelligent, nondestructive sensor systems play a key role. They allow testing components and parts in a rapid and cost-efficient manner without destroying the actual product or changing its surface. Experts from the Fraunhofer IZFP in Saarbrücken will be presenting two exhibits at the Blechexpo in Stuttgart from 7–10 November 2017 that allow fast, reliable, and automated characterization of materials and detection of defects (Hall 5, Booth 5306).
When quality testing uses time-consuming destructive test methods, it can result in enormous costs due to damaging or destroying the products. And given that...
Using a new cooling technique MPQ scientists succeed at observing collisions in a dense beam of cold and slow dipolar molecules.
How do chemical reactions proceed at extremely low temperatures? The answer requires the investigation of molecular samples that are cold, dense, and slow at...
Scientists from the Max Planck Institute of Quantum Optics, using high precision laser spectroscopy of atomic hydrogen, confirm the surprisingly small value of the proton radius determined from muonic hydrogen.
It was one of the breakthroughs of the year 2010: Laser spectroscopy of muonic hydrogen resulted in a value for the proton charge radius that was significantly...
17.10.2017 | Event News
10.10.2017 | Event News
10.10.2017 | Event News
20.10.2017 | Information Technology
20.10.2017 | Materials Sciences
20.10.2017 | Interdisciplinary Research